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Dried blood spots
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Single-strand conformational polymorphism (SSCP) is still a frequently used genotyping method across different fields for the detection of single nucleotide polymorphisms (SNPs) due to its simplicity, requirement for basic equipment accessible in most laboratories and low cost.
12p
vinarcissa
21-03-2023
3
1
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The thesis is structured as follows: Chapter 1 reviews the literature and outlines the importance of blood, clinical use of blood, current methods of blood collection including alternative methods and the benefits of DBS. Chapter 2 summaries the overall methods used in the project. Chapter 3 explains the utility of hemaPEN® from both the collection and analytical perspective. Chapter 4 expands on the HCT bias associated with standard DBS method. Chapter 5 expands on the use of DBS in other fields such as genomic and epigenomics.
164p
runthenight04
02-02-2023
4
1
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As there is a lack of research evaluating the implementation of DBS sampling for GBT2Q in community settings, we aimed to evaluate this intervention, identifying key facilitators and ongoing challenges to implementing community-based DBS screening for HIV/HCV among GBT2Q.
10p
viferrari
28-11-2022
7
2
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In large epidemiological studies it is often challenging to obtain biological samples. Self-sampling by study participants using dried blood spots (DBS) technique has been suggested to overcome this challenge. DBS is a type of biosampling where blood samples are obtained by a finger-prick lancet, blotted and dried on filter paper.
9p
vialabama2711
21-09-2020
17
0
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Dried blood spots (DBS) have been proposed as potentially tool for detecting invasive bacterial diseases. DBS detected pneumococcal and H. influenzae DNA in children with pneumonia and healthy.
8p
vimanama2711
28-07-2020
7
1
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Five diseases are currently screened on dried blood spots in France through the national newborn screening programme. Tandem mass spectrometry (MS/MS) is a technology that is increasingly used to screen newborns for an increasing number of hereditary metabolic diseases.
10p
virubber2711
21-03-2020
11
0
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The aim of this study was to develop an economical ‘in-house’ single round polymerase chain reaction (PCR) assay using filter paper-dried blood spots (FP-DBS) for early infant HIV-1 diagnosis and to evaluate its performance in an African setting.
8p
virubber2711
21-03-2020
11
0
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Neonatal bloodspot screening (NBS) identifies conditions to offer early intervention and minimize irreversible damage. NBS policies guide a comprehensive system including processes for storage of neonatal dried blood spots (NDBS). NDBS retention and secondary use policies have been subject of public debates internationally, suggesting that the public’s perceptions of NDBS policy are not always on par with existing policies.
11p
virome2711
13-01-2020
10
0
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Infants infected during pregnancy or while breastfeeding requires early HIV diagnosis at 6 weeks after birth to identify HIV infection and timely treatment. The objective of this work was to determine the prevalence and associated risk factors of HIV among HIV exposed infants in the Tigray regional state, Northern Ethiopia.
8p
virome2711
13-01-2020
8
0
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Patients with beta-thalassemia major (BTM) suffer from fatigue, poor physical fitness, muscle weakness, lethargy, and cardiac complications which are related to an energy crisis. Carnitine and acylcarnitine derivatives play important roles in fatty acid oxidation, and deregulation of carnitine and acylcarnitine metabolism may lead to an energy crisis. The present study aimed to investigate carnitine and acylcarnitine metabolites to gain an insight into the pathophysiology of BTM.
12p
trinhthamhodang1
14-11-2019
19
2
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