Biotin carboxylase

Tảo lục nước ngọt (Haematococcus pluvialis Flotow ) được chứng minh là nguyên liệu khởi đầu cho việc sản xuất nhiên liệu sinh học, chứa nhiều lipid cùng với astaxanthin, một chất màu có giá trị kinh tế cao. Trong nghiên cứu này, sự tích lũy lipid ở vi tảo H. pluvialis được nuôi cấy trong bình chứa môi trường lỏng Bold's Basal (BB) và sục khí được khảo sát trong thời gian 12 tuần.

6p vitomato2711 11-03-2020 41 5   Download

Pyruvate carboxylase (PC) fromBacillus thermodenitrificans was engineered in such away that the polypeptide chainwas divided into two, between the biotin carboxylase (BC) and carboxyl transferase (CT) domains. The two proteins thus formed, PC-(BC) and PC-(CT+BCCP), retained their catalytic activity as assayed by biotin-dependent ATPase and oxamate-dependent oxalacetate decarboxylation, for the former and the latter, respectively. Neither activity was dependent on acetyl-CoA, in sharp contrast to the complete reaction of intact PC. ...

10p dell39 03-04-2013 40 5   Download

Nitric oxide (NO) is a signaling molecule that affects a myriad of processes in plants. However, the mechanistic details are limited. NO post-transla-tionally modifies proteins by S-nitrosylation of cysteines. The soluble S-nitrosoproteome of a medicinal, crassulacean acid metabolism (CAM) plant,Kalanchoe pinnata, was purified using the biotin switch technique.

11p media19 06-03-2013 47 4   Download

Biotin Biotin is a water-soluble vitamin that plays a role in gene expression, gluconeogenesis, and fatty acid synthesis and serves as a CO 2 carrier on the surface of both cytosolic and mitochondrial carboxylase enzymes. The vitamin also functions in the catabolism of specific amino acids (e.g., leucine). Excellent food sources of biotin include organ meat such as liver or kidney, soy, beans, yeast, and egg yolks; however, egg white contains the protein avidin, which strongly binds the vitamin and reduces its bioavailability.

5p konheokonmummim 03-12-2010 75 5   Download