Disk diffusion method

Ebook "Modelling electroanalytical experiments by the integral equation method" begins with models independent of spatial coordinates, goes on to address models in one dimensional space geometry and ends with models dependent on two spatial coordinates. Bieniasz considers both semi-infinite and finite spatial domains as well as ways to deal with diffusion, convection, homogeneous reactions, adsorbed reactants and ohmic drops.

409p nhanphanguyet 28-01-2024 4 2   Download

This study aimed to characterize the methicillin-resistant S. aureus strains circulating on the skin of individuals with acne. S. aureus isolates were screened for methicillin resistance by methods including, oxacillin agar (OA), cefoxitin disk diffusion (CDD), and PCR for the mecA gene.

9p viplato 02-01-2024 4 2   Download

The objective of the experiment "Efficacy of a commercial supplement added to drinking water in broilers fed aflatoxin-contaminated diets" was to evaluate effects of water supplementation with a commercial supplement (VitalSear) on growth performance, mortality and serum concentrations of aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) in broilers fed diets with aflatoxin (AF) contamination.

9p tieuvulinhhoa 22-09-2022 8 2   Download

Osteomyelitis is the infection and inflammation of the bone. Inappropriate use of antibiotics and multidrug resistance has raised the morbidity and mortality rate in chronic osteomyelitis. This study aims to determine the bacterial profile and antimicrobial susceptibility patterns of chronic osteomyelitis with special mention to various resistant mechanisms. Samples from osteomyelitis cases were aerobically cultured and isolates from culture positives were identified by standard procedures. Antimicrobial susceptibility testing was done by Kirby Bauer disk diffusion method.

9p chauchaungayxua12 29-07-2021 13 2   Download

In this study, five extracts and fractions of the aerial parts of Ludwigia hyssopifolia (G. Don) Exellincluding n-hexane, chloroform, ethyl acetate, methanol, and ethanol ones were screened for anti-H. pylori activity by disk diffusion method.

6p nguaconbaynhay11 07-04-2021 17 1   Download

The present study was carried out to detect the sensitivity pattern and prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) at tertiary care hospital, Surendranagar. Total 1776 Clinical specimens were studied. All the isolates were identified by standard microbiological procedures like gram staining, colony morphology on solid culture media and biochemical reactions. Antibiotic sensitivity testing was done for all isolates by modified Kirby Bauer disk diffusion method. Total 194 Staphylococcus aureus were tested for MRSA by Cefoxitin disc diffusion test...

8p cothumenhmong9 18-01-2021 19 1   Download

This study focused on establishing the presence of Campylobacter at the farm and retail level and evaluated the antibiogram profile of isolated species. Campylobacter spp. were isolated on mCCDA agar and confirmed by API Campy (biomereaux, France) and the susceptibility profile determined by Kirby-Bauer disk diffusion method.

8p nguathienthan8 20-10-2020 9 1   Download

Carbapenems are one of the β-Lactam antibiotics with a broad spectrum of antibacterial activity. Inappropriate use of this antibiotic can produce resistance strains by production of carbapenemases enzyme. The Klebsiella pneumoniae carbapenemase (KPC) is the most important mechanism of enzymatic resistance seen in Enterobacteriaceae isolated especially Klebsiella pneumoniae. The aim and objectives of this study was isolation and detection of the Carbapenem resistant Klebsiella species strains with phenotypic methods. Out of 11248 samples, 602 Klebsiella species (33.

6p nguaconbaynhay8 13-10-2020 11 1   Download

Escherichia coli is the most common organism causing urinary tract infection (UTI). This organism has the ability to produce Extended Spectrum Beta Lactamases (ESBLs), which confer multiple drug resistance making urinary tract infection difficult to treat. So treatment of UTI requires constant updating of the antibiotic sensitivity profile. Objectives of this study were to detect prevalence of ESBL production among E. coli isolates causing urinary tract infection and to detect their antibiotic susceptibility pattern.

8p trinhthamhodang1215 23-09-2020 25 1   Download

Coagulase-negative staphylococci (CNS) are a main cause of nosocomial infection. The main purpose of this study was to determination of frequency of CNS isolates in in hospitalized patients and their susceptibility pattern to antimicrobial agents. During 11 months study, 147CNS clinical isolates were recovered from hospitalized patients in NMC & hospital Nellore south India. The age of patients was 10 t0 70yrs. In vitro susceptibility of isolates to 14 antimicrobial agents – ampicilin, oxacillin, cefexime. azithromycin, oflaxacin, clindamycin, amikacim, tegycyclin, vancomycin, tegycyclin.

8p cothumenhmong7 08-09-2020 20 1   Download

Antimicrobial resistance is a growing threat worldwide. Increasing resistance to third generation cephalosporins has become a cause for concern. The prevalence of extended spectrum–lactamases (ESBLs) and metallobetalactamases (MBL) among members of Enterobacteriaceae constitutes a serious threat to current -lactam therapy leading to treatment failure. A total of 276 Gram negative isolates were processed. ESBL was detected by phenotypic confirmatory disc diffusion test (PCDDT) using ceftazidime alone and in combination with clavulanic acid.

8p angicungduoc6 20-07-2020 19 1   Download

This study aimed to determine bacteriological quality of indoor air in neonatal wards at tertiary hospitals and assess antibiotic resistance patterns of isolated bacteria. Passive air sampling was performed to appraise bacteriological indoor air quality employing the gravity settle plate method to monitor air quality in the morning and afternoon. Each procedure was performed in duplicate on Nutrient Agar plates for 30 and 60 minutes. A total of 75 bacteria were isolated and subjected to antibiotic sensitivity tests using the Kirby-Bauer’s disk diffusion method.

9p nguaconbaynhay6 23-06-2020 8 0   Download

Staphylococcus epidermidis (S. epidermidis) is a common pathogen in ocular infection. Mutations contribute to drug resistance. We intended to identify mutations in genes within the quinolone resistance determining region (QRDR) of fluoroquinolone-resistant S. epidermidis ocular isolates and to study their phenotypic and genotypic correlation. A total of 50 phenotypically fluoroquinolones-resistant S. epidermidis isolates were studied. Fluoroquinolones susceptibility was evaluated by Kirby- Bauer disk diffusion method.

11p trinhthamhodang1213 30-05-2020 10 1   Download

This situation prompts early and accurate detection of MBL producers. Hence the present study was done to phenotypically evaluate the prevalence of MBL production among 235 clinical isolates of Pseudomonas aeruginosa (183) and Acinetobacter species (52). The antimicrobial susceptibility testing was done by Kirby Bauer disk diffusion method.

11p trinhthamhodang5 16-05-2020 13 1   Download

Coagulase-negative Staphylococci (CoNS) have emerged as predominant pathogens in hospital and community acquired infections. Clindamycin is a good therapeutic agent in treatment of skin, soft tissue as well as serious infections caused by both methicillin sensitive and resistant Staphylococci. However, strains with inducible clindamycin resistance often lead to therapeutic failure. Hence, each laboratory should be aware of the local prevalence of this resistance among clinical staphylococcal isolates.

10p cothumenhmong4 25-03-2020 10 0   Download

Acinetobacter calcoaceticus baumannii (ACB) is a significant pathogen in hospitals with a growing incidence of multi-drug resistant (MDR) strains. This was a prospective study carried out on ACB isolates from clinical samples between 2013 and 2015. Anti-microbial susceptibility testing was done by Kirby-Bauer Disk Diffusion method (KBBD) method. CRAB strains were tested by Modified Hodge test (MHT) to detect MBL producers. Of the 190 ACB isolates, 142 (75%) were MDR strains. 93% were hospital acquired and 7% community acquired. MDR was seen only in hospital acquired isolates.

11p cothumenhmong4 25-03-2020 16 1   Download

In the present study 128 samples collected from 66 ducks of various breeds like Batak(28), Moti(34) and Khaki Campbell (6) out of which 112 number of samples from 52 dead birds and 16 number of samples from live birds. blood, liver, swabs from femur bone marrow and also swabs from foot lesions were collected consists of 128 samples in which blood sample (12), swabs from foot lesions(26) of live birds and liver(22), lungs(30) and swab from bone marrow (38) of dead birds. All the samples were processed for routine microbial isolation and identification.

9p cothumenhmong3 22-02-2020 18 2   Download

In the present study 86 number of skin scrapings were collected from various breeds like Labrador retriever (n=32), German shepherd (n=21), Spitz (n=9), Dacshund (n=16), Mastiff (n=5), Boxer (n=3) and age groups from 6 months to, above 3 year with characteristics ring like lesions, alopecia, irregular patches of hair loss. All the dogs were presented to teaching veterinary clinical complex, Odisha veterinary college from December 2018 to June 2019.On routine identification of fungus out of 86 number of skin scrappings it was revealed that arthospores were present in 54 number of samples.

6p cothumenhmong3 22-02-2020 16 1   Download

Neonatal sepsis is one of the significant reasons for morbidity and mortality among the neonates in the developing countries. To determine the bacterial isolates and their antibiotic susceptibility pattern. Blood cultures were performed for all clinically suspected neonatal septicemia cases for 1 year. Identification of all pathogenic isolates was done by conventional methods followed by antibiotic sensitivity testing by Kirby bauer disk diffusion method. A total of 225 suspected for neonatal septicemia were included in this study. Among this 78(34.

6p nguathienthan3 27-02-2020 26 0   Download

Infection of sterile body fluid can lead to severe morbidity and mortality. Periodic surveillance and monitoring programmers are helpful for framing the antibiotic policy. The present study was undertaken to evaluate aerobic bacteriological profile along with their antibiogram from various sterile fluids over a period of five years. A total of 1708 body fluid samples were processed according to Standard guidelines and analysed in order to assess the changing trends in bacteriological profile and antibiogram.

10p cothumenhmong3 22-02-2020 30 2   Download