![](images/graphics/blank.gif)
Blood mononuclear cells
-
Ebook "DNA damage detection in situ, ex vivo, and in vivo: Methods and protocols" presents all major assays used in molecular and cell biology for the labeling of DNA damage in situ, ex vivo, and in vivo. It brings together recently introduced techniques, as well as those established earlier, which detect and quantify DNA damage at the scales ranging from subcellular to the level of a whole, living organism.
328p
dongmelo
27-05-2024
7
2
Download
-
Systemic and local profiles have each been associated with asthma, but parsing causal relationships between system-wide and airway-specific processes can be challenging. We sought to investigate systemic and airway processes in asthma and their causal relationships.
12p
vicwell
29-02-2024
2
2
Download
-
This study aims to study PASD1 protein as a potential target for CRC immunotherapy. A total of 90 CRC and polyps tissue samples were investigated for PASD1 RNA and protein expression using a real-time polymerase chain reaction and immunohistochemical staining, respectively. Matched patients’ peripheral blood mononuclear cells were pulsed with PASD1 peptides and measured for immunogenicity, cell cytotoxicity, and cytokine assays.
17p
lyhuyenthu
31-01-2023
6
2
Download
-
The warming climate is causing livestock to experience heat stress at an increasing frequency. Holstein cows are particularly susceptible to heat stress because of their high metabolic rate. Heat stress negatively affects immune function, particularly with respect to the cell-mediated immune response, which leads to increased susceptibility to disease.
11p
vidarwin
23-02-2022
14
1
Download
-
A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps.
15p
vialfrednobel
29-01-2022
6
0
Download
-
Robust protocols and automation now enable large-scale single-cell RNA and ATAC sequencing experiments and their application on biobank and clinical cohorts. However, technical biases introduced during sample acquisition can hinder solid, reproducible results, and a systematic benchmarking is required before entering large-scale data production.
16p
viarchimedes
26-01-2022
10
0
Download
-
In this study, we presented the results of immune cells expansion from peripheral blood of nine patients belonging to three types of leading cancer in Vietnam: lung, liver and gastric cancers. These cells were expanded by using BINKIT developed by Dr. Terunuma (Biotherapy Institute in Japan), which was proved to efficiently induce the number of selective cells and activate them to kill cancer cells.
11p
guitaracoustic02
08-12-2021
12
1
Download
-
The present study, therefore, explored whether migration and apoptosis of peripheral blood mononuclear cells (PBMCs) and ALL blasts in high glucose conditions is regulated by A20. To this end, ALL blasts from blood samples of fifteen patients and PBMCs from healthy individuals in the absence of A20 were examined. Gene expression profile was determined by quantitative RT-PCR, cell apoptosis by flow cytometry, and cell migration by a transwell migration assay. As a result, the expression of A20 was inactivated in ALL blasts.
8p
spiritedaway36
25-11-2021
11
0
Download
-
The accumulation of mitochondrial DNA (mtDNA) mutations, and the reduction of mtDNA copy number, both disrupt mitochondrial energetics, and may contribute to aging and age-associated phenotypes. However, there are few genetic and epidemiological studies on the spectra of blood mtDNA heteroplasmies, and the distribution of mtDNA copy numbers in different age groups and their impact on age-related phenotypes.
14p
vilarryellison
29-10-2021
1
0
Download
-
Milk yield for Holstein cows has doubled over five decades due to genetic selection and changes to management, but the molecular mechanisms that facilitated this increase are mostly unknown. Epigenetic modifications to the cattle genome are a plausible molecular mechanism to cause variation in milk yield and our objective was to describe genome-wide DNA methylation patterns in peripheral blood mononuclear cells (PBMC) from mature Holstein dairy cows with variable milk yield.
12p
vitzuyu2711
29-09-2021
7
1
Download
-
Bovine viral diarrhea virus (BVDV) is an economically important viral pathogen of domestic and wild ruminants. Apart from cattle, small ruminants (goats and sheep) are also the susceptible hosts for BVDV. BVDV infection could interfere both of the innate and adaptive immunity of the host, while the genes and mechanisms responsible for these effects have not yet been fully understood.
14p
visilicon2711
20-08-2021
6
1
Download
-
Anti-CD3 immunotherapy was initially approved for clinical use for renal transplantation rejection prevention. Subsequently, new generations of anti-CD3 antibodies have entered clinical trials for a broader spectrum of therapeutic applications, including cancer and autoimmune diseases.
14p
visilicon2711
20-08-2021
11
1
Download
-
Tối ưu hóa quá trình thu nhận tế bào đơn nhân máu ngoại vi (peripheral blood mononuclear cell - PBMC) và quá trình chuyển nạp tạo tế bào CAR-T. Đối tượng và phương pháp: Máu ngoại vi từ người hiến tặng khỏe mạnh được tiến hành tách PBMC bằng FicollPaque.
8p
viansan2711
27-07-2021
22
1
Download
-
Many plant parts have been widely used in the treatment of immune diseases in Vietnam, yet just few of them are known about their mechanism of action. The goal of this study was to investigate the in vitro inhibitory effects of in vitro inhibitory effect of crude ethanol extracts and fractions of thirteen medicinal plants on proliferation of human peripheral blood mononuclear cells (PBMCs), interleukin-2 secretion and antioxidant activity. PBMCs were extracted from the whole blood of healthy volunteers.
7p
nguaconbaynhay11
07-04-2021
10
1
Download
-
The course of disease for patients with idiopathic pulmonary fibrosis (IPF) is highly heterogeneous. Prognostic models rely on demographic and clinical characteristics and are not reproducible. Integrating data from genomic analyses may identify novel prognostic models and provide mechanistic insights into IPF.
12p
vimontana2711
05-04-2021
11
3
Download
-
This work was done to demonstrate the effect of peripheral blood mononuclear cells on skeletal muscle regeneration after induced ischemia /reperfusion injury.
12p
chauchaungayxua10
19-03-2021
12
1
Download
-
Dipeptidyl peptidase-IV (DPP-IV) is suggested to contribute to the pathogenesis of several autoimmune diseases. The aim of this study was to evaluate the association of DPP-IV presence in blood plasma and mononuclear cells with the disease activity in rheumatoid arthritis (RA).
7p
vioregon2711
22-02-2021
10
1
Download
-
Recent research has suggested that cardiotrophin-like cytokine factor 1 (CLCF1) may be an important regulator of bone homeostasis. Furthermore, a whole gene chip analysis suggested that the expression levels of CLCF1 in the peripheral blood mononuclear cells (PBMCs) were downregulated in postmenopausal women with osteoporosis.
9p
vivermont2711
28-01-2021
10
2
Download
-
A fundamental characteristic of multicellular organisms is the specialization of functional cell types through the process of differentiation. These specialized cell types not only characterize the normal functioning of different organs and tissues, they can also be used as cellular biomarkers of a variety of different disease states and therapeutic/vaccine responses.
10p
viconnecticut2711
29-10-2020
11
1
Download
-
Acute lymphoblastic leukemia (ALL) is a heterogeneous group of malignant disorders derived from B- or T-cell lymphoid progenitor cells. ALL often is refractory to or relapses after treatment; thus, novel targeted therapy for ALL is urgently needed. In the present study, we initially found that the level of SIRT1, a class III histone deacetylase, was higher in primary ALL cells from patients than in peripheral blood mononuclear cells from healthy individuals.
15p
vialabama2711
21-09-2020
14
1
Download
CHỦ ĐỀ BẠN MUỐN TÌM
![](images/graphics/blank.gif)