Dicer structure

MicroRNAs proceeds through the different canonical and non-canonical pathways; the most frequent of the non-canonical ones is the splicing-dependent biogenesis of mirtrons. We compare the mirtrons and nonmirtrons of human and mouse to explore how their maturation appears in the precursor structure around the miRNA.

12p vibeauty 23-10-2021 7 0   Download

To enhance silencing and avoid off-target effects, siRNAs are often designed with an intentional bias to ensure that the end of the siRNA that contains the guide strand 5¢ end is less stably hybridized relative to the end containing the passenger strand 5¢ end. One means by which this is accom-plished is to introduce a terminal mismatch, typically by changing the passenger strand sequence to impair its hybridization with the guide strand 5¢ end.

10p viettel02 20-02-2013 29 3   Download