Direct fluorescent antibody test

Rabies is a highly fatal viral infection of central nervous system affecting all warm blooded animals including humans. The virus belongs to the Rhabdoviridae family, genus Lyssa virus. Several techniques have been standardized internationally for the diagnosis of rabies. This study was designed to assess the efficacy of Seller's staining test (SST) and Direct Fluorescent Antibody Test (dFAT) for diagnosis of rabies antigen, Mouse Innoculation Test (MIT) and real time reverse transcriptase polymerase chain reaction (qRT-PCR) were used as the Gold Standards.

7p trinhthamhodang1215 23-09-2020 8 1   Download

Detection of viral antigen by direct fluorescent antibody test (FAT) from clinical samples collected from cattle and buffaloes of Navsari, Surat and Valsad Districts of Southern Gujarat were carried out. Out of a total of 116 clinical samples (44-cattle, 72-buffaloes) tested, fluorescence was observed in 14 (12.09%) samples. Species-wise, eight cattle (11.11%) and 6 buffaloes (13.64%) were found to be positive by the test. The highest number of animals were detected from Surat (14.29%) followed by Valsad (12.07%) and least in Navsari (10.81%) district. Viral antigen detected in 8.

6p gaocaolon5 14-06-2020 9 1   Download