DNA synthesis methods

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Facile synthesis and grafting of N2O2-Cu(II) Schiff base complex onto graphene oxide surface: Aspects of its antimicrobial, anticancer, antiCOVID-19 and photodegradation of methylene blue

In this study, a facile synthesis and grafting method is reported for tetradentate Cu(II) Schiff base complex onto the surface of graphene oxide (GO). The Schiff base Cu-complex was synthesized through the condensation reaction between 2,3-diamino pyridine and 2-hydroxy-1-naphthaldehyde, followed by the addition of Cu(II) ion to afford N2O2-Cu(II) complex.

17p dianmotminh02 03-05-2024 3 1 Download

Ebook Genome instability: Methods and protocols

Ebook "Genome instability: Methods and protocols" presents forty-two methods and protocols to analyze diverse aspects of genome instability. Chapters detail mutagenesis and repair, methods to quantify and analyze the properties of DNA double-strand breaks, profile replication, replication proteins strand-specifically, genome instability, fluorescence microscopic techniques, and genomic and proteomic approaches.

654p lucchinguyen 28-12-2023 6 2 Download

Synthesis of quaternized zinc(II) and cobalt(II) phthalocyanines bearing pyridine-2-yl-ethynyl groups and their DNA binding properties

The synthesis and biological properties of tetra-substituted zinc and cobalt phthalocyanines bearing ethynylpyridine units on the periphery and their quaternized derivatives have been reported. The binding of quaternized ZnPc and CoPc (Q-ZnPc and Q-CoPc) with calf thymus DNA was investigated by UV-Vis spectrophotometric methods and thermodynamics. The mixtures of Q-ZnPc or Q-CoPc and DNA solutions were used to determine the change in the thermal melting temperature of DNA with the thermal denaturation profile.

14p tudichquannguyet 29-11-2021 11 1 Download
Synthesis of 2-substituted 8-propargyloxyquinoline derivatives and determination of their antioxidant, antibacterial, and DNA binding activities

2-Substituted 8-propargyloxyquinoline derivatives have been synthesized from 2-substituted-8-hydroxyquinolines by O-propargylation method (30%–98% yields). The newly synthesized compounds were tested for in vitro antioxidant activities such as DPPH radical scavenging, ferrous chelating, and reducing power activities. The maximum radical scavenging (46.5%) and reducing power activities were obtained from 1 and maximum ferrous chelating effect was obtained from 6 (72.1%) at the concentration of 500 µg/mL. To indicate DNA binding activity of the complexes calf thymus DNA was used.

12p tudichquannguyet 29-11-2021 14 1 Download
Grid-based computational methods for the design of constraint-based parsimonious chemical reaction networks to simulate metabolite production: GridProd

Constraint-based metabolic flux analysis of knockout strategies is an efficient method to simulate the production of useful metabolites in microbes. Owing to the recent development of technologies for artificial DNA synthesis, it may become important in the near future to mathematically design minimum metabolic networks to simulate metabolite production.

9p viconnecticut2711 28-10-2020 12 1 Download
A highly parallel strategy for storage of digital information in living cells

Encoding arbitrary digital information in DNA has attracted attention as a potential avenue for large scale and long term data storage. However, in order to enable DNA data storage technologies there needs to be improvements in data storage fidelity (tolerance to mutation), the facility of writing and reading the data (biases and systematic error arising from synthesis and sequencing), and overall scalability.

19p vihamax2711 21-04-2020 10 0 Download
DNA-Templated Organic Synthesis: Natures Strategy for Controlling Chemical ReactivityApplied to Synthetic Molecules**

EPA Region 9 has developed technical guidelines to help ensure that sampling and analyzing for Volatile Organic Compounds (VOCs) in soil and solid matrices are conducted in a manner that achieves accurate, technically defensible data. Region 9’s guidelines, which are intended to apply whenever VOC sampling in and analysis of soil and solid matrices are conducted, are consistent with United States Environmental Protection Agency (USEPA) Office of Solid Waste test methods.

23p cao_can 02-01-2013 66 3 Download
DNA Replication Methods and Protocols

DNA replication is fundamental to cellular life on earth, and replication initiation provides the primary point of control over this process. Replication initiation in all organisms involves the interaction of initiator proteins with one or more origins of replication in the DNA, with subsequent regulated assembly of two replisome complexes at each origin, melting of the DNA, and primed initiation of DNA synthesis on leading and lagging strands.

691p bachduong1311 07-12-2012 53 4 Download
Biopolymers

Biopolymers are polymers produced by living organisms. Cellulose, starch, chitin, proteins, peptides, DNA and RNA are all examples of biopolymers. This book comprehensively reviews and compiles information on biopolymers in 30 chapters. The book covers occurrence, synthesis, isolation and production, properties and applications, modification, and the relevant analysis methods to reveal the structures and properties of some biopolymers.

0p cucdai_1 22-10-2012 102 17 Download