Cry1Ac resistant
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Insect bioassays were carried out of field strain of Helicoverpa armigera that survived on Bt cotton. These insects were reared on Cry1Ac incorporated diet that served as the initial culture for selection of resistance. Based on the partial sequences of putative trypsin mRNA of Helicoverpa armigera (accession no. AFO45138) trypsin specific primers were designed.
7p chauchaungayxua12 29-07-2021 13 1 Download
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Sugarcane (Saccharum spp. hybrids) is considered the most globally important sugar-producing crop and raw material for biofuel. Insect attack is a major issue in sugarcane cultivation, resulting in yield losses and sucrose content reductions.
14p viharuno2711 24-04-2020 9 1 Download
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We reportedpreviously a direct correlationbetween reduced soybean agglutinin binding to 63- and 68-kDa midgut gly-coproteins and resistance to Cry1Ac toxin from Bacillus thuringiensisin the tobacco budworm (Heliothis virescens). In the present work we describe the identification of the 68-kDaglycoproteinas amembrane-bound formof alkaline phosphatase we term HvALP. Lectin blot analysis of HvALP revealed the existence of N-linked oligosaccharides containing terminal N-acetylgalactosamine required for [ 125 I]Cry1Ac binding in ligand blots. ...
9p awards 05-04-2013 44 2 Download