![](images/graphics/blank.gif)
Conditional knock-out
-
Part 2 book "Transgenic mouse - Methods and protocols" includes content: Introduction - Engineering BAC reporter gene constructs for mouse transgenesis; targeting vector construction through recombineering; generating conditional knockout mice; hypomorphic mice; micer targeting vectors for manipulating the mouse genome; knock in approaches; generation of a series of knock in alleles using rmce in es cells; selection of targeted mutants from a library of randomly mutagenized ES cells.... and other contents.
190p
oursky09
12-11-2023
8
3
Download
-
Part 1 book "Mouse genetics - Methods and protocols" includes content: Generation of conditional knockout mice, generating mouse lines for lineage tracing and knockout studies, generating chimeric mice from embryonic stem cells via vial coculturing or hypertonic microinjection, genetic inducible fate mapping in adult mice using tamoxifen dependent cre recombinases,.... and other contents
259p
oursky06
17-10-2023
4
2
Download
-
Part 2 book "Transgenic mouse, methods and protocols" includes content: Engineering BAC reporter gene constructs for mouse transgenesis; targeting vector construction through recombineering, generating conditional knockout mice, hypomorphic mice, knock in approaches, selection of targeted mutants from a library of randomly mutagenized ES cells,...and other contents.
190p
oursky05
20-09-2023
2
2
Download
-
Nonclustered mouse protocadherin genes (Pcdh) encode proteins with a typical single ectodomain and a cytoplasmic domain with conserved motifs completely different from those of classic cadherins.
33p
vikolindagrabar
27-07-2022
2
2
Download
-
Conditional knockout mice and transgenic mice expressing recombinases, reporters, and inducible transcriptional activators are key for many genetic studies and comprise over 90% of mouse models created. Conditional knockout mice are generated using labor-intensive methods of homologous recombination in embryonic stem cells and are available for only ~25% of all mouse genes.
15p
vialfrednobel
29-01-2022
14
0
Download
-
CRISPR/Cas9 enables the targeting of genes in zygotes; however, efficient approaches to create loxPflanked (floxed) alleles remain elusive. We show that the electroporation of Cas9, two gRNAs, and long single-stranded DNA (lssDNA) into zygotes, termed CLICK (CRISPR with lssDNA inducing conditional knockout alleles), enables the quick generation of floxed alleles in mice and rats.
8p
vibeauty
23-10-2021
6
0
Download
-
Environmental stresses including abiotic stresses and biotic stresses limit yield of plants. Stresstolerant breeding is an efficient way to improve plant yield under stress conditions. Genome editing by CRISPR/ Cas9 can be used in molecular breeding to improve agronomic traits in crops, but in most cases, with fitness costs.
15p
vijichea2711
28-05-2021
5
1
Download
-
Nuclear Factor-Kappa B (NF-kB) is a family of transcription factors that are important in embryonic development, inflammation, epithelial-to-mesenchymal transition and cancer. The 65 kDa RelA subunit is the major transcriptional activator of the NF-kB pathways.
10p
viwyoming2711
16-12-2020
9
0
Download
-
Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes...
11p
thulanh19
05-11-2011
41
1
Download
CHỦ ĐỀ BẠN MUỐN TÌM
![](images/graphics/blank.gif)