Bovine milk

The present work reports isolation and characterization of a highly glycosylated protein from bovine milk fat globule membranes, known as PAS III. Partial amino-acid sequencing of the purified protein allowed construction of degenerate oligonucleotide primers, enabling isolation of a full-length cDNA encoding a protein of 330 amino-acid residues. N-terminal amino-acid sequencing of derived peptides and the purified protein confirmed 76% of the sequence and demonstrated presence of a cleavable signal peptide of 23 residues, leaving a mature protein of 307 amino acids.

9p system191 01-06-2013 50 4   Download

Previous work from this laboratory has shown that the spectral and functional properties of a prokaryotic xanthine dehydrogenase fromComamonas acidovoransshow some similarities to those of the well-characterized eukaryotic enzymes isolated from bovine milk and from chicken liver [Xiang, Q. & Edmondson, D.E. (1996) Biochemistry35, 5441–5450]. Therefore, this system was chosen to study the factors involved in the expression of functional recombinant enzyme in Escherichia colito provide insights into the assembly of the functional Mo-pyranopterin center....

11p fptmusic 12-04-2013 40 2   Download

Lactoferrin (LF) is an iron-binding glycoprotein of the innate host defence system. To elucidate the role ofN-linked glycosylation in protection of LF against proteolysis, we compared the tryptic susceptibility of human LF (hLF) variants from human milk, expressed in human 293(S) cells or in the milk of transgenic mice and cows. The analysis revealed that recombinant hLF (rhLF) with mutations Ile130fiThr and Gly404fiCys was about twofold more susceptible than glycosylated and unglycosylated variants with the naturally occurring Ile130 and Gly404....

7p dell39 03-04-2013 43 5   Download

b-lactoglobulin (LG) is a major bovine milk protein, containing a central calyx and a second exosite beyond the calyx to bind vitamin D; however, the biological function of LG in transporting vitamin D remains elusive. Crystallographic findings from our previous study showed the exosite to be located at the pocket between the a-helix and b-strand I.

15p viettel02 22-02-2013 26 2   Download

Milk provides nutritional, immunological and developmental components for newborns. Whereas identification of such components has been per-formed by targeting proteins and free oligosaccharides, structural and func-tional analyses of the N-glycome of milk glycoproteins are scarce.

13p cosis54 09-12-2012 46 2   Download

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học quốc tế đề tài: Mapping of a milk production quantitative trait locus to a 1.056 Mb region on bovine chromosome 5 in the Fleckvieh dual purpose cattle breed

11p toshiba18 08-11-2011 66 5   Download

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú y đề tài: Bovine renal lipofuscinosis: Prevalence, genetics and impact on milk production and weight at slaughter in Danish cattle...

9p coxanh_1 22-10-2011 45 5   Download

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú y đề tài: A study of bovine mastitis, milking procedures and management practices on 25 Estonian dairy herds...

6p coxanh_1 22-10-2011 62 5   Download