Cytotoxicity assay

The synthesized compounds' enzyme inhibition potential was tested against a-glucosidase, AChE, and BChE enzymes. As illustrated from the results of IC50, these compounds are observed as the active inhibitory potential for these enzymes. The cytotoxic activity by hemolytic Assay revealed that these compounds have mild exposure to cytotoxicity on RBCs.

10p visergey 02-04-2024 0 0   Download

Ebook "Q’eqchi’ maya reproductive ethnomedicine" have an extensive pharmacopoeia of medicinal plants used traditionally for reproductive health and fertility, utilizing more than 60 plant species for these health treatments. Ten species were selected for investigation of their estrogenic activity using a reporter gene assay. Nine of the species were estrogenic, four of the species were also antiestrogenic, and two of the extracts were cytotoxic to the MCF-7 breast cancer cell line.

134p manmanthanhla0201 26-02-2024 2 1   Download

Liver cancer and breast cancer have high death rates and tend to increase rapidly in recent years in Vietnam and over the world. Currently, the study of bioactive compounds isolated from plants for killing cancer cells is of interest. The study aims to identify the cytotoxicity using MTT assay of Combretum quadrangulare Kurz fractions against MCF-7 cells.

10p vibego 02-02-2024 1 0   Download

As a continuation of our work on the bioactive constituents from Caesalpinia species, eight compounds (1−8) were isolated from the air-dried leaves of C. bonduc, collected from Vinh Phuc province of Vietnam. The cytotoxic activity of eight compounds (1–8) was screened on HepG2 human cancerous cells using the sulforhodamine B colorimetric assay.

8p viberkshire 09-08-2023 5 4   Download

Knema (Myristicaceae family) is a large genus, comprising over 100 species found in of South East Asian countries, among that over 10 species was found in Vietnam. Knema pachycarpa is a native tree distributed in central provinces of Viet Nam. Until now, only few chemical studies of Knema species in Viet Nam have been reported.

8p viannee 02-08-2023 6 3   Download

This study aims to study PASD1 protein as a potential target for CRC immunotherapy. A total of 90 CRC and polyps tissue samples were investigated for PASD1 RNA and protein expression using a real-time polymerase chain reaction and immunohistochemical staining, respectively. Matched patients’ peripheral blood mononuclear cells were pulsed with PASD1 peptides and measured for immunogenicity, cell cytotoxicity, and cytokine assays.

17p lyhuyenthu 31-01-2023 6 2   Download

The acute toxicity, cytotoxicity, genotoxicity and antigenotoxic effects of BC were studied. Cytotoxicity of BC was evaluated in cultured C3A hepatoma cells (HepG2/C3A) using a lactate dehydrogenase (LDH) activity assay. Acute toxicity was tested in adults Wistar rats treated with a single dose of BC.

5p vihermione 06-01-2023 13 3   Download

Marine fungi are evaluated as a potential source for new natural compounds with bioactivities of pharmaceutical values. In this study, 66 fungal strains, isolated from 37 sponge samples in Nha Trang bay, were determined for cytotoxic activity against two human cancer cell lines, including cervical cancer (Hela) and breast cancer (MCF-7) cells using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay.

7p vipagani 24-10-2022 4 2   Download

By means of the gas chromatography-mass spectrometry (GC/MS) method, 21 volatile components have been identified in the essential oils of fruits and the leaves of star anise (Illicium verum Hook. f., Illiciaceae) in Vietnam, in which, essential oil of star anise fruits has 18 components, essential oil of star anise leaves has 16 components, with content have been determined more than 98.60% total compounds of essential oil.

7p viporsche 25-10-2022 8 4   Download

In the poultry industry, quantitative analysis of chicken T cell proliferation is important in many biological applications such as drug screening, vaccine production, and cytotoxicity assessment. Several assays have been established to evaluate this immunological response in chicken cells.

12p vigandhi 23-02-2022 9 1   Download

Genomic mutations caused by cytotoxic agents used in cancer chemotherapy may cause secondary malignancies as well as contribute to the evolution of treatment-resistant tumour cells. The stable diploid genome of the chicken DT40 lymphoblast cell line, an established DNA repair model system, is well suited to accurately assay genomic mutations.

16p viaristotle 29-01-2022 7 0   Download

Silver nanoparticles (AgNPs) with their unique properties represent great promise in biological applications. However, the high toxicity of AgNPs still remains a major bottleneck and hinders their basic usage. In the present study, to improve the cytotoxicity of AgNPs, a thin layer of polydopamine (PDOP) was proposed as shell material. For this, the as-prepared citrate-stabilized AgNPs were dispersed in dopamine solution under alkaline conditions. The thickness of the PDOP layer could be manipulated simply by tuning the polymerization time.

8p dolomite36 30-12-2021 6 0   Download

Some thiazole derivatives bearing dithiocarbamic acid esters were synthesized in order to investigate their anticandidal activity and cytotoxicity. The structures of the obtained final compounds (6a–j) were confirmed by spectral data (IR, 1H NMR, 13C NMR, and MS) and elemental analysis. The anticandidal activity of the compounds was determined (6a–j) using the microbroth dilution method and their cytotoxicity was evaluated according to the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay against normal cells.

10p langthannam 29-12-2021 8 1   Download

The newly synthesized compounds were characterized and confirmed by analytical and spectroscopic data (IR, MS, 1H NMR, and 13C NMR). Pyrazolo[1,5-a]pyrimidines 5a–c and 9a–c and Schiff bases 11b–f were investigated for their cytotoxicity against four human cancer cell lines (colon HCT116, lung A549, breast MCF-7, and liver HepG2) according to SRB assay and the structure–activity relationship was discussed.

12p langthannam 29-12-2021 14 0   Download

Thiazolylpyrazoline derivatives were recently reported as potent anticancer agents. In this study, novel 1,4-phenylene-bis-N-thiocarbamoylpyrazoles (3a–h) and 1,4-phenylene-bis-pyrazolylthiazoles (5a–h) were prepared and screened for their anticancer activities against C6 (rat brain tumor cells) and HeLa (human uterus carcinoma). Anticancer activity studies were performed as a dose-dependent assay starting with eight concentrations. 5-Fluorouracil (5-FU) was used as a positive control.

11p langthannam 29-12-2021 10 0   Download

Novel 1-substituted 3-(p-isopropylphenyl)-5-phenylformazans (3a–g) were synthesized and characterized by elemental analysis, 1H NMR, and FT-IR techniques and UV-visible spectroscopy. Antiproliferative activities of 3a–g against HeLa and C6 cells were determined using the BrdU cell proliferation ELISA assay. 5-Florouracil was used as the positive control. The effects of substituents (–H, –CH3, and –I) and their positions (ortho, meta, and para) on the antiproliferative activities were evaluated.

9p tudichquannguyet 29-11-2021 8 1   Download

Tetra-coordinated nickel(II) complexes of two ONS (1, 2) and seven ONN (3a–3g) chelating 2-hydroxy3-methoxy-benzaldehyde thiosemicarbazones were synthesized. The dibasic ligands and complexes bearing PPh 3 as a coligand were characterized by means of analytical and spectroscopic data. Cytotoxic activities of the ligands and nickel(II) complexes were determined using the MTT assay in vitro against MDCK cells, and then all the compounds were tested on influenza virus replication by plaque assays. The compounds showed GI 50 values varying from concentrations of 15.9 up to 161.

14p tudichquannguyet 29-11-2021 10 1   Download

Mn(II), Co(II), Ni(II), Cu(II), and Zn(II) transition metal complexes of 2-hydroxy-5-[(E)-(4-phenyl) diazenyl] benzaldehyde oxime and 2-hydroxy-5-[(E)-(4-nitrophenyl) diazenyl] benzaldehyde oxime ligands were synthesized and characterized through NMR, IR, ESI mass, and UV analysis. DNA binding abilities of the complexes were revealed using a UV-Vis spectrophotometer with the absorption titration and competitive binding techniques. Hydrolytic and oxidative DNA cleavage activities under different conditions were also proved.

20p tudichquannguyet 29-11-2021 9 1   Download

We report herein the synthesis, characterization, and cytotoxicity evaluations of some newer oxadiazole and triazole analogues (5a–j). The cytotoxicity of all the title compounds were evaluated as per the National Cancer Institute protocol in a one-dose assay (10 µM) on nine different panels of 59 cancer cell lines. 2-{5-[(2,4-Dichlorophenoxy)methyl]- 1,3,4-oxadiazol-2-yl}phenol (5e) showed the maximum cytotoxicity among the series of ten compounds. The cytotoxicity of 5e was comparable to that of the standard anticancer drug, 5-fluorouracil, and better than that of imatinib.

18p tudichquannguyet 29-11-2021 10 2   Download

A total of 63 newly synthesized compounds were screened in terms of PARP inhibition by cellular PARylation assay in the HeLa cell line. It was found that 19 compounds significantly inhibited the H2O2 -induced cellular PARylation. The chemosensitizer effect of these compounds in cancer cells treated with doxorubicin (doxo) was investigated. It was found that the combination of potent PARP inhibitors with doxo potentiated a cytotoxic effect, similar to that of olaparib.

26p tudichquannguyet 29-11-2021 6 0   Download