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Blastodermal cells
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The spotted-wing Drosophila (Drosophila suzukii) is a widespread invasive pest that causes severe economic damage to fruit crops. The early development of D. suzukii is similar to that of other Drosophilids, but the roles of individual genes must be confirmed experimentally. Cellularization genes coordinate the onset of cell division as soon as the invagination of membranes starts around the nuclei in the syncytial blastoderm.
10p
vihagrid
30-01-2023
6
3
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The FC method using specific nuclear dye DRAQ5 was used to separate BCs from cellular debris. Only 1.82 ± 0.27 % cells were DRAQ5 positive. On the other hand, increase of total events (21 ± 3.30 %) of AnV positive cells without DRAQ5 dye was recorded. Moreover, the affinity of yolk to AnV by FC was also observed (10.45 ± 2.59 %). Significant differences among the groups (P < 0.001) were recorded.
5p
mudbound
10-12-2021
13
1
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Producing transgenic chickens with chicken blastodermal cells (cBCs) is inefficient due to the extremely low germline transmission capacity of cBCs.
11p
vihamax2711
21-04-2020
10
0
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