Cytoskeletal structure

The aim of this thesis was to gain an understanding of how the simulated microgravity condition affects the development of hucMSC by investigating the changes in cell morphology, proliferation and cytoskeleton structure of hucMSCs under in vitro condition.

27p beloveinhouse01 15-08-2021 19 4   Download

Calpain 1 behaviour toward cytoskeletal targets was inves-tigated using twoa-actinin isoforms from smooth and skel-etal muscles. These two isoforms which are, respectively, sensitiveandresistant tocalpaincleavage, interactwiththe protease when usinginvitro binding assays. The stability of the complexes in EGTA [Kd(–Ca2+)¼0.5 ± 0.1lM] was improved in the presence of 1 mMcalcium ions [Kd(+Ca2+)¼0.05 ± 0.01lM]. Location of the binding structures shows that the C-terminal domain ofa-actinin and each calpain subunit, 28 and 80 kDa, participates in the interaction....

9p fptmusic 12-04-2013 44 2   Download

The src homology 2 (SH2) domain-containing inositol 5-phosphatase 2 (SHIP2) catalyses the dephosphorylation of phosphatidylinositol 3,4,5-tris-phosphate [PtdIns(3,4,5)P3] to phosphatidylinositol 3,4-bisphosphate [PtdIns(3,4)P2]. We report the identification of the cytoskeletal protein Vinexin as a protein interacting with SHIP2.

15p dell39 27-03-2013 29 3   Download

Calpains are intracellular Ca 2+ -regulated cysteine pro-teases which mediate regulatory cleavage of specific substrates. They cover a broad range of physiological functions including proteolysis of molecules involved in cytoskeletal organization, the cell cycle, signal trans-duction, apoptosis, and protein renewal during growth and tissue regeneration. Originally found in mamma-lian skeletal muscle then in numerous organisms inclu-ding protists and plants, their expression is ubiquitous [1,2].

2p inspiron33 25-03-2013 41 4   Download

Cells offer different types of cytoskeletal anchorages: transitory structures such as focal contacts and perennial ones such as the sarcomeric cytoskele-ton of muscle cells. The turnover of these structures is controlled with dif-ferent timing by a family of cysteine proteases activated by calcium, the calpains.

12p inspiron33 25-03-2013 28 5   Download

Recent studies have established essential roles of protein kinase Ce in signaling pathways controlling various functions of microfilaments and intermediate filaments by modulating multiple cytoskeletal proteins. This review summarizes recent progress in our understanding of the roles of protein kinase Ce in the functions and signaling of microfilaments and intermediate filaments,

10p galaxyss3 07-03-2013 34 6   Download