Co-immunoprecipitation
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ATP-sensitive K + channels are an octameric assembly of two proteins, a sulfonylurea receptor (SUR1)and an ion conducting subunit (Kir 6.0). We have examined the role of the C-terminus of SUR1 by expressing a series of truncation mutants together with Kir6.2 stably in HEK293 cells. Bio-chemical analyses using coimmunoprecipitation indicate that SUR1 deletionmutants andKir6.2 assemble and that a SUR1 deletion mutant binds glibenclamide with high affin-ity.
11p tumor12 22-04-2013 41 3 Download
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A wide range of approaches has been applied to examine the quaternary structure of G protein-coupled receptors, the basis of such protein–protein interactions and how such interactions might modulate the pharmacology and function of these receptors. These include coimmunoprecipitation, var-ious adaptations of resonance energy transfer techniques, functional com-plementation studies and the analysis of ligand-binding data.
12p fptmusic 11-04-2013 45 1 Download
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The voltage-gated potassium channel Kv4.3 was coexpressed with itsb-sub-unit Kv channel-interacting protein 2 and the angiotensin type 1 receptor in HEK-293 cells. Proteomic analysis of proteins coimmunoprecipitated with Kv4.3 revealed that Kv4.3 is associated with Rap guanine nucleotide exchange factors MR-GEF and EPAC-1.
10p media19 04-03-2013 34 1 Download