Digital droplet PCR

Primary central nervous system lymphoma (PCNSL) are rare mature B-cell lymphoproliferative diseases characterized by a high incidence of MYD88 L265P and CD79B Y196 hotspot mutations. The aim of the study was to analyze the detection rate of the MYD88 L265P and CD79B Y196 mutation in cell free DNA (cfDNA) in plasma of patients with PCNSL.

6p vishanshan 27-06-2024 1 1   Download

HPV16 and 18 are positively correlated with cervical carcinogenesis. However, HPV prevalence tends to vary according to region, nationality, and environment. The most prevalent high-risk (HR) HPV genotypes are HPV16, 52, 58, 56, 18, 33, and 45), while the low-risk (LR) genotypes are HPV6 and 11 in the Chinese population.

9p vialfrednobel 23-12-2023 3 3   Download

Endocrine therapy is frequently administered in patients with hormone dependent (HR+) metastatic endometrial cancer. The aim of this study was to explore the incidence and clinical relevance of circulating ESR1 mutations in patients treated by AI or megestrol acetate (M) for advanced endometrial carcinoma.

9p vialfrednobel 23-12-2023 4 3   Download

Part 2 book "Protocols for the diagnosis of pig viral diseases" includes content: Lateral flow assay for diagnosis of pig viral diseases; droplet digital PCR based diagnosis for porcine viral diseases; protocols for immunofluorescence techniques; polymerase spiral reaction (PSR) for the diagnosis of porcine viral diseases; recombinase polymerase amplification based diagnostics of porcine viral diseases; cell culture system for porcine virus isolation and propagation,... and other contents.

180p muasambanhan02 18-12-2023 5 4   Download

Thyroid cancer is the most frequent malignancy of the endocrine system, of which papillary thyroid cancer (PTC) is the predominant form with a rapid increasing incidence worldwide. Rearranged during transfection (RET) fusions are common genetic drivers of PTC and the potent RET inhibitor selpercatinib has been recently approved for treating advanced or metastatic RET fusion-positive thyroid cancer.

9p visharma 20-10-2023 8 2   Download

Part 1 book "Vaccine design, methods and protocols (Vol 1 - Vaccines for human diseases)" includes content: Clinical impact of vaccine development, future challenges for vaccinologists, principles of vaccination, proteomic monitoring of b-cell immunity, application of droplet digital PCR to validate rift valley fever vaccines, methods to evaluate novel hepatitis C virus vaccines, designing efficacious vesicular stomatitis virus vectored vaccines against ebola virus,.... and other contents.

491p oursky05 20-09-2023 0 0   Download

PCR kỹ thuật số vi giọt (Droplet Digital PCR - ddPCR) là phương pháp PCR sử dụng hệ thống vi giọt, cho phép đo lường hàng nghìn sự kiện khuếch đại độc lập trong một phản ứng đơn lẻ. Bài viết trình bày ứng dụng PCR kỹ thuật số vi giọt trong định lượng tuyệt đối nồng độ ARN.

7p viwolverine 07-07-2023 8 1   Download

Bài viết bước đầu định lượng đột biến JAK2 V617F bằng kỹ thuật PCR kỹ thuật số (ddPCR) để chẩn đoán và theo dõi điều trị ở những bệnh nhân tân sinh tăng sinh tủy (MPN) có đột biến JAK2 V617F (đột biến điểm tại vị trí V617).

8p visnape 11-01-2023 14 3   Download

Mutations involving BRAF and TERT are important predictors of disease severity in thyroid cancer, but molecular testing is limited by cost and lack of adequate tissue sample. This study aimed to assess the utility of BRAFV600E and TERT testing using droplet digital PCR (ddPCR) as a diagnostic and prognostic tool for thyroid fine needle aspirate biopsy (FNAB).

9p vimahuateng 26-11-2021 8 1   Download

The purpose of this study was to develop a fit-for-purpose methylation sensitive restriction enzyme (MSRE) based digital droplet PCR (ddPCR) assay to examine MLH1 promoter methylation in ctDNA in advanced CRC.

10p vimahuateng 26-11-2021 10 3   Download

Studies suggest that mutations in the CTNNB1 gene are predictive of response to immunotherapy, an emerging therapy for advanced hepatocellular carcinoma (HCC). Analysis of circulating tumor DNA (ctDNA) offers the possibility of serial non-invasive mutational profiling of tumors.

7p vianrose2711 27-04-2021 8 2   Download

PCNA-associated factor, the protein encoded by the KIAA0101/PCLAF gene, is a cell-cycle regulated oncoprotein that regulates DNA synthesis, maintenance of DNA methylation, and DNA-damage bypass, through the interaction with the human sliding clamp PCNA.

12p vianrose2711 27-04-2021 9 1   Download

Novel biomarkers and molecular monitoring tools hold potential to improve outcome for patients following resection of pancreatic ductal adenocarcinoma (PDAC). We hypothesized that the combined longitudinal analysis of mutated cell-free plasma KRAS (cfKRASmut ) and CA 19–9 during adjuvant treatment and follow-up might more accurately predict disease course than hitherto available parameters.

10p vianrose2711 27-04-2021 7 1   Download

It is still unclear whether epidermal growth factor receptor (EGFR) mutation of primary lung adenocar‑ cinoma can be detected on sputum samples. This study aimed to examine EGFR mutations of primary lung adeno‑ carcinoma in sputum samples using droplet digital polymerase chain reaction (ddPCR) and compare it with an EGFR mutation in surgically resected lung cancer.

11p vimichigan2711 26-03-2021 13 2   Download

Bài viết trình bày việc xây dựng phương pháp PCR kỹ thuật số kết hợp với tạo vi giọt (digital droplet PCR, ddPCR) nhằm phân tích các đột biến liên quan đến điều trị đích UT phổi trên gen EGFR từ các mẫu sinh thiết lỏng (ctDNA). Phương pháp ddPCR được xây dựng có ngưỡng phát hiện đột biến ở tần suất 0.5-1% (nghĩa là 5-10 phân tử DNA ung thư trong 10.000 phân tử DNA bình thường).

7p viankanra2711 15-09-2020 49 1   Download

During posttreatment surveillance of head and neck cancer patients, imaging is insufficiently accurate for the early detection of relapsing disease. Free circulating tumor DNA (ctDNA) may serve as a novel biomarker for monitoring tumor burden during posttreatment surveillance of these patients.

8p vidhaka2711 31-07-2020 15 2   Download

Novel non-invasive biomarkers for gastric cancer (GC) are needed, because the present diagnostic methods for GC are either invasive or insensitive and non-specific in clinic. The presence of stable circulating microRNAs (miRNAs) in plasma suggested a promising role as GC biomarkers.

10p vibaku2711 22-07-2020 11 1   Download

Circulating tumour DNA (ctDNA) may serve as a measure of tumour burden and a useful tool for non-invasive monitoring of cancer. However, ctDNA is not always detectable in patients at time of diagnosis of metastatic disease.

8p vimanila2711 29-06-2020 12 1   Download

On-target resistance mechanisms found in one-third of patients receiving anaplastic lymphoma kinase (ALK) tyrosine kinase inhibitors (TKIs) are secondary ALK mutations in ALK-rearranged non-small cell lung cancer (NSCLC).

8p vimanila2711 25-06-2020 7 1   Download

MYCN amplification directly correlates with the clinical course of neuroblastoma and poor patient survival, and serves as the most critical negative prognostic marker.

10p viputrajaya2711 22-06-2020 11 1   Download