Báo cáo khoa học: Characterization of a Cry1Ac-receptor alkaline phosphatase in susceptible and resistant Heliothis virescens larvae
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We reportedpreviously a direct correlationbetween reduced soybean agglutinin binding to 63- and 68-kDa midgut gly-coproteins and resistance to Cry1Ac toxin from Bacillus thuringiensisin the tobacco budworm (Heliothis virescens). In the present work we describe the identification of the 68-kDaglycoproteinas amembrane-bound formof alkaline phosphatase we term HvALP. Lectin blot analysis of HvALP revealed the existence of N-linked oligosaccharides containing terminal N-acetylgalactosamine required for [ 125 I]Cry1Ac binding in ligand blots. ...
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