DNA amplification by Polymerase Chain Reaction

In this study, we investigated the protection of EGCG for DNA single strand breaks in irradiated cells using primers for amplification of RNA polymerase, an enzyme responsible to synthesis of mRNA from the damaged DNA, through its down-regulated capacity.

4p vidumbledore 06-01-2023 10 2   Download

Keratin-associated proteins are fibrillar proteins and are the vital components of hair and wool fibres as they are part of matrix of fibres and form a cross- linked network with the keratin intermediate filaments. Variation in sequence and expression pattern of KAP may affect fibre characteristics. In this study, we used direct DNA sequencing to analyse the goat HGT-KAP 7.1gene which codes for the protein responsible for the fineness of fibre.

5p caygaocaolon8 07-11-2020 10 1   Download

Salmonellosis is one of the most common causal food borne disease in India. An in-house loop-mediated isothermal amplification (LAMP) reaction was established and evaluated for sensitivity and specificity in detecting the presence of Salmonella spp. isolates in foods of animal origin from Mumbai, India. The aim of this study was to develop in-house LAMP for simple and inexpensive detection of Salmonella spp. in animal origin foods using specifically designed primers targeting invA gene contains sequences unique to this genus. The reaction was optimized using genomic DNA of S.

10p angicungduoc6 20-07-2020 23 1   Download

Insecticidal δ-endotoxins of Bacillus thuringiensis have acquired great significance in recent years because of their specificity to target insects and toxicity at very low concentrations. Genes from B. thuringiensis have been the most successful group of genes identified for use in genetic transformation of crops for pest control on a commercial scale. Successes of plant transformation include most of major economic and vegetable crops. Polymerase chain reaction (PCR) is commonly used technique for the presence of transgene in the putative transformants by DNA amplification.

17p kethamoi4 16-04-2020 17 1   Download

Molecular variation in chilli fruit rot pathogen (Alternata alternata) was analyzed by using six isolates collected from major chilli growing region of Maharashtra. The genomic DNA extracted from each isolate of A. alternata was subjected to polymerase chain reaction using 15 primers of OPA and OPB series off which 10 primers produced 83 scorable bands with size ranging from Among the RAPD primer 83 bands 80 bands were polymorphic and level of polymorphism was 96.38%. Molecular diversity using RAPD marker showed that the Aa2 (Satara) having higher similarity index with Aa4 (Akola).

7p trinhthamhodang1212 06-04-2020 10 1   Download

DNA fingerprinting, estimation of molecular genetic diversity, marker assisted breeding and molecular diagnostic tools are the need of the hour for sugarcane crop improvement. Efficient extraction of genomic DNA (gDNA) free from polysaccharides, polyphenols, RNA and other major contaminants is a key to all these molecular activities. We present herewith, a modified DNA extraction protocol that has been optimized for isolating gDNA from endergonic and hardy sugarcane plant and its phytopathogens and endophytic fungi.

9p chauchaungayxua3 07-02-2020 9 0   Download

The aim of this study was to determine the phylogenetic relationships of Trichoderma isolates obtained from NLS Tobacco region. Utilizing the sequence analysis of internal transcribed spacer-1 (ITS-1) region of the ribosomal DNA the rDNA of Five Trichoderma isolates was amplified by polymerase chain reaction (PCR) using universal primers (ITS-1 and ITS-4). PCR products were purified and these purified products were used to amplify the ITS-4 region of the five Trichoderma isolates.

8p chauchaungayxua3 07-02-2020 33 0   Download

Cassava (Manihot esculenta Crantz) is one of the most important crops in Vietnam for providing food, starch sources, and raw materials for the production of bio-ethanol and other purposes. Cassava bacterial blight (CBB) disease, caused by Xanthonomas axonopodis pv. manihotis (Xam), is one of the most important factors affecting cassava production in Vietnam. A rapid and sensitive molecular tool is required to support the traditional method, primarily based on biochemical reactions, that was considered less sensitive and time-consuming.

6p caygaocaolon1 13-11-2019 25 1   Download

The greatest change since the last edition has been increasing use of nucleic acid probes (“DNA Probes”), especially with amplification by the polymerase chain reaction, in bacterial and viral infectious disease, leukemia and lymphoma phemotyping, and genetic diagnosis. Another significant trend has been proliferation of immunologic tests for prognosis of varying types of cancer, especially breast carcinoma.

0p gian_anh 18-10-2012 43 2   Download