Culture and biochemical characteristics

Ebook "Disposable bioreactors (Advances in biochemical engineering/biotechnology, Volume 115)" includes eight contributions from renowned experts, is to give an up-to-date overview of the main disposable bioreactors, their working principles, characteristics, known engineering aspects, and potential applications. R. Brecht discusses disposable bioreactor technology development in pharmaceutical glycoprotein manufacturing from the view of the applier (CMO).

228p manmanthanhla0201 26-02-2024 7 1   Download

The bacteria capable of fixing atmospheric nitrogen were isolated from cassava cultivated soils of Vietnam. The potential isolates were identified by analyzing the 16S rRNA gene and by morphological, biochemical, cultural characteristics. The selected isolates were assigned to the species Bacillus sp. DQT2 M17, Bacillus subtilis DTAN6 M17, and Bacillus megaterium DSHB I8.

9p vicaptainmarvel 21-04-2023 5 2   Download

Klebsiella is an opportunistic bacterial pathogen of man and animals known for presence of diversity of Anti Microbial Resistance (AMR). The present study was undertaken to isolate and study the anti-microbial resistance in Klebsiella isolates from cattle and buffaloes. Sixty (60) samples, comprising of 33 mastitic milk and 27 diarrhoeic faecal samples were collected aseptically from cattle and buffaloes. From these 60 samples, 11 (18.33%) Klebsiella species were isolated and identified on the basis of cultural, morphological and biochemical characteristics.

5p caygaocaolon11 21-04-2021 10 2   Download

In the present study, faecal and blood samples were collected, aseptically, from an elephant arrived to the Veterinary Clinics of the College. Immediately, samples were used for bacterial isolation. A bacterial isolate, isolated from the faecal sample of an elephant was identified at the genus level to be of Escherichia species by adopting Enterobacteriaceae identification kit used for studying biochemical characteristics and carbohydrate utilization tests (Gram negative bacilli showing mainly ONPG positive, oxidase negative and nitrate reduction positive).

7p chauchaungayxua11 23-03-2021 15 2   Download

The present study was conducted to determine and to compare the microbial population of ATM and computer keyboard within the University of Port Harcourt and also to determine the role of plasmid and resistance in Staphylococcus sp isolated from the high touch areas. A total of 70 swabbed samples was collected and cultured on different media and the organisms were identified using its colonial, morphological biochemical characteristics. The identified bacteria were subjected to antibiotic sensitivity test using the disc diffusion method.

8p trinhthamhodang1218 26-02-2021 14 2   Download

Rhizome rot or tip over disease of banana has been occurring in serious form and affecting the yield wherever bananas are being cultivated. The morphological characteristics of the eighteen isolates of the bacterium revealed that cells were rod shaped, occurred singly, Gram negative and peritrichously flagellated. The pathogen was identified on the basis of cultural, morphological and biochemical characteristics and its pathogenicity in banana was proved using Koch‟s postulates.

6p gaocaolon9 22-12-2020 13 2   Download

Recombinant human CD45RO antigen was expressed from E. coli BL21 (DE3), purified and analyzed by SDS-PAGE and Western blotting. The purified CD45RO antigen was used to immunize Balb/c mice. Spleen cells from immunized mouse were collected and fused with P3X63Ag8.653 myeloma cells to form hybridoma. Anti-CD45 antibody-secreting capacity of hybridoma clones was evaluated by ELISA assay. Anti-CD45 MAb from the culture supernatant of the chosen hybridoma clone was purified by affinity chromatography. The MAb was characterized the biochemical characteristics and biological activity.

8p trinhthamhodang9 04-12-2020 12 2   Download

A total of 10 samples were collected from Agricultural waste dump sites from various areas of Ladakh and 12 isolates of bacteria were isolated using Nutrient Agar medium. The optimal cultural conditions, microbiological characteristics, biochemical characteristics, antagonistic and synergistic activities within the strains and production of extracellular enzymes of the bacterial strains were documented. Colonies were isolated, cultured and characterized by gram staining and biochemical tests. Six isolates were found to be gram negative while 4 were gram positive.

7p kethamoi5 03-06-2020 14 0   Download

The present study was taken up to evaluate the antibiogram against different mastitogenic pathogens isolated from the milk samples of clinical bovine mastitis cases. A total of 78 milk samples were collected from different dairy farmers of Nialiblock, Odisha and processed in Animal Disease Research Institute (ADRI), Phulnakhara, Cuttack for isolation, identification and antibiogram of pathogens. The samples were screened for mastitis by modified California mastitis test (MCMT). The milk samples were inoculated in BHI broth and incubated for 24-48 hours.

6p trinhthamhodang1213 30-05-2020 16 1   Download

The nine isolates of Rhizobium meliloti were isolated on CR-YEMA medium from root nodules and rhizosphere soil of fenugreek. Four isolates showed absorption of Congo red and rest five isolates did not absorbed Congo red. All the isolates showed similar cultural characteristic for colony colour and shape except for colony size.

8p chauchaungayxua5 08-05-2020 12 1   Download

The present investigation was carried out with an aim to isolate and characterize native Bacillus thuringiensis from Anand district of Middle Gujarat. Total 67 probable B. thuringiensis were isolated from 17 diverse soil samples, out of which 9 isolates were selected based on presence of parasporal crystal body and primary toxicity test. Selected nine native isolates were characterized based on their morpho-cultural and biochemical characteristics.

10p quenchua4 16-04-2020 7 0   Download

Mastitis is the most economically significant disease of dairy animals. This condition is associated with a significant reduction in milk yield, increased costs of production and deteriorated milk quality. In the present study a total number of 40 bovine mastitis milk samples were collected to isolate and identify the Pseudomonas aeruginosa. All the samples were inoculated into nutrient broth which was further cultured on agar plates. Colonies showing typical Pseudomonas morphological characteristics were processed for biochemical analysis.

6p chauchaungayxua4 18-03-2020 13 0   Download

Lactic acid bacteria are described as heterogeneous group of regular, gram-positive, rod shaped, non-motile, non-spore forming bacteria with absence of catalase enzyme. Laboratory study was undertaken to isolate and characterize the lactic acid bacteria from silkworm gut in phenotype basis. A total of 21 silkworm gut samples were collected from silkworm breeds, Double hybrid {(CSR 6 X CSR 26) X (CSR 2 X CSR 27)} obtained from Chawki rearing center (CRC) at Annur, Coimbatore.

6p caygaocaolon2 11-03-2020 7 0   Download

A total of 24 blast infected samples were collected from major finger millet growing areas of Tamil Nadu and also from All India Coordinated Small Millets Improvement Project Centres (ICAR) in India. Pathogenicity test with Magnaporthe grisea isolates showed highly significant differences in virulence on the variety KM 252 in the severity of leaf blast. Among the five different media tested, the Oat Meal Agar medium (OMA) was found to be best for the growth of M. grisea isolates and all the isolates produced white colony colour. The mean mycelial growth was maximum in OMA medium with 80.

16p cothumenhmong3 22-02-2020 35 2   Download

Basidiobolomycosis is a very rare form of subcutaneous mycosis often confused with other clinically mimicking conditions. Poor clinical suspicion and lack of experience due to its rarity sometimes misdirects clinicians, pathologists and microbiologists. Wrongful diagnosis may lead to inappropriate and fatal interventions. This present endeavour is aimed at a thorough study of those cases to readdress the challenges in diagnosis and management from eastern India.

8p chauchaungayxua3 07-02-2020 9 0   Download

The xanthan gum is an exopolysaccharide of the microbial origin, produced by the bacteria of the Xanthomonas spp. In the present study Xanthomonas spp were obtained from cabbage leaves and lemon sample. The isolates were coded as SC1 and SC4. From various cultural, morphological and biochemical characteristics the bacteria were identified as belonging to Xanthomonas spp. The bacteria were then tested for production of Xanthan in the fermentation medium. Measurement of viscosity and residual sugar was carried out. The effect of different carbon sources on its production was also tested.

12p nguathienthan2 19-12-2019 27 1   Download

Rhizome rot and pseudostem rot diseases of banana are caused by bacterial pathogens mainly of Pectobacterium carotovorum or Dickeya chrysanthemi individually or together and D. paradisiaca respectively. The bacterial rot diseases are reported to cause 40-70% incidence in severely infected fields and their occurrence has been reported worldwide.

8p quenchua2 18-12-2019 11 1   Download

Soil contains huge diversity of microorganism which produces different types of enzymes. Amylase is one of them that hydrolyses starch into its monomer compounds, the smallest being glucose. Hence, amylase is a very prevalent enzyme produced biologically by various kinds of microorganisms and used in industrial sectors for various purposes. Soil bacteria can be isolated and commercially grown in large numbers to produce a vast amount of amylase. In addition, amylases that are extracted require optimum conditions to show greatest activity.

13p kethamoi2 15-12-2019 28 0   Download

Efficiencies of local bacterial isolates in malathion degradation were investigated. Five bacterial isolates obtained from agricultural waste water were selected due to their ability to grow in minimal salt media, supplied with 250 ppm malathion as sole source of carbon and phosphorus. The purified bacterial isolates (MOS-1, MOS-2, MOS-3, MOS-4 and MOS-5) were characterised and identified using a combination of cellular profile (SDS-PAGE), genetic make up profile (RAPD-PCR), and morphological and biochemical characteristics.

5p kethamoi1 20-11-2019 12 0   Download

A total of 35 Rhizobium sp. were isolated from the root nodules of Trifolium alexandrinum (Egyptian clover) irrigated with treated tannery effluents and characterised on the basis of morphological, cultural and biochemical characteristics. Rhizospheric soils and plant parts were also analysed for metal concentrations by atomic absorption spectrophotometry.

8p vitamix 15-02-2019 24 1   Download