Breast cancer genetics

The research project deals with two different types of data for two separate analysis. The first analysis deals with normalised RNA-seq breast cancer data where machine learning techniques are used to classify and identify the biomarker of cancer. Second analysis deals with raw DNA methylated leukemia samples to determine the mutations.

102p runthenight04 02-02-2023 7 4   Download

Matrix metalloproteinase (MMP)-3 inhibited human MDA-MB-231 breast cancer cell invasion through recon-stituted basement membranein vitro. Inhibition of invasion was dependent upon plasminogen and MMP-3 activation, was impaired by the peptide MMP-3 inhibitor Ac-Arg-Cys-Gly-Val-Pro-Asp-NH2 and was associated with: rapid MMP-3-mediated plasminogen degradation to microplas-minogen and angiostatin-like fragments;

8p research12 23-04-2013 35 3   Download

A sialylated lacto-N-neotetraose (Sial-lNnT) structural unit was identified and structurally characterized in the lipo-polysaccharide (LPS) from the genome-sequenced strain Road (RM118) of the human pathogenHaemophilus influ-enzaegrown in the presence of sialic acid. A combination of molecular genetics, MS and NMR spectroscopy techniques showed that this structural unit extended from the proximal heptose residue of the inner core regionof theLPSmolecule.

11p research12 23-04-2013 36 2   Download

Humanb2 -glycoprotein I (b2 GPI), also known as apolipo-protein H, has been implicated in haemostasis and the pro-duction ofanti-phospholipid antibodies. There is a wide range ofinterindividual variation in b2GPI plasma levels that is thought to be under genetic control, but itsmolecular basis remains unknown. To understand the genetic basis of b2GPI variation, we analyzed the 5¢ flanking region ofthe b2GPIgene for mutation detection by DHPLC and identi-fied a point mutation at the transcriptional initiation site ()1CfiA) with a carrier frequency of 12.1%. ...

9p tumor12 20-04-2013 36 3   Download

The currently applied reaction structure in stoichiometric flux balance mod-els for the nonoxidative branch of the pentose phosphate pathway is not in accordance with the established ping-pong kinetic mechanism of the enzymes transketolase (EC 2.2.1.1) and transaldolase (EC 2.2.1.2). Based upon the ping-pong mechanism, the traditional reactions of the nonoxida-tive branch of the pentose phosphate pathway are replaced by metabolite specific, reversible, glycolaldehyde moiety (C2 ) and dihydroxyacetone moi-ety (C3) fragments producing and consuming half-reactions. ...

13p fptmusic 12-04-2013 42 3   Download

Genetic and proteomic approaches have led to the identification of 32 pro-teins, collectively called peroxins, which are required for the biogenesis of peroxisomes. Some are responsible for the division and inheritance of per-oxisomes; however, most peroxins have been implicated in the topogenesis of peroxisomal proteins. Peroxisomal membrane and matrix proteins are synthesized on free ribosomes in the cytosol and are imported post-trans-lationally into pre-existing organelles (Lazarow PB & Fujiki Y (1985) Annu Rev Cell Biol1, 489–530 [1])....

11p awards 06-04-2013 48 4   Download

Analysis of dopamine transporter gene expression pattern ) generation of DAT-iCre transgenic mice ´ Marc Turiault1,*, Sebastien Parnaudeau1,*, Aude Milet1, Rosanna Parlato2, Jean-Denis Rouzeau1, Monique Lazar1 and Francois Tronche1 ¸ ` 1 CNRS UMR7148, Molecular Genetics, Neurophysiology and Behavior, College de France, Institut de Biologie, Paris, France 2 Molecular Biology of the Cell I, German Cancer Research Center, Heidelberg, Germany Keywords Cre ⁄ loxP system; dopaminergic cells; dopamine transporter; gene expression; transgenic mice Correspondence F.

10p galaxyss3 19-03-2013 44 3   Download

Candida albicansis an opportunistic human pathogen with remark-able phenotypic plasticity. Its diploid cells can undergo an epi-genetic phase-transition termed the white-opaque switching. Whereas white cells have a round shape and are unable to mate, opaque cells have an oval shape and are mating-competent.

88p galaxyss3 19-03-2013 28 5   Download

In eukaryotes, meiosis leads to genetically variable gametes through recom-bination between homologous chromosomes of maternal and paternal ori-gin. Chromatin organization following meiotic recombination is critical to ensure the correct segregation of homologous chromosomes into gametes. However, the mechanism of chromatin organization after meiotic recombi-nation is unknown.

10p media19 06-03-2013 43 2   Download

Similar to blood types, human plasma haptoglobin (Hp) is classified into three phenotypes: Hp 1-1, 2-1 and 2-2. They are genetically inherited from two alleles Hp 1and Hp 2(represented in bold), but only the Hp 1-1 phenotype is found in almost all animal species.

13p media19 06-03-2013 31 3   Download

A major contributing factor to the development of breast cancer is decreased functional expression of breast cancer susceptibility gene 1, BRCA1. Another key contributor to tumorigenesis is hypoxia. Here we show that hypoxia increased the nuclear localization of BRCA1 in MCF-7 and MDA-MB-468 human breast cancer cell lines without changing its steady-state expression level.

10p media19 04-03-2013 49 2   Download

Breast cancer cells exhibit excessive proteolysis, which is responsible for extensive extracellular matrix degradation, invasion and metastasis. Besides other proteases, lysosomal cysteine protease cathepsin B has been impli-cated in these processes and the impairment of its intracellular activity was suggested to reduce harmful proteolysis and hence diminish progression of breast tumors.

12p media19 04-03-2013 50 2   Download

The primary cause of cystic fibrosis (CF), the most frequent fatal genetic disease in Caucasians, is deletion of phenylalanine at position 508 (F508del), located in the first nucleotide-binding domain (NBD1) of the CF transmembrane conductance regulator (CFTR) protein.

13p vinaphone15 25-02-2013 32 2   Download

Mutations in leucine-rich repeat kinase 2 (LRRK2) (PARK8) are associ-ated with both familial and sporadic forms of Parkinson’s disease. Most studies have shown that LRRK2 mutations may explain between 5% and 13% of familial and 1–5% of sporadic Parkinson’s disease. Importantly, a common recurrent mutation (G2019S) located in the kinase domain has been reported across most ethnic populations, with the highest prevalence among Ashkenazi Jews and North African Arabs.

9p viettel02 20-02-2013 42 5   Download

he tumor suppressor, breast cancer susceptibility gene 1 (BRCA1), plays an integral role in the maintenance of genome stability and, in particular, the cellular response to DNA damage. Here, the emerging role of BRCA1 in nonhomologous end-joining-mediated DNA repair following DNA dam-age will be reviewed, as well as the activation of apoptotic pathways.

7p viettel02 19-02-2013 49 3   Download

Apoptosis or programmed cell death is an inherent part of the development and homeostasis of multicellular organisms. Dysregulation of apoptosis is implicated in the pathogenesis of diseases such as cancer, neurodegenera-tive diseases and autoimmune disorders. Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) is able to induce apoptosis by binding death receptor (DR)4 (TRAIL-R1) and DR5 (TRAIL-R2), which makes TRAIL an interesting and promising therapeutic target.

13p mobifone23 18-01-2013 41 3   Download

This study characterized the human apolipoprotein H [APOH; b2-glycopro-tein I (b2GPI)] promoter and its variants byin vitro functional experiments and investigated their relationship with human plasmab2GPI levels. We examined the individual effects of 12 APOHpromoter single nucleotide polymorphisms in the 5¢ flanking region ofAPOH(1.4 kb) on luciferase activity in COS-1 cells and HepG2 cells and their impact on plasma...

13p mobifone23 18-01-2013 48 3   Download

Tham khảo luận văn - đề án 'báo cáo khoa học: solution parameters modulating dna binding specificity of the restriction endonuclease ecorv', luận văn - báo cáo, báo cáo khoa học phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả

15p cosis54 05-01-2013 56 4   Download

Organisms subject to changing environmental conditions or experimental protocols show complex patterns of responses. The design principles behind these patterns are still poorly understood. Here, modular metabolic control analysis is developed to deal with large changes in branched pathways.

14p cosis54 05-01-2013 49 3   Download

Proteins might aggregate into ordered or amorphous structures, utilizing relatively short sequence stretches, usually organized in b-sheet-like assem-blies. Here, we attempt to list all available software, developed during the last decade or so, for the prediction of such aggregation-prone stretches from protein primary structure, without distinguishing whether these algo

8p cosis54 05-01-2013 44 3   Download