Broth microdilution assay

In the conventional broth microdilution assay, the optical density (OD) of drug dilutions, inoculated with microorganisms after sufficient incubation is taken into consideration, which correlates with the most metabolically active cells in the mid log phase of growth. The particles present in the liposomes and other particulate solutions interfere with the OD measurement and produce an erratic result. Resazurin method is more reliable and realistic as it depends on metabolic activity of cells rather than OD of particulate matter present in the samples.

8p nguathienthan8 20-10-2020 14 2   Download

Staphylococcus aureus and Pseudomonas aeruginosa are part of the human microbiota and are also important bacterial pathogens, for which therapeutic options are lacking nowadays. The combined administration of corticosteroids and antimicrobials is commonly used in the treatment of infectious diseases to control inflammatory processes and to minimize potential toxicity of antimicrobials, avoiding sequelae.

7p kequaidan1 16-11-2019 22 0   Download