Divergent primer

Since circular RNAs (circRNAs) post-transcriptionally regulate gene expression, they have attracted increasing attention. However, there is no existing tool to annotate and extract spliced sequences for circRNAs and no tool to determine the specificity of circRNA primers.

5p viconnecticut2711 28-10-2020 13 1   Download

Random Amplified Polymorphic DNA (RAPD) Markers were use to determined the genetic variability of eight divers cowpea (Vigna unguiculata L. walp) genotypes from different eco-geographical regions of India. Total primers 40 out of there 35 could be generated DNA fragment were 1479 but were as 1159 showed polymorphic pattern with 78.34 per cent. The polymorphism was scored and used in band sharing analysis to identify genetic relationship between the genotypes.

8p caygaocaolon3 09-03-2020 29 1   Download

Twenty four genotypes of Arachis hypogaea (L.), of which 12 genotypes belonging to Virginia and 12 belonging to Spanish varieties were used to study the genetic divergence within its botanical varieties using RAPD 16 primers belonging to OPH were used in the study.

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Random amplified polymorphic DNA- Polymerase chain reaction (RAPDPCR) was employed to assess the genetic variability and phylogenetic relation among fifteen breed of Gangatiri animals Viz. fourteen animals (cow no.288, 348, 394, 365, 313, 364, 293, 319, 283, 208, 306, 322, 476, 478) are both sexes and one bull were selected randomly. The DNA samples were isolated from a total of fifteen animals from Gangatiri cows and three random primers were selected. The genetic distance was found higher between cow no. 288 and 476 (D = 0.82697) and lower (D = 0.17647) between cow no 478 and 476.

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The present investigation was to study the molecular diversity of twelve cotton genotypes, in order to select the suitable divergent parent for heterosis breeding. Totally, 55 primers were used to diversify these genotypes and 40 pairs showed clear, scorable and unambiguous bands. Out of 40, 25 primer pairs (62.55 %) were found to be polymorphic and 15 found to be monomorphic. The average number of alleles per locus was 1.8. The similarity coefficient ranged from 0.56 to 1.00 with high dissimilarity coefficient of 0.44 for the genotypes TCH 1777 with MCU 13 and TSH 0499 with SVPR 4.

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Information on allelic differentiation is very important to elucidate the nature and extent of divergence among Brassica juncea genotypes. In present study 20 Brassica juncea L. genotypes generated 79 polymorphic alleles from 15 SSR primer pairs with an average of 5.26 alleles per locus. The polymorphism percentage was recorded to be the maximum in the case of the primer Ni4- D04 (80%) and the minimum in the case of 3 different primers namely A06-20686249, Ni3-D03 and A05-20242013 (33.3%). The polymorphism information content (PIC) values ranged from 0.41 (A01-13393871) to 0.

11p chauchaungayxua3 07-02-2020 12 0   Download

Genetic diversity using Random Amplified Polymorphic DNA (RAPD) markers was assessed in forty eight Indian potato germplasm. Twenty RAPD markers amplified a total of ninety-one different loci that exhibited 82.30 per cent polymorphism among 48 potato germplasm. The PIC value ranged from 0.27 to 0.55. All the loci amplified by the primer which were found to be polymorphic varied in size from 1500bp. The Jaccard’s similarity coefficient was found to vary from 0.22 to 0.87. The maximum genetic similarity (0.87) was found between Kufri Badshah and Kufri Khyati and lowest (0.

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Alu transposons are found only in primate genomes and have accumulated in large numbers since primates diverged from other mammals. Human chromosomes contain more than one million Alu copies, equaling about 10% of the genome by mass. This accumulation was made possible by a transposition mechanism that reverse transcribes Alu mRNAs into mobile DNA copies. Another transposon, the long interspersed element (LINE) L1, supplies a specialized reverse transcriptase enzyme needed for Alu to jump. Hence, Alu and L1 exist in a sort of molecular symbiosis.

35p hoangphiyeah1tv 24-04-2013 42 4   Download