Cell formation

In this study, the fruiting bodies of the X. nigripes were collected to isolate, propagate in the liquid medium, and then cultured to induce the fruiting body formation in the rice medium. The results showed that the X. nigripes was successfully isolated on PDAY (Potato Dextrose Agar Yeast Extract) medium, and the mycelium and spore of the X. nigripes had unique characteristics of the species.

7p vibenya 31-12-2024 1 1   Download

Luận văn nghiên cứu về vấn đề tối ưu áp dụng trong bố trí sản xuất. Để tiếp bước đến nền công nghiệp 4.0, sắp xếp lịch trong sản xuất/công tác nhằm giảm quá trình “đợi” nhau trong các công đoạn và tận dụng được nhiều tài nguyên phục vụ sản xuất. Bài toán trong luận văn nghiên cứu cụ thể là bài toán về CF (cell formation). Theo đó, việc hình thành các ô (cell) là hoán vị các công việc và các “máy” thực hiện.

105p capheviahe28 01-03-2021 39 6   Download

The objective of the study was to find the explant resources, the type and concentration of plant growth regulators (PGRs), as well as the in vitro culture conditions suitable for different morphogenesis processes - callus induction, direct embryogenesis, shoot and root formation, etc. The growth and development of in vitro Ngoc Linh ginseng derived from thin cell layer (TCL) was examined in Quang Nam to assess the adaptability of in vitro Ngoc Linh plantlets in its natural territory, compared to those growing in Bidoup - Nui Ba National Park, Lam Dong.

27p capheviahe27 23-02-2021 21 5   Download

The objective of the study was to find the explant resources, the type and concentration of plant growth regulators (PGRs), as well as the in vitro culture conditions suitable for different morphogenesis processes - callus induction, direct embryogenesis, shoot and root formation, etc. The growth and development of in vitro Ngoc Linh ginseng derived from thin cell layer (TCL) was examined in Quang Nam to assess the adaptability of in vitro Ngoc Linh plantlets in its natural territory, compared to those growing in Bidoup - Nui Ba National Park, Lam Dong.

27p xacxuoc4321 09-07-2019 35 5   Download

Câu 1. Để thực hiện định dạng đường viền cho bảng dữ liệu nằm trong tập tin danh sách lớp, chọn bảng dữ liệu đó, nhấn chuột vào thực đơn lệnh Format - Cells. Trong hộp thoại Format Cells, chọn thẻ A. Font B. Pattern C. Border D. Alignment Câu 2. Địa chỉ $AC$3 là địa chỉ A. Tuyệt đối B. Tương đối C. Biểu diễn sai D. Hỗn hợp Câu 3. Để xem nhanh trang bảng tính trước khi in, bạn sử dụng thao tác nào dưới đây A. Vào menu View, chọn Zoom B. Vào menu File, chọn Web Page Preview C. Vào menu File, chọn...

4p vanhungnls 15-05-2013 394 48   Download

We report here the direct evidence for the formation of alternative DNA structures in a plasmid DNA, termed pTIR10, containing a 0.23-kb pyrimidine/purine-biased (Pyr/Pur) stretch isolated from the rat genome. Long Pyr/Pur sequences are abundant in eukaryotic genomes, and they may modulate the biological activity of genes and genomes via formation of various types of triplex-related structures. The plasmid DNA in sodium acetate buffer (pH 4.35) was deposited on APS-modified mica, and after drying it was imaged with an atomic force microscope in air. ...

5p system191 01-06-2013 34 3   Download

The insect ecdysteroid receptor consists of a heterodimer between EcR and the RXR-orthologue, USP. We addressed the question of whether this heterodimer, like all other RXR heterodimers, may be formed in the absence of ligand and whether ligand promotes dimerization. We found that C-terminal protein fragments that comprised the ligand binding, but not the DNA binding domain of EcR and USP and which were equipped with the activation or DNA binding region of GAL4, respectively, exhibit a weak ability to interact spontaneously with each other.

9p system191 01-06-2013 30 3   Download

Cys341 of carboxypeptidase Y, which constitutes one side of the solvent-accessible surface of the S1 binding pocket, was replaced with Gly, Ser, Asp, Val, Phe or His by site-directed mutagenesis. Kinetic analysis, using Cbz-dipeptide substrates, revealed that polar amino acids at the 341 position increased Km whereas hydrophobic amino acids in this position tended to decrease Km.

6p system191 01-06-2013 40 4   Download

The serpins are of general protein chemical interest due to their ability to undergo a large conformational change consisting of the insertion of the reactive centre loop (RCL) as strand 4 of the central b sheet A. To make space for the incoming RCL, the ‘shutter region’ opens by the b strands 3A and 5A sliding apart over the underlying a helix B. Loop insertion occurs during the formation of complexes of serpins with their target serine proteinases and during latency transition. This type of loop insertion is unique to plasminogen activator inhibitor-1 (PAI-1).

10p system191 01-06-2013 42 4   Download

The centromere–kinetochore complex is a highly specialized chromatin domain that both mediates and monitors chromosome–spindle interactions responsible for accurate partitioning of sister chromatids to daughter cells. Centromeres are distinguished from adjacent chromatin by specific patterns of histone modification and the presence of a centromere-specific histone H3 variant (e.g. CENP-A). Centromere-proximal regions usually correspond to sites of avid and persistent sister chromatid cohesion mediated by the conserved cohesin complex.

15p research12 01-06-2013 50 4   Download

Department of Medical Chemistry, Kochi Medical School, Nankoku, Japan; 2Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan; 3Department of Anatomy and Cell Biology, Kochi Medical School, Nankoku, Japan We previously generated mice lacking aromatase activity by targeted disruption of Cyp19 (ArKO mice), and reported phenotypes of the female mice, showing hemorrhage formation and follicular depletion in the ovary, diminution in uterine size, and bone loss...

9p research12 01-06-2013 42 4   Download

The ÔMediterraneanÕ variant of glucose-6-phosphate dehydrogenase (G6PD) deficiency is due to the C563CT point mutation, leading to replacement of Ser with Phe at position 188, resulting in acute haemolysis triggered by oxidants. Previous work has shown increased formation of altered aspartate residues in membrane proteins during cell ageing and in response to oxidative stress in normal erythrocytes. These abnormal residues are specifically recognized by the repair enzyme L-isoaspartate (D-aspartate) protein O-methyltransferase (PCMT; EC 2.1.1.77). ...

8p research12 01-06-2013 39 4   Download

The naturally synchronous plasmodia of myxomycetes synthesize poly(b-L-malic acid), which carries out cell-specific functions. In Physarum polycephalum, poly(b-L-malate) [the salt form of poly(b-L-malic acid)] is highly concentrated in the nuclei, repressing DNA synthetic activity of DNA polymerases by the formation of reversible complexes. To test whether this inhibitory activity is cell-cycle-dependent, purified DNA polymerase a of P.

6p research12 01-06-2013 62 3   Download

ZntA, a bacterial zinc-transporting P-type ATPase, is homologous to two human ATPases mutated in Menkes and Wilson diseases. To explore the roles of the bacterial ATPase residues homologous to those involved in the humandiseases, we have introduced several pointmutations into ZntA. The mutants P401L, D628A and P634L corres-pond to the Wilson disease mutations P992L, D1267A and P1273L, respectively.

8p research12 29-04-2013 44 3   Download

Here we show, for the ®rst time, thein vitro formation of ®lamentous aggregates of phosphorylated tau protein in SH-SY5Y human neuroblastoma cells. The formation of suchaberrant aggregates, similar to thoseoccurringinvivoin Alzheimer's disease and other tauopathies, requires okadaic acid, a phosphatase inhibitor, to increase the level of phos-phorylated tau, and hydroxynonenal, a product of oxidative stress that selectively adducts and modi®es phosphorylated tau.

6p research12 29-04-2013 32 2   Download

Tripeptidyl-peptidase II (TPP II) is a large (Mr106 ) tripeptide-releasing enzyme with an active site of the subtil-isin-type. Compared with other subtilases, TPP II has a 200 amino-acid insertion between the catalytic Asp44 and His264 residues, and is active as anoligomeric complex. This study demonstrates that the insert is important for the formation of the active high-molecular mass complex.

6p research12 29-04-2013 37 3   Download

Hyaluronan-binding protein 1 (HABP1), a ubiquitous multifunctional protein, interacts with hyaluronan, globular head of complement component 1q (gC1q), and clustered mannose and has been shown to be involved in cell sig-nalling.In vitro, this recombinant protein isolated from human ®broblast exists in di€erent oligomeric forms, as is evident from the results of various independent techniques in near-physiological conditions.

9p research12 29-04-2013 29 4   Download

In hydra, differentiated ectodermal cells of the foot region containaperoxidaseactivity that canbeusedas amarker for foot-specific differentiation processes. Because the expres-sion of the gene coding for the peroxidase must be tightly regulated during foot-specific differentiation, characteriza-tion of the protein and cloning of the corresponding gene shouldprovide valuable tools for gettingdeeper insights into the regulation of foot-specific differentiation.

10p research12 23-04-2013 42 2   Download

The interactionofmany lytic cationic antimicrobial peptides with their target cells involves electrostatic interactions, hydrophobic effects, and the formation of amphipathic sec-ondary structures, such asahelices orbsheets. We have shown in previous studies that incorporating
30% D-aminoacids intoashortahelical lyticpeptidecomposedof leucine and lysine preserved the antimicrobial activity of the parent peptide, while the hemolytic activity was abolished.

12p research12 23-04-2013 35 2   Download

Mammalian tubulins and actins attain their native con-formation following interactions with CCT (the cytosolic chaperonin containing t-complex polypeptide 1). To study the b-tubulin folding in lower eukaryotes, an isotype of b-tubulin (b-T1) from the Antarctic ciliate Euplotes focardii, was expressed in Escherichia coli. Folding analysis was performed by incubation of the 35 S-labeled, denatured b-T1 in the presence, or absence, of purified rabbit CCT and cofactor A, a polypeptide that stabilizes folded monomericb-tubulin. ...

7p tumor12 22-04-2013 51 3   Download