DNA isolation

The Insecta are the most diverse group of animals on the planet. The diversity of Plecoptera aquatic insect order is used as an environmental and ecological indicator. The study of endemic species is important for confirming the national biodiversity and contributing to the determination of national sovereignty. Despite Vietnam being a biodiversity hotspot, the number of known insect species remains limited.

11p viambani 18-06-2024 3 1   Download

This study aims to (1) evaluate the genetic diversity of antibacterial yeast isolated from natural honey and beeswax in Son La province by RAPD (Random Amplified Polymorphic DNA) and (2) study some biological characteristics of them.

12p viamancio 04-06-2024 2 0   Download

The present study aims to identify Neofusicoccum isolates collected from lotus with symptoms of dieback, blight, and wilted leaves. Species were identified based on their morphology and comparison of the DNA sequence data for ITS rDNA and β-tubulin (tub-2). The taxonomy and phylogenetic relationships of pathogen isolates were investigated, and their pathogenic abilities were determined.

17p viritesh 02-04-2024 5 1   Download

In this study, a total of 30 yeast isolates were recovered from local fruits in Thua Thien Hue province. Genetic characterization based on the ITS sequences identified isolates belonging to 3 species including Saccharomyces cerevisiae, Lachancea fermentati, and Clavispora fructus, with high sequence homology (over 99%) compared to published sequences in the GenBank.

11p viritesh 02-04-2024 3 1   Download

Part 2 book "Biomedical tissue culture" includes content: Placental structure and biological aspects of fetal membranes cultured in vitro; tissue development and mechanical property in the regenerated cartilage tissue; the art of animal cell culture for virus isolation; tissue based model of HCV replication as a replacement for animal models in drug testing; tissue culture to assess bacterial enteropathogenicity; viral DNA and CDNA array in the diagnosis of respiratory tract infections.

124p muasambanhan07 20-02-2024 2 0   Download

DNA isolation is one of the important steps in conducting PCR-based or real-time PCR-based molecular analysis. This study aims to test the quality of DNA isolation extracted from samples of processed food products from chicken meat (chicken nuggets).

7p vibego 02-02-2024 0 0   Download

Ebook "Isolation and molecular characterization of circulating tumor cells" discusses approaches for enrichment and isolation of CTCs as well as recent advances in comprehensive molecular profiling of CTCs using cutting-edge omics technology. This book offers comprehensive discussions on the current understanding of the molecular biology of CTCs, as well as highly advanced and in-depth approaches for molecular characterization of these cells.

304p lucchinguyen 28-12-2023 4 2   Download

Part 1 book "Protocols for the diagnosis of pig viral diseases" includes content: Requirements and preparedness for attending a viral disease outbreak in pig farms; collection of samples, their preservation and transportation; methods for quantification of viruses; protocols for isolation of genetic materials from RNA viruses; multiplex pcr for diagnosis of porcine diseases; protocols for isolation of plasmid DNA,... and other contents.

203p muasambanhan02 18-12-2023 6 3   Download

Part 1 book "Environmental microbiology - Methods and protocols" includes content: Methods for isolation and cultivation of filamentous fungi, rapid extraction of PCR-competent DNA from recalcitrant environmental samples, quantitative PCR for detection of mRNA and gDNA in environmental isolates, analysis of community dynamics in environmental samples using denaturing gradient gel electrophoresis, terminal restriction fragment length polymorphism (T-RFLP) profiling of bacterial 16S rRNA genes,... and other contents.

134p oursky10 04-12-2023 4 2   Download

Part 1 book "PCR Protocols" includes content: Long range PCR with a DNA polymerase fusion, isolation of genomic insertion sites of proviruses using splinkerette PCR based procedures, lariat dependent nested PCR for flanking sequence determination, a global single-Cell cDNA amplification method for quantitative microarray analysis,...and other contents.

171p oursky06 17-10-2023 5 1   Download

The results showed that 02 bacterial strains and 06 actinomycete strains were selected from 32 isolates. Then, we optimized the growing conditions of the screened microorganisms. Among actinomycetes strains PU1.1, PU2.1 gave the highest weight loss results, therefore they were selected for further study. Our results showed that TG2.1 and CM4.1 are Bacillus subtilis. PU1.1 is Streptomyces lilacs, while PU1.2 is Streptomyces Misiones. The selected microorganisms belong to risk group level 1 (safety group).

10p nhanchienthien 25-07-2023 4 2   Download

A molecular docking study of three natural acridones, namely citrusinine-I, 5-hydroxynoracronycin, and paratrimerin C on a target enzyme DNA polymerase μ (Pol μ), was performed. In addition, the hydrogen peroxide anion (HOO─ ), a model radical, and delavirdine, a synthetic drug, are used as ligands for docking on this target protein to investigate the activity of the selected compounds to protect Pol μ protein against their harmful effects.

7p nhanchienthien 25-07-2023 3 2   Download

The sperm gene bindin encodes a gamete recognition protein, which plays an important role in conspecific fertilization and reproductive isolation of sea urchins. Molecular evolution of the gene has been extensively investigated with the attention focused on the protein coding regions.

16p vinarcissa 21-03-2023 2 1   Download

In this paper we investigate de novo deletions defined as DNA segments missing in an oral cleft proband but present in both unaffected parents. We compare de novo deletion frequencies in children of European ancestry with an isolated, non-syndromic oral cleft to frequencies in children of European ancestry from randomly sampled trios.

9p vinarcissa 21-03-2023 1 1   Download

For this purpose, primary hBM-MSCs with wild type (WT, C/C), heterozygote (HTZ, C/T) and homozygote (HMZ, T/T) for the MTHFR gene were obtained with ethical committee permission and donor informed. Mutations were detected using RFLP and Sanger sequencing methods from genomic DNA isolated from cells, colonization properties were investigated by CFU-F test and proliferative differences were investigated by MTT test.

13p lyhuyenthu 31-01-2023 7 2   Download

Alterations in the epidermal growth factor receptor were confirmed to take a significant role in targeted therapy for patients with lung cancer. Studied 299 non-small cell lung cancer patients to determine the distribution of EGFR mutation and itsrelationship with clinicopathological characteristics of patients.

5p visirius 11-01-2023 4 2   Download

The MCNP 6.2 event-by-event transport of electrons has been used for the first time to simulate energy imparted in low-density objects corresponding to nanometric unit-density objects. It is shown that the isolated atom assumption and the use of an average excitation energy based on stopping power, significantly alters the value of energy imparted from excitations.

12p vironald 15-12-2022 11 3   Download

(BQ) Ebook Plant Reverse Genetics: Methods and Protocols – Part 2 presents the following content: Chapter 10: methods for rice phenomics studies; chapter 11 : development of an efficient inverse pcr method for isolating gene tags from t-dna insertional mutants in rice; chapter 12: transposon insertional mutagenesis in rice; chapter 13: reverse genetics in medicago truncatula using tnt1 insertion mutants; chapter 14: screening arabidopsis genotypes for drought stress resistance; chapter 15 : protein tagging for chromatin immunoprecipitation from arabidopsis.

151p runordie7 30-08-2022 5 2   Download

Cordyceps sp. CPA14V was isolated from insect-fungi sample that was collected from Copia - Son La Nature Reserve. The strain was able to biosynthesize cyclooligomer depsipeptides (CODs). This study aimed to identify Cordyceps sp. CPA14V to species using DNA sequence analysis and to estimate the effects of carbon, nitrogen, pH of culture broth on its growth and COD producing capacity.

7p vimelindagates 18-07-2022 10 2   Download

N-acetyltransferase 13 (NAT13) is a probable catalytic component of the ARD1A-NARG1 complex possessing alpha (N-terminal) acetyltransferase activity. Results: In this study, a full-length complementary DNA (cDNA) encoding Schistosoma japonicum NAT13 (SjNAT13) was isolated from schistosome cDNAs. The 621bp open reading frame of SjNAT13 encodes a polypeptide of 206 amino acids.

11p vidarwin 22-02-2022 16 1   Download