Cell culturing

Circulating tumor cell (CTC) clusters play a critical role in carcinoma metastasis. However, the rarity of CTC clusters and the limitations of capture techniques have retarded the research progress. In vitro CTC clusters model can help to further understand the biological properties of CTC clusters and their clinical significance.

17p vishanshan 27-06-2024 1 1   Download

Understanding the metabolic changes in colorectal cancer (CRC) and exploring potential diagnostic biomarkers is crucial for elucidating its pathogenesis and reducing mortality. Cancer cells are typically derived from cancer tissues and can be easily obtained and cultured.

13p vishanshan 27-06-2024 1 1   Download

The study was carried out to clone sheep cytokine genes for protein expression in mammalian cell culture. Once expressed, it is hypothesized that these proteins, when added to a primary mast cell culture, will allow the growth of these cells.

15p viambani 18-06-2024 2 0   Download

In this research, we fabricated α-mangostin-loaded liposomes from soybean lecithin and cholesterol to enhance the solubility in water of α-mangostin and reduce side effects in the treatment of cancer.

11p viamancio 04-06-2024 2 0   Download

Considerable effort and time is allocated to introducing cell culture and fermentation technology to undergraduate students in academia, generally through a range of courses in industrial biotechnology and related disciplines. Similarly, a large number of textbooks are available to describe the appli-tions of these technologies in industry.

240p vimeyers 29-05-2024 2 1   Download

Ebook "Handbook of industrial cell culture: Mammalian, microbial, and plant cells" attempts to link these common approaches, while also delineating those specific aspects of cell types, to give the reader not only an overview of the best current practices, but also of today’s evolving technologies, with examples of both their practical applications and their future potential.

548p dongmelo 27-05-2024 5 3   Download

Ebook "Plant cell culture protocols (Third edition)" is taught around the world as part of undergraduate and graduate courses in agronomical and biological sciences. However, the apparent simplicity of cell culture technology should not lead to mistakingly consider it as trivial. It would be very diffi cult to understand modern plant biotechnology without in vitro cell cultures and even today, the application of recombinant DNA technology to important crops is hampered by the poor embryogenic or morphogenetic response of in vitro cultured tissues.

445p dongmelo 27-05-2024 5 2   Download

Part 1 book "Microbiology - A laboratory manual" includes contents: Effectiveness of hand washing experiment; culture transfer techniques experiment; techniques for isolation of pure cultures experiment; cultural characteristics of microorganisms; microscopic examination of stained cell preparations experiment; microscopic examination of living microorganisms using a hanging drop preparation or a wet mount.

281p dianmotminh01 17-05-2024 5 3   Download

Ebook "Hormonal carcinogenesis V" information gathered from cell-free systems, cell cultures, animal models, and human studies, together will (1) provide important insights to our understanding of hormonal cancer causation, development, and prevention; (2) be the primary objective of these Symposia.

647p ladongphongthanh1008 22-04-2024 2 1   Download

Ebook "Tight junctions" highlight the importance of understanding TJ physiology in order to develop effective strategies for the treatment of pathological conditions such as cancer and autoimmune diseases. The first chapter of this book describes the evolution of the transporting epithelium from primitive metazoans to mammalian epithelia. Then, Chapters 2, 3, 4, 5, 6 and 7 review the characteristics and functionality of difi^erent proteins of theTJ studied in cell culture models, natural epithelia, mutant mice and heritable human diseases.

235p ladongphongthanh1008 22-04-2024 4 2   Download

Ebook "Bacterial physiology and metabolism" focuses on research on bacteria, as well as metabolism of carbohydrates, fermentation, and oxidation of acids. The book first offers information on nutrition and growth of bacterial cultures, including requirements for growth, nutritional classification of bacteria, measurement of bacterial growth, and synchronous growth of bacterial cultures.

553p tracanhphuonghoa1007 22-04-2024 1 1   Download

Eucalyptus is one of the important plant species for paper pulp, fuel wood, and timber. In this study, a hybrid Eucalyptus, U16 line, was investigated for rapid in vitro propagation. The apical and adventitious shoots served as initial explants. These were free-disease symptoms that were collected for surface sterilization.

8p vijaychest 24-04-2024 2 2   Download

This study aimed to establish culture medium changing system to improve the efficiency of bovine somatic cell nuclear transfer protocol. Our results showed that using the culture medium of Takahashi and First 1992 produced the cloned bovine blastocyst at the highest rate (9.4%, P < 0.05) among different modified synthetic oviduct fluid (mSOF) media.

4p visergey 02-04-2024 4 0   Download

Preclinical studies require models that recapitulate the cellular diversity of human tumors and provide insight into the drug sensitivities of specific cellular populations. The ideal platform would enable rapid screening of cell type-specific drug sensitivities directly in patient tumor tissue and reveal strategies to overcome intratumoral heterogeneity.

15p vibransone 28-03-2024 3 2   Download

Human pancreatic islets are a central focus of research in metabolic studies. Transcriptomics is frequently used to interrogate alterations in cultured human islet cells using single-cell RNA-sequencing (scRNAseq). We introduce single-nucleus RNA-sequencing (snRNA-seq) as an alternative approach for investigating transplanted human islets.

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Ebook "Mesenchymal stem cells: Basics and clinical application I" provides readers with contents including: prospective isolation and characterization of human bone marrow-derived MSCs; urine as a source of stem cells; expansion of mesenchymal stem/stromal cells under xenogenic-free culture conditions; adipose-derived mesenchymal stem cells - biology and potential applications;...

198p coduathanh1122 27-03-2024 2 1   Download

Genetic suppression occurs when the deleterious effects of a primary “query” mutation, such as a disease-causing mutation, are rescued by a suppressor mutation elsewhere in the genome. Methods To capture existing knowledge on suppression relationships between human genes, we examined 2,400 published papers for potential interactions identified through either genetic modification of cultured human cells or through association studies in patients.

19p vicwell 29-02-2024 4 1   Download

Ebook "Q’eqchi’ maya reproductive ethnomedicine" have an extensive pharmacopoeia of medicinal plants used traditionally for reproductive health and fertility, utilizing more than 60 plant species for these health treatments. Ten species were selected for investigation of their estrogenic activity using a reporter gene assay. Nine of the species were estrogenic, four of the species were also antiestrogenic, and two of the extracts were cytotoxic to the MCF-7 breast cancer cell line.

134p manmanthanhla0201 26-02-2024 2 1   Download

Ebook "Atlas of human pluripotent stem cells in culture" is a comprehensive collection of illustrated techniques complemented by informative and educational captions examining what good quality cells look like and how they behave in various environments. Examples of perfect cultures are compared side-by-side to less-than-perfect and unacceptable examples of human embryonic and induced pluripotent stem cell colonies.

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Ebook "Genomics and systems biology of mammalian cell culture (Advances in biochemical engineering/biotechnology, Volume 127)" have gathered nine chapters written by authors from both academia and industry which present recent development and different facets of genomic and systems-biological approaches applied to mammalian cell cultures. The book will give some impulse and inspiration to the genomics and systems biology of mammalian cell cultures.

305p manmanthanhla0201 26-02-2024 1 1   Download