Conventional staining techniques

The study showed that the immunochromatography assay tests were reliable and easy to use. Blood samples were collected from 175 patients presenting with typical features suggesting clinical malaria. These blood samples were subjected to various test which included thin and thick smears stained with JSB, Giemsa, Leishman, Fields stain and the other methods employed which included Arcidine orange staining, Quantitative Buffy Coat methods, immunochromatography strips – optimal and para HIT – f. Assay are used. Gene amplification technique (Polymerase chain reaction) was used.

15p nguathienthan8 20-10-2020 17 2   Download

Studies have been targeting to evaluate newer automated culture systems techniques for different types of samples to provide a more rapid diagnosis relying on sensitive and specific principle with the ability to discriminate between viable and dead mycobacterial bacilli. This study aimed at assessment of bactalert, biomerieux ® culture system as a rapid diagnostic tool for tuberculous pleural effusion fluid in comparison with Lowenstein Jensen conventional culture and Zeil Nelseen stained smears.

5p nguaconbaynhay6 24-06-2020 10 0   Download

The cytogenetic study was conducted on total 30 breeding bucks of Osmanabadi and Boer breed (15 of each) to find out chromosomal abnormalities and to study QFQ- banding pattern of the chromosomes. Short term lymphocyte culture technique was used for the display chromosomal metaphases. The few slides were conventionally stained with 4 per cent giemsa stain and some were subjected for QFQ banding. The modal diploid chromosome number was 2n=60 for breeding bucks of Osmanabadi and Boer breed of goat.

5p chauchaungayxua5 05-05-2020 8 1   Download

Aim of the study is to detect Cryptosporidium, a newly emerging parasite in malnourished children with and without diarrhea, using conventional methods and special staining techniques. A total of 727 children comprised of 497 malnourished and 230 normal children having with and without diarrhoea. Two consecutive samples were collected from each individual and one preserved using 10% formalin. Examination of helminthes and protozoan in the stool samples were done by direct wet mount method and by formalin ether sedimentation method.

8p nguathienthan4 21-04-2020 14 2   Download

Bacterial infections are most commonly encountered by clinicians in developing countries. Area-specific monitoring studies done to assess the type of pathogens and their antibiotic sensitivity patterns which help the clinician to opt for best empirical treatment. The hospital based cross sectional study is conducted on various samples collected using sterile techniques from 2474 clinically-suspected cases attending RDBP, Jaipuriya Hospital from Jan 2016 to Dec 2017 and tested bacteriologically using standard conventional procedures.

9p chauchaungayxua3 07-02-2020 24 1   Download

The present study was aimed to do comparative evaluation of efficacy of various staining techniques and rapid diagnostic tests used for identifying the Plasmodium falciparum parasite, to determine sensitivity and specificity of rapid diagnostic methods and compare the results of rapid diagnostic methods with conventional microscopy. Total of 107 male and female febrile patients of all age, clinically suspected to be positive for malaria, willing to participate and to sign the informed consent form were included in this study.

9p cothumenhmong1 10-12-2019 19 0   Download

Sarcocystis species are considered as one of the most prevalent parasites of bovines. Sarcocysts occur either macroscopically or microscopically in the tissues of bovine intermediate hosts. Sarcocystosis being an occult infection, most of the time diagnosis in intermediate host by clinical symptoms is unreliable. In this study, the macrosarcocysts were observed in the infected muscle sample by naked eye examination.

7p cothumenhmong1 08-12-2019 13 1   Download

The highly infectious nature of tuberculosis (TB) urges the need to increase the efficiency and rapidity of lab methods. The coexistence of TB and cancer was investigated using Mycobacterium tuberculosis direct test (MTD) and the mycobacteria growth indicator tube (MGIT), besides the conventional lab procedures. All assays were performed on 101 patients clinically suspected of having a mycobacterial infection.

6p kethamoi1 20-11-2019 12 1   Download

Water-borne diseases have been estimated to cause more than two million deaths and four billion cases of diarrhea annually. Water-borne pathogenic organisms include bacteria, protozoa, and viruses. Heavy metal contamination of water is also a potential threat to human health. This study aimed to detect contamination of potable water with protozoal and bacterial pathogens as well as heavy metals in Gharbiya governorate in the middle of the Nile Delta, Egypt. Therefore, this study was conducted on water samples from 3 different localities in Gharbiya governorate throughout the year 2014.

14p kequaidan1 16-11-2019 39 1   Download

The diagnosis of pulmonary tuberculosis can be made by the detection of acid fast bacilli by direct microscopy, using carbol fuchsin stain and/or fluorochrome stain. Microscopy is a rapid method but lacks sufficient sensitivity and does not distinguish between different species of mycobacteria. The sensitivity of microscopy is often not more than 25-40% as compared to culture, but under ideal conditions, it is possible to achieve a rate of 60-70%.

4p taisaocothedung 12-01-2013 51 3   Download

The goals of experimental neuroscience research are fundamentally to gain mechanistic understanding of the pathology of disease in order to identify appropriate targets for potential pharmacological intervention and evaluation of putative therapies. With the advances in gene manipulation and transgene technologies, we have unprecedented ability to generate animal models of disease that more closely mimic the clinical conditions. Noninvasive techniques capable of investigating altered pathophysiologies are now of paramount importance.

366p echbuon 02-11-2012 59 5   Download

Bacteria are non-chlorophyllated unicellular organisms that reproduce by fission and do not present nuclear envelope. Gram´s stain is a staining technique used to classify bacteria based on the different characteristic of their cell walls. Gram-positive or Gram-negative bacteria are determined by the amount and location of peptidoglycan in the cell wall, exhibiting different chemical compositions and structures, cell-wall permeabilities, physiologies, metabolisms, and pathogenicities.

355p yeutinh98 22-09-2012 82 15   Download