Double haploid inducer
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Artificial synthesis of octoploid rapeseed double haploid (DH) induction lines Y3380 and Y3560 was made possible by interspecific hybridization and genome doubling techniques. Production of pure lines by DH induction provides a new way to achieve homozygosity earlier in B.napus.
13p vijichea2711 28-05-2021 19 1 Download
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Microspore embryogenesis describes a stress-induced reprogramming of immature male plant gametophytes to develop into embryo-like structures, which can be regenerated into doubled haploid plants after whole genome reduplication. This mechanism is of high interest for both research as well as plant breeding.
16p vihashirama2711 21-05-2020 14 1 Download
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In vivo haploid induction (HI) based on Stock6-derived inducer lines has been the most prevalent means of producing haploids. Nevertheless, the biological mechanism of HI is not fully understood, the twinembryo kernels had been found during haploid induction, which may provide potential evidence for the abnormal double fertilization during HI.
9p vishikamaru2711 29-04-2020 12 1 Download
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Liquid Murashige and Skoog (MS) medium in varying strengths, is the most commonly utilized medium for proficient rooting in the regenerated plants in doubled haploidy breeding protocols. On the other side, CPV (cocopeat: perlite: vermiculite) medium is currently being used in hardening of tissue culture regenerated plants.
9p angicungduoc4 26-04-2020 15 1 Download
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Doubled haploid induction is one of the available methods normally used for sugar beet breeding. Gynogenic haploid explants of sugar beet induced with 1 or 2 mg L–1 6-benzylaminopurine were treated with 5 g L–1 colchicine, then subcultured on a solidified MS medium plus 0.2 mg L–1 kinetin.
9p caplock2711 21-02-2019 10 1 Download