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Characteristics of phytase enzyme

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  • Two hundred ten (210) of one day old commercial broiler chicks (VenCobb 500) were divided into five (T0, T1, T2, T3&T4) groups of 42 birds in each with three replicate of 14 broilers in each with the aim to see the effect of microbial phytase enzyme on the tibia characteristics and biochemistry in blood serum of broilers. For said purpose, the birds of (T1, T2, T3&T4) groups were feed with basal diets having (250FTU, 500FTU, 750FTU & 1000FTU/kg) respectively, while T0 group was feed without any phytase.

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  • Cereals, legumes and oilseed crops are grown over 90% of the world’s harvested area. Together they serve as a major source of nutrients for the animal kingdom. An important constituent of seeds of these crops is phytic acid (myo-inositol 1, 2, 3, 4, 5, 6-hexakisphosphate; Ins P6). The salt form, phytate, is an anhydrous storage form of phosphate accounting for more than 80% of the total phosphorus in cereals and legumes. In contrast to other organo-phosphate molecules, phytate contains a high phosphate content, which results in a high negative charge over a wide pH range.

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  • In this study, an extracellular alkali-thermostable phytase producing bacteria, Bacillus subtilis B.S.46, were isolated and molecularly identified using 16S rRNA sequencing. Response surface methodology was applied to study the interaction effects of assay conditions to obtain optimum value for maximizing phytase activity. The optimization resulted in 137% (4.627 U/mL) increase in phytase activity under optimum condition (56.5 C, pH 7.30 and 2.05 mM sodium phytate). The enzyme also showed 60–73% of maximum activity at wide ranges of temperature (47–68 C), pH (6.3–8.

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