Capitulum explants cultured

An efficient protocol was developed for commercial multiplication of gerbera using young capitulum as explant across different varieties of gerbera. Media for culture establishment, shoot regeneration, multiplication were standardized. Mercuric chloride treatment (0.1%) for 2 minutes was found to be the best surface sterilant for capitulum explants resulting in the minimum per cent contaminated growing cultures (17.27%).

14p gaocaolon5 14-06-2020 11 2   Download

An effective protocol for in vitro proliferation and multiplication of Gerbera cv. Partrizia was established during 2015-17. Among the two stages initially used for culture initiation, capitulum explants cultured at immature stage responded better than the mature stage. Best establishment of immature capitulum explants and more number of quality shoots was obtained on modified MS medium supplemented with 10 mg/l BAP and 1 mg/l IAA. Half strength MS medium containing NAA (0.5 mg/l) + IBA (0.5 mg/l) was best for root induction.

6p nguathienthan4 18-04-2020 14 1   Download