Coat protein gene
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The induced resistance was tested using I-ELISA and immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) for the detection of CMV coat protein gene (cp), which proved that the mentioned symptomless plants were virus-free or with a low level of virus infection.
7p schindler 30-12-2021 11 0 Download
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The identity of the causal virus was established on the basis of symptomatology and molecular assays. Primers designed against coat protein (CP) gene resulted in obtaining a desired amplicon of ~162 bp confirming the isolate to be of Cucumber mosaic virus
7p chauchaungayxua10 19-03-2021 16 1 Download
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Banana (Musa paradisiaca) is herbaceous plant belonging to the family Musaceae of the genus Musa and is native to the tropical region of South East Asia. Viral diseases have been causing considerable damage to banana production in banana growing areas due to their systemic invasion and common spread through vegetatively propagated planting materials.
6p trinhthamhodang1218 26-02-2021 11 2 Download
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Bunchy top disease caused by the banana bunchy top virus (BBTV) is a serious disease in hill banana. Detection of the BBTV infection in the planting material could help in the effective management of the disease. Primers were designed for the PCR amplification of BBTV coat protein (CP). In the present study, to confirm the virus infection and to quantify the viral load in the plants, samples from infected and healthy banana were subjected to PCR and southern blot hybridization.
14p trinhthamhodang1215 23-09-2020 9 1 Download
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The present study was conducted to characterize Leaf Curl Virus infecting tomato in south Gujarat region showing typical leaf curl symptom with upward/downward curling along with vein clearing of the leaves. A part of DNA-A molecule of ~1200 bp was amplified with a Begomovirus specific primers confirming it to be Begomovirus. On sequencing, a 1125 bp nucleotide sequence (Accession no- KU921251) was obtained. Amplified fragment had two genes viz., virus coat protein (V1) gene and pre coat protein (V2) gene.
9p chauchaungayxua6 26-06-2020 11 0 Download
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Mungbean (Vigna radiata (L.) Wilczek) is a fast-growing warm-season legume, native to the Indian subcontinent; widely cultivated in Southeast Asia. It is an important pulse crop because of its short growth duration, low water requirement, and as a crop suitable for crop rotation and nutritional security being a rich source of protein. Biotic stress produced by viruses especially yellow mosaic virus (YMV) is the main constraint in its production causing substantial yield losses. The detection and diagnosis of viruses is most important to excogitate a management strategy.
18p angicungduoc5 14-06-2020 15 0 Download
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Yellow mosaic virus is the most destructive disease of urdbean causing 5-100 per cent yield loss. BGYMV belongs to genus Begomovirus of the family Geminiviridae transmitted by white fly (Bemisia tabaci Gennadius). Polymerase chain reaction of yellow mosaic virus infecting blackgram samples using MYMV-CP-F/MYMV-CP-R primers amplified the expected product of size 1000 bp from blackgram infected samples. Expected PCR products of size 1000 bp obtained were cloned, sequenced and assembled.
11p trinhthamhodang5 16-05-2020 20 1 Download
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Tomato leaf curl virus (ToLCV) is a major Geminivirus which cause serious loss to tomato production in tropical and subtropical regions of the world. Hence, considered as major constraint to tomato cultivation. In the present study ToLCV coat protein gene was cloned and expressed using recombinant DNA technology approach. The ToLCV infected tomato leaf samples were collected from tomato fields near Krishi Vigyan Kendra, Dharwad. Further, the total DNA from ToLCV infected tomato leaf sample was isolated by following CTAB protocol.
11p nguathienthan4 18-04-2020 11 2 Download
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Chilli (Capsicum annuum L.) is an important spice crop severely affected by the virus causes leaf curl symptoms. Efficient transmission of Chilli Leaf Curl Virus was done by using a standardized buffer for sap inoculation to chilli plants. In present investigation, we have used three simple methods i.e. inoculation by syringe, rubbing and by immersion (dip) for sap inoculation and found reproducible when the sap from the infected plants was inoculated onto healthy plants. Out of the three methods used, syringe inoculation was found most efficient method for sap transmission.
7p kethamoi4 16-04-2020 9 2 Download
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Ridge gourd yellow mosaic disease (RgYMD) caused by the strain of Tomato leaf curl New Delhi virus (ToLCNDV) is an emerging disease in India. Early detection of ToLCNDV in ridge gourd has great significance in the management of the disease. An innovative loop-mediated isothermal amplification (LAMP) assay was standardized for rapid detection of ToLCNDV. Assay was carried out using the set of six primers (F3, B3, FIP, BIP, LF and BF) specific to the coat protein (CP) gene of the virus.
14p caygaocaolon3 09-03-2020 11 0 Download
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Tomato leaf curl virus disease (ToLCVD) is one of the major causes for highest economical loss of tomato in India. In this study, Natural incidence of ToLCV disease on tomato was observed at Gujarat, India in the March, 2019. The infected plants revealed systemic viral symptoms in the form of sever leaf curling, leaf crumple with marginal yellowing, stem warped and stunted. Molecular methods were followed to further characterized coat protein (CP) gene of tomato leaf curl virus using specified primers.
11p trinhthamhodang3 12-02-2020 13 1 Download
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Horse gram leaf samples showing yellow mosaic symptoms gave positive results with HgYMV specific primer pairs (HgYMV-CP-F/HgYMV-CP-R) and yielded amplicons of ~1000 bp whereas no PCR product was obtained from DNA extracted from healthy samples in PCR. The 1000 bp PCR products were cloned, sequenced, assembled and its length was determined as 885 bp including 111 bp pre-coat protein at 5’ end and 774 core coat protein. The length of the predicted amino acid sequence was found to be 257.
11p nguaconbaynhay3 07-02-2020 15 0 Download
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The present study was carried out to know the sap transmission nature of sunflower necrosis virus and detection by serological and molecular means. The disease was successfully transmitted through mechanical sap using 0.05 M potassium phosphate buffer. A maximum transmission of 52.33 per cent on cultivar KBSH-44, 51.33 per cent on KBSH-1, and 48.33 per cent on Morden. Under artificial inoculation, the newly emerged leaves showed slight downward curling, puckering and chlorosis followed by necrosis.
6p cothumenhmong1 11-12-2019 13 1 Download
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Banana bract mosaic disease (BBrMD) is one of the most important viral diseases of banana which leads to a yield reduction. We have identified banana bract mosaic virus (BBrMV) in banana plants growing in the regions of southern India based on symptomatology, sequence homology, and Serodiagnostic assays. The viral sequence encoding the coat protein was specifically amplified by RT-PCR (Reverse Transcriptase – Polymerase chain reaction) using specific primers bordering the Coat Protein (CP) gene.
12p cothumenhmong1 10-12-2019 16 0 Download
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The coat protein (CP) genes of the genomic RNA of 2 severe Turkish isolates of Zucchini yellow mosaic virus (ZYMV) from squash and muskmelon [ZYMV-Adana (Ad) and ZYMV-Ahlat (Ah), respectively] were cloned, and their complete nucleotide sequences and deduced amino acids were determined.
7p vivant2711 01-02-2019 23 2 Download
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Fifteen different biologically important citrus tristeza virus (CTV) isolates (B14, B15, B23, B31, B52, B57, T36, D9, D10, D11, D12, D15, D16, D20 and D32) were tested using strain-specific biotin-labeled oligonucleotide probes.
11p aquaman27 15-01-2019 15 1 Download
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Protein and RNA-mediated forms of pathogen-derived resistance (PDR) have been developed against many viruses in different plants. However, no resistance has been reported against Citrus tristeza virus (CTV), a closterovirus, in Citrus species transformed with coat protein genes or other sequences of CTV.
10p aquaman27 15-01-2019 20 1 Download
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Tritium planigraphy study of structural alterations in the coat protein of Potato virus X induced by binding of its triple gene block 1 protein to virions Elena Lukashina1, Gennady Badun2, Natalia Fedorova1, Alexander Ksenofontov1, Maria Nemykh1, Marina Serebryakova3, Anna Mukhamedzhanova4, Olga Karpova4, Nina Rodionova4, Lyudmila Baratova1 and Evgeny Dobrov1 1 2 3 4 A.N.
10p viettel02 20-02-2013 99 3 Download
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Ever since their discovery adenoviruses have proven to be a tremendous asset to biologists. Through the study of the adenoviruses, we have learned not only about the virus structures, mechanisms of viral replication, but also about eukaryotic gene expression, alternative splicing, regulation of cell cycle progression, and apoptosis. In the last five years, there has been an explosion in the use of adenoviruses as vectors for gene transfer to a variety of mammalian cells.
334p thuytienvang_1 31-10-2012 39 3 Download