Chitinase production
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Acidic mammalian chitinase (AMCase) has been implicated in various pathophysiological conditions including asthma, allergic inflammation and food processing. AMCase is most active at pH 2.0, and its activity gradually decreases to up to pH 8.
9p vihermione 06-01-2023 17 2 Download
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These isolates were screened, in vitro, according to their capacities to produce plant growth promoting substance i.e. IAA, phosphate, potassium solubilization, chitinase activities and hydrogen cyanide. Results indicated that 36 bacterial isolates showed IAA production, 25 HCN production, 57 chitinase activities, 39 phosphate and 105 potassium solubilizers.
8p schindler 30-12-2021 9 0 Download
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A total of 19 bacterial endophytes were isolated from leaves of cotton (Gossypium hirsutum) and characterized for plant growth promoting traits viz., siderophores production, phosphate solubilization, IAA and ammonia production; and preliminarily screened for chitinase, gelatinase, protease and lipase.
13p gaocaolon9 22-12-2020 27 3 Download
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In the present study the isolated endophytic actinomycete from the banana root and leaf tissues have been tested for their bioactivities and quantitative production of extracellular chitinase against phytopathogenic fungi Fusarium oxysporum that cause economically important disease in banana crop and to identify potent antagonistic strain.
10p trinhthamhodang1215 23-09-2020 8 1 Download
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Plant growth promoting bacteria has become of great interest to promote the crop growth and protect from phytopathogens. Total of 100 bacterial isolates were obtained form 45 different samples of rhizosphere soil as well as sugarcane parts. After extensive screening, eight isolates found as potential for multiple PGP traits were further investigated for cellulase, chitinase, HCN production potential and antifungal activity. Isolates F271 and F373were found to produce chitinase.
13p kethamoi6 29-06-2020 11 1 Download
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Chitinases are involved in chitin catabolism of fungi and bacteria but are also widely distributed in plant Kingdome as defense molecules. Chitinases are known for their diversity and vast potential uses viz., biocontrol agents for plant pathogens and insects, biopesticides, chitioligosaccharides, single cell protein production from chitinous wastes etc. Plant chitinases have been classified in various classes (Class I-VII) based on their domain organizations.
13p nguaconbaynhay4 22-03-2020 22 2 Download
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The filamentous fungus Paecilomyces is currently being developed as a biocontrol agent against plant parasitic nematodes, which often cause black leaf disease in pepper and coffee trees. Nematode eggs and cuticles are the infection sites for biocontrol agents that penetrate those areas by the production of lytic enzymes. Paecilomyces variotii NV01 was isolated from pepper soil from the Dak Lak province. The purpose of this work is to study the activity of protease and chitinase in the strain Paecilomyces variotii NV01. The protease activity was determined to be 82.3 U/ml in a 0.
6p chauchaungayxua2 07-01-2020 30 0 Download
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The present study was conducted to evaluate the in vitro efficacy of native Trichoderma spp. against Pythium spp. and Rhizoctonia solani (Kuhn.) causing damping-off disease in Tomato. One hundred eighty soil samples were collected from 11 districts of Meghalaya and ninety seven Trichoderma isolates were obtained. Rapid screening against damping off pathogens (Pythium spp. and R. solani) of tomato revealed that 20 isolates showed maximum antagonism and subsequently 20 best isolates were selected for further analysis.
14p cothumenhmong1 08-12-2019 15 1 Download
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In an attempt to develop superior Trichoderma viride isolates for improvement in chitinase Enzyme productivity, induction of mutants was applied. After application of different concentrations of Ethyl methane sulfonate (EMS) and Hydroxyl amine (HA) treatments on T.viride mutants were obtained. Trichoderma viride was determined by its physiological state so that change in genetical conditions could alter the antagonism. Hence, the genetic modification using mutagenesis offers the potential for producing improved bioprotection is likely to enhance their biocontrol.
9p chauchaungayxua1 04-12-2019 19 1 Download
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Swollen chitin, flake chitin, powder chitin, and mushroom paste were used as substrates for chitinase production by Serratia marcescens GG5 in submerged fermentation. Enzyme production was 0.3 U/ml when the organism was grown in M9 medium supplemented with 0.5% swollen chitin and 0.5% soluble starch. Scanning electron microscopy revealed that Serratia marcescens GG5 digested the chitin flakes by producing chitinase.
6p vitamix 15-02-2019 20 2 Download
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Gram-negative Serratia marcescens is a potential biological control agent of fungal plant diseases in many tropical regions. Its antifungal activity is due to the production of prodigiosin, protease, chitinase, and other substances that act to weaken the fungal cell wall and cause lysis.
10p nutifooddau 27-01-2019 31 2 Download
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Insect pests and pathogens (fungi, bacteria and viruses) are responsible for severe crop losses. Insects feed directly on the plant tissues, while the pathogens lead to damage or death of the plant. Plants have evolved a certain degree of resistance through the production of defence compounds, which may be aproteic, e.g. antibiotics, alkaloids, terpenes, cyanogenic glucosides or proteic, e.g. chitinases,b-1,3-glu-canases, lectins, arcelins, vicilins, systemins and enzyme inhibitors.
16p research12 23-04-2013 42 1 Download
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The enzymatic properties of chitinase A fromVibrio carchariaehave been studied in detail by using combined HPLC and electrospray MS. This approach allowed the separation ofaandbanomers and the simultaneous monitoring of chitooligosaccharide products down to picomole levels. Chi-tinase A primarily generated b-anomeric products, indicating that it cata-lyzed hydrolysis through a retaining mechanism.
11p fptmusic 11-04-2013 27 1 Download
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Family 18 chitinases such as chitinase B (ChiB) fromSerratia marcescens catalyze glycoside hydrolysis via a mechanism involving the N-acetyl group of the sugar bound to the)1 subsite. We have studied the degradation of the soluble heteropolymer chitosan, to obtain further insight into catalysis in ChiB and to experimentally assess the proposed processive action of this enzyme. Degradation of chitosans with varying degrees of acetylation was monitored by following the size-distribution of oligomers, and oligomers were isolated and partly sequenced using 1 H-NMR spectroscopy....
12p awards 05-04-2013 52 4 Download