Correctly identifying genomic
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Single nucleotide polymorphisms (SNPs) which capture a significant impact on a trait can be identified with genome-wide association studies. High linkage disequilibrium (LD) among SNPs makes it difficult to identify causative variants correctly.
14p vihagrid 30-01-2023 7 3 Download
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To fully utilize the power of single-cell RNA sequencing (scRNA-seq) technologies for identifying cell lineages and bona fide transcriptional signals, it is necessary to combine data from multiple experiments. We present BERMUDA (Batch Effect ReMoval Using Deep Autoencoders), a novel transfer-learning-based method for batch effect correction in scRNA-seq data.
15p vielonmusk 30-01-2022 13 0 Download
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The incorporation of unique molecular identifiers (UMIs) in single-cell RNA-seq assays makes possible the identification of duplicated molecules, thereby facilitating the counting of distinct molecules from sequenced reads.
18p viarchimedes 26-01-2022 7 0 Download
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Millions of nucleotide variants are identified through cancer genome sequencing and it is clinically important to identify the pathogenic variants among them. By introducing base substitutions at guide RNA target regions in the genome, CRISPR-Cas9-based base editors provide the possibility for evaluating a large number of variants in their genomic context.
25p viarchimedes 26-01-2022 11 0 Download
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Cell-penetrating peptides (CPPs) are short peptides (5–30 amino acids) that can enter almost any cell without significant damage. On account of their high delivery efficiency, CPPs are promising candidates for gene therapy and cancer treatment. Accordingly, techniques that correctly predict CPPs are anticipated to accelerate CPP applications in future therapeutics. Recently, computational methods have been reportedly successful in predicting CPPs. Unfortunately, the predictive performance of existing methods is not satisfactory and reliable so as to accurately identify CPPs.
11p vilarryellison 29-10-2021 7 0 Download
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Transcriptomic profiles can improve our understanding of the phenotypic molecular basis of biological research, and many statistical methods have been proposed to identify differentially expressed genes (DEGs) under two or more conditions with RNA-seq data.
14p visilicon2711 20-08-2021 19 2 Download
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Aging is a multifactorial process that affects multiple tissues and is characterized by changes in homeostasis over time, leading to increased morbidity. Whole blood gene expression signatures have been associated with aging and have been used to gain information on its biological mechanisms, which are still not fully understood.
12p vilichoo2711 23-06-2021 11 1 Download
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Identifying protein-coding genes from species without a reference genome sequence can be complicated by the presence of sequencing errors, particularly insertions and deletions. A number of tools capable of correcting erroneous frame-shifts within assembled transcripts are available but often do not report back DNA sequences required for subsequent phylogenetic analysis.
11p vioklahoma2711 19-11-2020 15 3 Download
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Correctly identifying genomic regions enriched with histone modifications and transcription factors is key to understanding their regulatory and developmental roles. Conceptually, these regions are divided into two categories, narrow peaks and broad domains, and different algorithms are used to identify each one.
10p vioklahoma2711 19-11-2020 9 2 Download
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Present study was conducted with the objectives to identify single nucleotide polymorphism in sperm associated antigen 11 B(SPAG11B) gene and to analyze association between identified polymorphism with conception rate in Murrah bulls in ICAR-National Dairy Research Institute (NDRI) herd, Karnal. A 373 base pair region covering partial intron 2, exon 3 and partial intron3 of bovine SPAG11 gene was amplified using genomic DNA extracted from eighty six Murrah bulls and genotyped using sequencing and polymerase chain reaction- restriction fragment length polymorphisms (PCR-RFLP) methods.
7p cothumenhmong3 22-02-2020 19 0 Download
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Ten incorrect diagrams were created by randomly changing the origin or destination of one relationship per diagram. The layouts of the incorrect diagrams were visually comparable to those of the correct diagrams: as we did not intend to analyse the responses to the incorrect diagrams, their layout was not important.
5p giamdocamnhac 06-04-2013 37 2 Download
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Two cell lines were created; Flp293A and 293 FLEX, both derived from 293 cells. The former pseudotyped with amphotropic and the latter with GaLV envelopes. Recently, a PG13-based murine producer cell line was also established using this strategy (Loew et al. 2009). A favorable chromosomal site for stable and high retroviral vector production is first identified and tagged.
0p kmkmkmkmkm 08-09-2012 55 4 Download
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Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Correction: DNA microarray analysis of rheumatoid arthritis susceptibility genes identified by genome-wide association studies...
1p thulanh16 21-10-2011 48 3 Download