cDNA sequencing
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The study of human B cell response to dengue virus (DENV) infection is critical to understand serotype-specific protection and the cross-reactive sub-neutralizing response. Whereas the first is beneficial and thus represents the ultimate goal of vaccination, the latter has been implicated in the development of severe disease, which occurs in a small, albeit significant, fraction of secondary DENV infections.
19p vioraclene 31-03-2024 2 2 Download
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Ebook "Transgenic microalgae as green cell factories (Advances in experimental medicine and biology, Volume 616)" book includes chapters focussing on biotechnological applications of transgenic microalgae. The enormous amount information generated by the genomic or cDNA sequencing projects and its implications on the development of new biotechnological applications of microalgae are analysed in Chapter 6.
145p duongthandue0501 28-02-2024 8 1 Download
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Part 1 book "PCR Protocols" includes content: Long range PCR with a DNA polymerase fusion, isolation of genomic insertion sites of proviruses using splinkerette PCR based procedures, lariat dependent nested PCR for flanking sequence determination, a global single-Cell cDNA amplification method for quantitative microarray analysis,...and other contents.
171p oursky06 17-10-2023 5 1 Download
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Misidentification of the chicken leptin gene has hampered research of leptin signaling in this species for almost two decades. Recently, the genuine leptin gene with a GC-rich (~70%) repetitive-sequence content was identified in the chicken genome but without indicating its genomic position.
8p vinarcissa 21-03-2023 3 1 Download
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(BQ) Ebook Plant Reverse Genetics: Methods and Protocols – Part 1 presents the following content: Chapter 1 : analysis of high-throughput sequencing data; chapter 2: identification of plant micrornas using expressed sequence tag analysis; chapter 3: microarray data analysis; chapter 4: setting up reverse transcription quantitative-pcr experiments; chapter 5: virus-induced gene silencing in nicotiana benthamiana and other plant species; chapter 6: agroinoculation and agroinfiltration: simple tools for complex gene function analyses; chapter 7: full-length cdna overexpressor gene hunting syst...
134p runordie7 30-08-2022 7 2 Download
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Bài viết Nghiên cứu xác định các vùng EST-SSR đặc trưng của loài sâm Ngọc Linh (Panax vietnamensis Ha & Grushv) bằng phương pháp giải trình tự gen thế hệ mới trình bày việc giải mã trình tự hệ gen phiên mã (cDNA) của sâm Ngọc Linh bằng công nghệ giải trình tự thế hệ mới (Next-generation sequencing) trên hệ thống Illumina HiSeqTM 4000 nhằm xác định các vùng SSR (Simple sequence repeat) đặc trưng của loài sâm này.
5p viaudi 04-08-2022 20 4 Download
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Single-cell RNA-seq technologies require library preparation prior to sequencing. Here, we present the first report to compare the cheaper BGISEQ-500 platform to the Illumina HiSeq platform for scRNA-seq. We generate a resource of 468 single cells and 1297 matched single cDNA samples, performing SMARTer and Smart-seq2 protocols on two cell lines with RNA spike-ins.
8p vigalileogalilei 27-02-2022 11 1 Download
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Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3′ cDNA libraries for dozens of samples, requiring just 2 hours of hands-on time.
15p vigalileogalilei 27-02-2022 8 2 Download
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Interferon regulatory factor 2 (IRF2) is an important transcription factor, which can regulate the IFN response and plays a role in antiviral innate immunity in teleost. In the present study, the full-length cDNA sequence of IRF2 (CcIRF2) was characterized in common carp (Cyprinus carpio L.).
12p vidarwin 23-02-2022 9 1 Download
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Quantifying the benefit offered by transcript assembly with Scallop-LR on single-molecule long reads
Single-molecule long-read sequencing has been used to improve mRNA isoform identification. However, not all single-molecule long reads represent full transcripts due to incomplete cDNA synthesis and sequencing length limits.
18p vielonmusk 30-01-2022 6 0 Download
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Gene annotations, such as those in GENCODE, are derived primarily from alignments of spliced cDNA sequences and protein sequences. The impact of RNA-seq data on annotation has been confined to major projects like ENCODE and Illumina Body Map 2.0.
14p vialfrednobel 29-01-2022 10 0 Download
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Nonsense-mediated mRNA decay (NMD) is a eukaryotic, translationdependent degradation pathway that targets mRNAs with premature termination codons and also regulates the expression of some mRNAs that encode full-length proteins.
23p viarchimedes 26-01-2022 16 0 Download
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Pseudogenes are gene copies presumed to mainly be functionless relics of evolution due to acquired deleterious mutations or transcriptional silencing. Using deep fulllength PacBio cDNA sequencing of normal human tissues and cancer cell lines, we identify here hundreds of novel transcribed pseudogenes expressed in tissue-specific patterns.
15p viarchimedes 26-01-2022 7 0 Download
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Long-read sequencing of full-length cDNAs enables the detection of structures of aberrant splicing isoforms in cancer cells. These isoforms are occasionally translated, presented by HLA molecules, and recognized as neoantigens.
30p viarchimedes 26-01-2022 13 0 Download
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Crustacean hyperglycaemic hormone (CHH) is a neuropeptide that was originally identified in the X-organ/sinus gland complex of the eyestalks (ESs) in crustaceans. Several CHH isoforms and spliced variants were later identified in other tissues, and their functions have still not been completely unveiled. In this study, the identification and characterisation of the conventional CHH prepropeptide from the ESs of the littoral crab, Carcinus aestuarii, via rapid amplification of cDNA ends was reported.
10p dolomite36 30-12-2021 7 0 Download
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Flowers of safflower (Carthamus tinctorius L.) have been widely used in traditional Chinese medicine because of their ability to improve cerebral blood flow. The major active ingredients in safflower are flavonoids. To date, few flavonoid biosynthesis genes have been cloned from this species. In our previous research, four isoform chalcone isomerase genes were screened. In this study, a new chalcone isomerase gene (designated as CtCHI-N) was cloned from a safflower cDNA library. A 696-bp cDNA for CtCHI-N was cloned, and its sequence was subsequently analyzed.
8p tudichquannguyet 29-11-2021 7 0 Download
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The cDNA encoding lipase enzyme was cloned from Olea europaea var. europaea cv. Gemlik leaves using the RACE method. The full nucleotide sequence was 1546 bp long and encoded a putative protein of 412 amino acid long protein. The amino acid sequence showed identity to Triacylglycerol Lipase 1 sequences from different plants. A phylogenetic tree was constructed showing relations with various plant lipases.
8p tudichquannguyet 29-11-2021 11 1 Download
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Rheum palmatum has been widely developed in traditional Chinese medicine. One of the major active compounds in R. palmatum is flavonoids. Until now, few genes involved in flavonoid biosynthesis have been isolated from this medicinal plant. In this study, a new chalcone isomerase gene (RpCHI) was cloned from an RNA-seq library of R. palmatum. The 714bp RpCHI cDNA was cloned and sequenced.
8p tudichquannguyet 29-11-2021 12 1 Download
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Genotyping of the human leucocyte antigen (HLA) is indispensable for various medical treatments. However, unambiguous genotyping is technically challenging due to high polymorphism of the corresponding genomic region. Next-generation sequencing is changing the landscape of genotyping. In addition to high throughput of data, its additional advantage is that DNA templates are derived from single molecules, which is a strong merit for the phasing problem.
12p vilarryellison 29-10-2021 11 0 Download
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Current library preparation protocols for Illumina HiSeq and MiSeq DNA sequencers require ≥2 nM initial library for subsequent loading of denatured cDNA onto flow cells. Such amounts are not always attainable from samples having a relatively low DNA or RNA input; or those for which a limited number of PCR amplification cycles is preferred (less PCR bias and/or more even coverage).
10p vibeauty 23-10-2021 11 1 Download