Chloroform method

The Anatolian water buffalo is a native water buffalo breed only being reared in Turkey. The objective of this study was to investigate gene polymorphisms on exons 4 and 5 of the growth hormone (GH) and on exon 10 of the growth hormone receptor (GHR), which are thought to be related to meat yields of Anatolian water buffalo. Blood samples of 192 water buffaloes from Sivas Province were used in DNA extractions by phenol-chloroform method. DNA samples were amplified by using specific primers in PCR.

6p dolomite36 30-12-2021 9 0   Download

The crude extract was obtained by the maceration method of dried power of Parmotrema praesorediosum lichen in methanol. This extract was then separated by the solid-phase extraction and eluted with petroleum ether, chloroform, ethyl acetate, acetone, and methanol in turn to give the corresponding extracts. Two compounds were isolated from chloroform extract by silica gel column chromatography.

6p spiritedaway36 28-11-2021 14 1   Download

In this study, five extracts and fractions of the aerial parts of Ludwigia hyssopifolia (G. Don) Exellincluding n-hexane, chloroform, ethyl acetate, methanol, and ethanol ones were screened for anti-H. pylori activity by disk diffusion method.

6p nguaconbaynhay11 07-04-2021 18 2   Download

Different methods available for extraction of plant genomic DNA suffers from one or more drawbacks including compromised quality, quantity and many more. The extraction of high-quality DNA Simarouba glauca DC is notoriously troublesome due to the high contents of polysaccharides and different secondary metabolites. Herein, we aimed to develop a modified CTAB extraction method to isolate DNA from tissues containing high levels of polysaccharides.

12p chauchaungayxua11 23-03-2021 15 2   Download

In the present investigation a total of 100 faecal samples/rectal swabs were collected from dogs suffering with gastrointestinal disorders and showing the symptoms of canine parvovirus, like bloody diarrhoea, vomiting and pyrexia. The samples were tested initially with SYBR green dye based real time PCR with different combinations of the cycles and the results obtained were further confirmed by conventional PCR. We could able to standardize the real time PCR which was more helpful in screening of the faecal samples further in large numbers.

7p trinhthamhodang9 16-12-2020 16 1   Download

The insecticidal and repellent activities were observed by residual film method and surface film application methods respectively. Leaf, stem and root of Mimosa pudica L. were screened through Petroleum ether, chloroform, ethyl acetate and methanol extracts against flat grain beetle, Cryptolestes pusillus (Schon.).

14p trinhthamhodang9 16-12-2020 13 2   Download

The experiment was conducted to assess Microbial biomass carbon and Nitrogen on 84 days organic and conventional incubated soils in Nadia district of West Bengal. Soil microbial biomass carbon & nitrogen is determined by Chloroform Fumigation Extraction method (FE) followed by Kjeldal Digestion method. The results depicted that, microbial biomass carbon and nitrogen was more in organic soil of Phulia and Gayeshpur district rather than conventional method.

4p caygaocaolon6 30-07-2020 11 1   Download

A simple and easy protocol for isolation of DNA from major plant pathogen Alternaria spp has been developed in which DNA extracted using CTAB extraction method from fresh mycelium of fungi crushed with sterile sand particles and liquid nitrogen. The DNA was precipitated first with equal volume of phenolchloroform-isoamylalcohol (25:24:1) and further with equal volume of chloroform-isoamylalcohol (24:1). Repeated centrifugation was done at 10000 g for 10 minutes at room temperature.

4p angicungduoc6 22-07-2020 11 1   Download

Antibacterial activities of Black pepper fruit extract were studied against some bacterial strains as- Pseudomonas fluorescens, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus vulgaris, and Bacillus subtilis. The black pepper fruit extracts were prepared in four organic solvents as methanol, ethyl acetate, chloroform and benzene. Effectiveness against bacterial strains showed by all extract (which prepared by organic solvent) but the benzene extract showed the maximum growth inhibition 81.20% against Klebsiella pneumoniae bacteria at 100µl/ml extract concentration.

6p chauchaungayxua6 26-06-2020 16 0   Download

The present study was conducted to asses phytochemical and antibacterial activity of various crude extracts viz, pet-ether, chloroform, methanol and aqueous extracts of Salvia aegyptiaca against bacteria and fungi such as Staphylococcus aureus (MTCC Code - 9886), Pseudomonas aeruginosa (MTCC Code - 6458), Aspergillus niger (MTCC Code - 872), Aspergillus flavus (MTCC Code - 8790) by adapting agar well diffusion method. The highest percentage of extraction yield was observed in aqueous extract followed by methanol, chloroform and petether extracts.

9p nguaconbaynhay6 23-06-2020 32 1   Download

For many crucial experiments in gene cloning and further identification, purified and high quality genomic DNA is the prerequisite. In plants, polysaccharides and secondary metabolites impurities removal is complicated and time-consuming protocols are available for pigeon pea plant. In present study, rapid and proficient method for DNA extraction was optimized. The method utilizes small amount of plant material to lessen inhibitory agents like polysaccharides and phenolic compound.

4p quenchua6 15-06-2020 12 1   Download

The present study was aimed to evaluate the bioactive phytochemical constituents of Adina cordifolia leaves, with this broad objectives the total phenolic contents (TPC), total flavonoid contents (TFC), total antioxidant potential and GC-MS analysis of leaf extracts were carried out. For the identification of medicinally important compounds in Adina cordifolia leaf, extracts were prepared using cold extraction method in different solvents of varying polarities viz., chloroform, ethyl acetate, acetone and methanol.

13p gaocaolon5 14-06-2020 7 1   Download

The present study was designed to evaluate in vitro antimicrobial activity and antioxidant activity and anticancer activity of different five extracts of Euphorbia tirucalli Stem. Plant roots were extracted in different five solvents viz. Hexane, Chloroform, Methanol, Water and Methanol: Water (90:10) through Soxtherm according to polarity gradients. Aiming to investigate antimicrobial activities, agar well diffusion method was followed using five pathogenic bacteria and five fungi as test organisms.

9p kethamoi4 18-04-2020 10 1   Download

Phytochemical research on the chloroform and ethyl acetate extracts of the rhizomes of Curculigoannamitica Gagnep. collected in Binh Chau - Phuoc Buu Nature Reserve, Ba Ria - Vung Tau province, led to the isolation of five phenolic compounds namely 3,4- dimethoxysalicylicacid(1), 2-hydroxy-6-methoxybenzoicacid(2), 4-hydroxybenzoic acid (3), pcoumaric acid (4) and 2-(ethoxymethyl) benzene-1,4-diol (5). Their chemical structures were confirmed by spectroscopic methods including HR-ESI-MS, ESI-MS,1D and 2D-NMR and compared with reported data.

7p abcxyz123_02 03-03-2020 17 1   Download

Molecular Biology experiments including gene cloning, restriction digestion, identification by 18S r-DNA requires purified and high quality genomic DNA. The major problems in DNA isolation from fungus are impurities such as polysaccharides, protein and RNAs which interfere in PCR reaction. In this study, proficient method for DNA extraction without using liquid nitrogen, CTAB or lysozyme was optimized. The method utilizes very few chemical compounds.

4p nguathienthan2 19-12-2019 18 0   Download

Microorganisms play a crucial role in living organisms. Some microorganisms are beneficial for us some are pathogenic. The aim of the study is to evaluate the activity of the fruits of Artocarpus lakoocha Roxb. against certain pathogenic microbial strains. Petroleum ether, chloroform and methanol extract of these fruits were used by disk diffusion method on Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Salmonella typhi, Bacillus cereus, Salmonella paratyphi and two fungal strains. All the fruit extracts showed the antimicrobial activity against all the microorganisms.

7p nguaconbaynhay1 08-12-2019 12 1   Download

The extraction of genomic DNA from microbial cells plays a significant role in PCR-based applications such as molecular diagnosis, microbial taxonomy, screening of genetically engineered microorganisms, and other such PCRbased applications. Currently, many methods for extraction of genomic DNA from microorganisms have been developed. However, these methods either require hazardous chemicals or consist of time-consuming steps for effective execution.

9p caygaocaolon1 13-11-2019 17 1   Download

The antibacterial activities of the alcohol, ethyl acetate, acetone and chloroform extracts of 5 plant species were studied. The extracts of Pimpinella anisum (L.) (anise, aniseed) (seed), Coriandrum sativum (L.) (coriander, cilantro) (seed), Glycyrrhiza glabra (L.) (liquorice) (root), Cinnamomum cassia Blume (cassia bark, Chinese cinnamon) (bark), and Juniperus oxycedrus (L.) (juniper) (seed) were tested in vitro against 13 bacterial species and strains by the agar diffusion method.

6p vitamix 15-02-2019 13 0   Download

The aim of the present study was to evaluate antimicrobial susceptibilities of enterococci isolated from water, soil, waste, and food samples to determine resistance mechanisms and their transferability, and to test clonality of these environmental enterococci.

6p sony2711 01-02-2019 18 1   Download

The aim of this study was to determine phylogenetic relationships of 6 native cattle breeds of Turkey using 7 Y chromosomespecific microsatellite DNA markers. DNA samples were isolated from Anatolian Black, Anatolian Grey, South Anatolian Red, Native Southern Anatolian Yellow, East Anatolian Red, and Zavot cattle using a standard phenol/chloroform method.

8p sony2711 01-02-2019 23 1   Download