Bp’s involvement

Xem 1-19 trên 19 kết quả Bp’s involvement
  • Soybean homeobox gene GmSBH1 has previously been proven to be involved in response to high temperature and humidity (HTH) stress. To investigate its expression patterns and active cis-elements, a 2040-bp 5’-upstream genomic DNA fragment of GmSBH1, named GmSBH1P, was isolated by PCR walking and characterized. Sequence analysis revealed that the fragment contains a series of cis-acting elements related to stress responses.

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  • The mangrove plant Kandelia candel is a type of woody halophyte that grows in tropical and subtropical ocean intertidal zones and exhibits a high salt tolerance. In this study, 61,970 unigenes were obtained from the roots of 60-day-old K. candel seedlings treated with 0 (control), 200, 400, and 600 mM NaCl for 3 days with an N50 of 1510 bp. Moreover, 454, 311, and 2663 genes were differentially expressed under 200, 400, and 600 mM NaCl treatments, respectively.

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  • 1-Aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO), is one of the core enzymesin the ethylene biosynthesis pathway, which catalyzes the final step in ethylene biosynthesis converting ACC to ethylene, cyanide, CO2, dehydroascorbate and water with inputs of Fe(II), ascorbic, bicarbonate and oxygen.In the present study, a 1288 bp cDNA sequence fragment of the ACO gene (designated as GbACO) was isolated from ginkgo (Ginkgo biloba) leaves by using reverse transcriptase-polymerase chain reaction (RT-PCR) with two PCR primers designed according to the ginkgo transcriptome data.

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  • Retinoid signalling is crucial to vertebrate embryonic development. The Cyp26B1 is one of the key catabolic enzymes, involved in the inactivation of all-trans retinoic acid and helps in the maintaining of retinoic acid availability for retinoid signalling. In the present study, for the first time, we cloned and characterized the full coding sequence of Cyp26B1 gene in Clarias magur. The full-length cDNA sequence of Cyp26B1 gene was 1782 bp (ORF 1533 bp) encoding 511 amino acids. Signal peptide analysis revealed that Cyp26B1 is a secretory protein as reported in other vertebrates.

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  • Chronic myeloid leukemia (CML) is a malignancy that evolves through a multi-step process. Alternative splicing of several genes has been linked to the progression of the disease, but involvement of alternations in splicing profiles has not been reported. RNA-seq of peripheral blood mononuclear cell (PBMC) samples characterized the differentially expressed and spliced transcripts in five CML chronic phase (CP) and five blast phase (BP) patients, and five healthy controls.

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  • The study was undertaken with an objective to evaluate a rapid PCR based method for detection of adulteration of buffalo milk in cow milk at minimum level of detection. This method utilizes primers targeting the mitochondrial encoded 12S rRNA gene as the target for species identification. PCR assay involve use of three different primers. Reverse primers specific for cow and buffalo complementary to the gene fragment of 12S rRNA along with the common forward primer. The cow specific primer, along with the common forward primer, yields a cow specific amplicon of 346 bp in the 12S rRNA gene.

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  • 4E-BP1 is a family member of eIF4E binding proteins (4E-BPs) which act as the suppressors of cap-dependent translation of RNA via competitively associating with cap-bound eIF4E. RNA translation regulation is an important manner to control the cellular responses to a series of stress conditions such as ionizing radiation (IR)–induced DNA damage response and cell cycle controlling.

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  • Adenosine is implicated in the modulation of cardiovascular responses either at the peripheral or at central level in experimental animals. However, there are no dedicated reviews on the involvement of adenosine in mediating the hypotensive response of centrally administered clonidine in general and specifically in aortically barodenervated rats (ABD). The conscious ABD rat model exhibits surgically induced baroreflex dysfunction and exaggerated hypotensive response, compared with conscious sham-operated (SO) rats.

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  • Salinity is one of the major abiotic stresses, and it limits rice production in many worldwide areas. A lot of attention has been paid to developing rice salinity tolerance to cope with climate change. This study is based on the genome sequence databases of 36 Vietnamese landraces. The candidate gene, SRWD2, has been predicted and identified. The salt responsive WD40 protein 2 is involved in salt tolerance yield of nine rice landraces.

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  • The aim of the present study was to elucidate the role of osteoblasts in bisphosphonates-related osteonecrosis of the jaw (BRONJ). The specific objective was to evaluate the effect on osteoblasts of two nitrogen-containing BPs (zoledronate and alendronate) and one non-nitrogen-containing BP (clodronate) by analyzing modulations in their expression of genes essential for osteoblast physiology.

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  • DRE (dehydration responsive element)/CRT (C-repeat) is a cis-acting element that involves in gene expression responsive to abiotic stress in higher plants. To date, all well known DREBP transcription factors in Arabidopsis, rice, maize and other plants regulate gene expression in response to drought, high-salt and cold stresses by binding specifically to DRE/CRT.

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  •  The intestinal fatty acid-binding protein (I-FABP) shows binding specificity for long-chain fatty acids and is proposed to be involved in uptake of dietary fatty acids and their intracellular transport. We have determined the sequence of the gene encoding I-FABP in zebrafish. The zebrafish I-FABP gene contains four exons interrupted by three introns. Radiation hybrid mapping assigned the I-FABP gene to linkage group 1. A 924 bp sequence 5Vupstream of the initiation codon in the I-FABP gene contained several putative cis-acting regulatory elements.

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  • Transglutaminase (TGase) catalyses the post-translational modification of proteins by transamidation of available glutamine residues. While several TGase genes of fish and arthropods have been cloned and appear to have similar structures to those of mammals, no homologous gene has been found in lower eukaryotes. We have cloned the acellular slime mold Physarum polycephalum TGase cDNA using RT-PCR with degenerated primers, based on the partial amino acid sequence of the purified enzyme. The cDNA contained a 2565-bp ORF encoding a 855-residue polypeptide.

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  • Thebrain fattyacid-bindingprotein (B-FABP) is involved in brain development and adult neurogenesis. We have deter-mined the sequence of the gene encoding the B-FABP in zebrafish. The zebrafish B-FABP gene spans 2370 bp and contains four exons interruptedby three introns. The coding sequence of zebrafishB-FABP gene is identical to its cDNA sequence and the coding capacity of each exon is the same as that for the human and mouse B-FABP genes. A 1249 bp sequence 5¢ upstream of exon 1 of the zebrafish B-FABP gene was cloned and sequenced. ...

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  • The rhesus (Rh) blood group antigens are of considerable importance in transfusion medicine as well as in newborn or autoimmune hemolytic diseases due to their high antigenicity. We identified a major DNaseI hypersensitive site at the 5¢ flanking regions of bothRHDandRHCEexon 1. A 34 bp fragment located at)191 to)158 from a translation start posi-tion, and containing the TCCCCTCCC sequence, was involved in enhan-cing promoter activity, which was assessed by luciferase reporter gene assay.

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  • This monograph (a preferable term here to ‘book’, I believe) was conceived after I had done a good deal of broadcasting, within the UK and internationally, on the Gulf Coast oil spill. Time is always limited in a broadcast, and facts and valid perspectives need to be got across succinctly to the exclusion of shallow comments which hardly leave a viewer or listener any better informed. I like to go to a broadcast having made a few jottings from news sources which as well as being possible material for the broadcast have attuned my mind to the topic shortly before...

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  • The risk management processes described in this book had their genesis well over 20 years ago when I accepted a position at the University of Southampton. There I met and worked with Dr Chris Chapman, already an acknowledged expert in project risk, with an established relationship with BP and an extensive client base in Canada. Chris involved me in his consulting activities in North America, primarily associated with quantitative risk analyses of large projects in the hydroelectric and the oil and gas industries....

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  • The number of DNA sequences and transcription factors that regulate transcription is much greater than originally anticipated. Most genes contain at least 15–20 discrete regulatory elements within 300 bp of the transcription start site. This densely packed promoter region often contains binding sites for ubiquitous transcription factors such as CAAT box/enhancer binding protein (C/EBP), cyclic AMP response element–binding (CREB) protein, selective promoter factor 1 (Sp-1), or activator protein 1 (AP-1).

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  • Linear IgA Disease Linear IgA disease, once considered a variant form of dermatitis herpetiformis, is actually a separate and distinct entity. Clinically, these patients may resemble individuals with DH, BP, or other subepidermal blistering diseases. Lesions typically consist of papulovesicles, bullae, and/or urticarial plaques predominantly on central or flexural sites. Oral mucosal involvement occurs in some patients. Severe pruritus resembles that seen in patients with DH.

    pdf6p konheokonmummim 03-12-2010 64 2   Download



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