Chalcalburnus tarichi

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Impacts of some metal ions on glutathione s-transferase in the liver of Chalcalburnus tarichi: an endemic species of Lake Van

In this study, we determined the in vitro inhibitory effects of metal ions such as Cu2+, Cd2+, Ag+, and Co2+ on GST enzyme activity. For this aim, GST was purified from C. tarichi Pallas liver with 37.36% yield and 29.304 EU/mg specific activity using the chromatographic method. The Vmax values of liver GST were determined for CDNB and GSH to be 1.245 and 0.562 EU/mL, respectively, and the Km values were found 0.89 and 0.06 mM, respectively, using the Lineweaver–Burk plot.

8p lyhuyenthu 31-01-2023 3 2 Download

Properties and some in vitro studies of 6-phosphogluconate dehydrogenase purified from the liver of Chalcalburnus tarichi, the only fish living in Lake Van’s highly alkaline water (pH 9.8)

In this study, some enzymology parameters and some antibiotics affecting the enzyme activity of 6- phosphogluconate dehydrogenase (6PGD) extracted from t the livers of fish from Lake Van (Chalcalburnus tarichi) were investigated because it is an important enzyme-producing NADPH, a reductive power, protecting the cell against the oxidative agents by producing reduced glutathione. The crude enzyme solution was obtained by using the affinity chromatography method.

9p langthannam 29-12-2021 11 0 Download