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Droplet digital polymerase chain reaction
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Esophageal cancer (EC) is a deadly disease with limited therapeutic options. Although circulating tumor DNA (ctDNA) could be a promising tool in this regard, the availiable evidence is limited. We performed a systematic review and meta-analysis to summarize the clinical applicability of the next-generation sequencing (NGS) and droplet digital polymerase chain reaction (ddPCR) technology on the ctDNA detection of the EC and listed the current challenges.
12p
vikoch
27-06-2024
1
1
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Mutations involving BRAF and TERT are important predictors of disease severity in thyroid cancer, but molecular testing is limited by cost and lack of adequate tissue sample. This study aimed to assess the utility of BRAFV600E and TERT testing using droplet digital PCR (ddPCR) as a diagnostic and prognostic tool for thyroid fine needle aspirate biopsy (FNAB).
9p
vimahuateng
26-11-2021
8
1
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It is still unclear whether epidermal growth factor receptor (EGFR) mutation of primary lung adenocar‑ cinoma can be detected on sputum samples. This study aimed to examine EGFR mutations of primary lung adeno‑ carcinoma in sputum samples using droplet digital polymerase chain reaction (ddPCR) and compare it with an EGFR mutation in surgically resected lung cancer.
11p
vimichigan2711
26-03-2021
12
2
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MYCN amplification directly correlates with the clinical course of neuroblastoma and poor patient survival, and serves as the most critical negative prognostic marker.
10p
viputrajaya2711
22-06-2020
10
1
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