DNA Barcoding data
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The study using molecular data from three DNA regions (matK, rbcL, and trnH-psbA) supported the monophyly of Melodinus. The M. cochinchinensis from Vietnam and China are closely related. There are some genetic variations in the sequences of Melodinus cochinchinensis from Belgium versus Asiatic M. cochinchinensis, as well as the phylogenetic position of the species, suggesting the possibility of misidentification of M. cochinchinensis from Belgium.
7p giangnhanly 02-10-2023 8 3 Download
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A new species Hyperaxis phuquocnia sp. nov. is described and illustrated from Phu Quoc National Park, Kien Giang province, southern Vietnam based on female specimens. Color photos of habitats and genitalia, and DNA Barcoding data of the new species are presented. An updated key to all species of the genus Hyperaxis in Vietnam is provided.
9p vicaptainmarvel 21-04-2023 11 2 Download
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The DNA metabarcoding approach has become one of the most used techniques to study the taxa composition of various sample types. To deal with the high amount of data generated by the high-throughput sequencing process, a bioinformatics workflow is required and the QIIME2 platform has emerged as one of the most reliable and commonly used.
14p vihagrid 30-01-2023 8 3 Download
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Genotyping of the human leucocyte antigen (HLA) is indispensable for various medical treatments. However, unambiguous genotyping is technically challenging due to high polymorphism of the corresponding genomic region. Next-generation sequencing is changing the landscape of genotyping. In addition to high throughput of data, its additional advantage is that DNA templates are derived from single molecules, which is a strong merit for the phasing problem.
12p vilarryellison 29-10-2021 11 0 Download
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DNA barcoding is a useful tool in identifying species, biodiversity assessment, and revealing phylogenetic relationships of living organisms in the world. However, the DNA barcode data for leaf beetles in Vietnam is lacking. In this study, sixteen DNA sequences of 658 bp of COI gene from nine species (five genera; three subfamilies) of Chrysomelidae in Vietnam were (obtained). Intra- and inter-specific diversities, and phylogenetic relationships of these species were analyzed.
7p nguaconbaynhay10 20-02-2021 13 3 Download
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Adapter trimming is a prerequisite step for analyzing next-generation sequencing (NGS) data when the reads are longer than the target DNA/RNA fragments. Although typically used in small RNA sequencing, adapter trimming is also used widely in other applications, such as genome DNA sequencing and transcriptome RNA/cDNA sequencing, where fragments shorter than a read are sometimes obtained because of the limitations of NGS protocols.
12p vikentucky2711 26-11-2020 10 0 Download
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DNA barcodes are short unique sequences used to label DNA or RNA-derived samples in multiplexed deep sequencing experiments. During the demultiplexing step, barcodes must be detected and their position identified. In some cases (e.g., with PacBio SMRT), the position of the barcode and DNA context is not well defined.
16p vikentucky2711 26-11-2020 12 1 Download
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Objective of this study was to identify selected species of Cordyceps genus using DNA barcodes. Seven strains of Cordyceps were collected. Total DNA extraction and purification, PCR amplification and DNA sequencing were performed with standard chemicals and kits. The candidate ITS1-5.8S-ITS2 region was amplified and sequenced. Data were analyzed using Bioedit 7.2.6 and MEGA 7 softwares. Analysis of seven obtained DNA barcode sequences of collected samples revealed that the ITS1- 5.8S-ITS2 region provided high species discriminating power for Cordyceps genus.
8p nguyenxuankha_bevandan 14-08-2020 17 1 Download
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Among the members of Halymeniaceae family, Grateloupia sensu lato occupies the largest composition in species. Classification based on morphological traits is difficult due to the highly variable terete to blade-like thalli among the members of this genus that usually leads to misidentification. Molecular systematics has been applied to classify Grateloupia sensu lato so that the taxonomists acquire a better understanding of the species diversity in general.
13p trinhthamhodang1214 06-08-2020 8 1 Download
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This paper study conducted a selection of five specific DNA sequences to use as DNA barcodes which are: matK, rbcL, trnH-psbA, ITS2 and ycflb.
7p 12120609 18-03-2020 10 1 Download
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Lichen is a combination of two organisms one is alga and another one is fungus. There is symbiotic relationship between alga and fungus. The algal part provides nutrition to the whole organism and the fungus part provides structural support to the organism.
11p cothumenhmong3 22-02-2020 13 0 Download
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Huyen-sam (Vietnamese name) which belongs to Scrophularia L. genus is a valuable herb. This medicinal plant is classified as Scrophularia ningpoensis Hemsl. Huyen-sam roots, which contain a large amount of bioactive compounds, have a similar morphology to its relatives. DNA barcodes promise to be a precise and reliable tool for distinguishing the processed Huyen-sam materials from their counterfeits. However, studies about using DNA barcodes for classification of Scrophularia L. in Vietnam are not available. Here, we conducted a taxonomic analysis of eight Scrophularia L.
9p caygaocaolon1 13-11-2019 9 0 Download