Dynamic binding

Bài giảng môn Lập trình mạng: Chương 5 - DHTML & Client sript có nội dung trình bày các tính chất của DHTML, các sự kiện có thể kết hợp hàm xử lý, Dynamic Style, Dynamic font, Data Binding, thí dụ về viết script.

75p talata_1 22-09-2014 78 9   Download

In this lecture we learned about: Overriding a method, static and dynamic binding, virtual function, types of member function, abstract method/abstract class, constructor and destructor: virtual or non-virtual.

29p nanhankhuoctai10 23-07-2020 17 2   Download

The thesis is structured as follows: In Chapter 1, the scientific literature is summarized within the scope of this study. Chapter 2 provides a comprehensive overview of the principles of the applied computational approaches in this study. Chapter 3 identifies the important residues of the LsIA/α7 nAChR complex that affect the interactions between the toxin and the membrane protein due to C-terminal carboxylation of LsIA. The effects of C-terminal modification of LsIA on interactions with α3β2 nAChR, involved in cardiovascular diseases, were also investigated in Chapter 4.

234p runthenight04 02-02-2023 13 3   Download

Bài giảng Lập trình hướng đối tượng: Virtual method và Polymorphism trình bày các nội dung chính như: Nối kết tĩnh (Static binding), nối kết động (Dynamic binding), chạy chương trình, bài toán thực tế, phương thức ảo,...và một số nội dung khác

12p namthangtinhlang_02 05-11-2015 51 3   Download

Porcine heart cytoplasmic malate dehydrogenase (s-MDH) is a dimeric protein (2 · 35 kDa). We have studied equilibrium unfolding and refolding of s-MDH using activity assay, fluorescence, far-UV and near-UV circular dichroism (CD) spectroscopy, hydrophobic probe-1-anilino8-napthalene sulfonic acid binding, dynamic light scattering, and chromatographic (HPLC) techniques. The unfolding and refolding transitions are reversible and show the presence of two equilibrium intermediate states.

11p system191 01-06-2013 41 4   Download

There is much evidence to indicate that cholesterol forms lateral membranemicrodomains (rafts), and to suggest their important role in cellular signaling. However, no probe has been produced to analyze cholesterol behavior, especially cholesterol movement in rafts, in real time. To obtain a potent tool for analyzing cholesterol dynamics in rafts, we prepared and characterized several truncated fragments of h-toxin (perfringolysin O), a cholesterol-binding cytolysin, whose chemicallymodified formhas been recently shown to bind selectively to rafts. ...

9p tumor12 22-04-2013 41 5   Download

Escherichia coliproteinY (pY) binds to the small ribosomal subunit and stabilizes ribosomes against dissociation when bacteria experience environmental stress. pY inhibits trans-lationin vitro,most probably by interferingwith the binding of the aminoacyl-tRNA to the ribosomal A site. Such a translational arrest may mediate overall adaptation of cells to environmental conditions. We have determined the 3D solution structure of a 112-residue pY and have studied its backbone dynamic by NMRspectroscopy. ...

10p tumor12 22-04-2013 35 2   Download

cAMP receptor protein (CRP), allosterically activated by cAMP, regulates the expression of several genes inEscheri-chia coli. As binding of cAMP leads to undefined conform-ational changes in CRP, we performed a steady-state and time-resolved fluorescence study to showhow the binding of the ligand influences the structure and dynamics of the protein. We used CRP mutants containing a single trypto-phan residue at position 85 or 13, and fluorescently labeled with 1,5-I-AEDANS attached toCys178.

11p tumor12 20-04-2013 33 3   Download

Retinol-binding protein transports retinol, and circulates in the plasma as a macromolecular complex with the protein transthyretin. Under acidic con-ditions retinol-binding protein undergoes a transition to the molten globule state, and releases the bound retinol ligand. A biased molecular dynamics simulation method has been used to generate models for the ensemble of conformers populated within this molten globule state.

13p fptmusic 12-04-2013 42 3   Download

Transmembrane proton translocation in the photosynthetic membranes of the purple bacteriumRhodobacter sphaero-ides is driven by light and performed by two transmem-brane complexes; the photosynthetic reaction center and the ubiquinol–cytochromecoxidoreductase complex, coupled by two mobile electron carriers; the cytochrome and the quinone. This paper focuses on the kinetics and thermo-dynamics of the interaction between the lipophylic electron carrier ubiquinone-10 and the photosynthetic enzyme reconstituted in liposomes. ...

11p fptmusic 12-04-2013 61 2   Download

Human cells contain two homologs of the yeast RAD23 protein, hHR23A and hHR23B, which participate in the DNA repair process. hHR23B hou-ses a domain (residues 277–332, called XPCB) that binds specifically and directly to the xeroderma pigmentosum group C protein (XPC) to initiate nucleotide excision repair (NER). This domain shares sequence homology with a heat shock chaperonin-binding motif that is also found in the stress-inducible yeast phosphoprotein STI1.

10p awards 06-04-2013 35 3   Download

The solution structure ofNereis diversicolorsarcoplasmic calcium-binding protein (NSCP) in the calcium-bound form was determined by NMR spec-troscopy, distance geometry and simulated annealing. Based on 1859 NOE restraints and 262 angular restraints, 17 structures were generated with a rmsd of 0.87 A ˚ from the mean structure. The solution structure, which is highly similar to the structure obtained by X-ray crystallography, includes two open EF-hand domains, which are in close contact through their hydrophobic surfaces....

15p awards 06-04-2013 55 3   Download

A hallmark ofa-crystallin-type small heat shock proteins (sHsps) is their highly dynamic oligomeric structure which promotes intermolecular interactions involved in subunit exchange and substrate binding (chaperone-like activity). We studied the oligomeric features of two classes of bacterial sHsps by size exclusion chromatography and nanoelectro-spray mass spectrometry.Proteins of both classes formed large complexes that rapidly dissociated upon dilution and at physiologically relevant heat shock temperatures....

10p dell39 03-04-2013 45 3   Download

The catalytic competence of the natural thrombin mutant with deletion of the Lys9 residue in the A-chain (DK9) was found to be severely impaired, most likely due to modification of the 60-loop conformation and catalytic triad geometry, as supported by long molecular dynamics (MD) simula-tions in explicit water solvent.

11p dell39 27-03-2013 39 4   Download

The physiological phenomenon that the antisweet taste effect of gymnemic acid (GA) is diminished by application of c-cyclodextrin (c-CD) to the mouth was evaluated at the molecular level using isothermal titration calorimetry, NMR and dynamic light scattering. These analyses showed that GA specifically binds to c-CD.

7p dell39 27-03-2013 50 4   Download

Sanguinarine has been shown to inhibit proliferation of several types of human cancer cell including multidrug-resistant cells, whereas it has min-imal cytotoxicity against normal cells such as neutrophils and keratino-cytes. By analyzing the antiproliferative activity of sanguinarine in relation to its effects on mitosis and microtubule assembly, we found that it inhibits cancer cell proliferation by a novel mechanism.

12p inspiron33 26-03-2013 42 4   Download

Previous cross-linking studies [Kim E, Bobkova E, Hegyi G, Muhlrad A & Reisler E (2002)Biochemistry41, 86–93] have shown that site-specific cross-linking among F-actin monomers inhibits the motion and force generation of actomyosin. However, it does not change the steady-state ATPase parameters of actomyosin.

10p inspiron33 26-03-2013 38 5   Download

Inorganic phosphate (Pi) and cofilin⁄actin depolymerizing factor proteins have opposite effects on actin filament structure and dynamics. Pi stabilizes the subdomain 2 in F-actin and decreases the critical concentration for actin polymerization. Conversely, cofilin enhances disorder in subdomain 2, increases the critical concentration, and accelerates actin treadmilling.

9p inspiron33 26-03-2013 58 5   Download

Chemokines elicit their function by binding receptors of the G-protein-cou-pled receptor class, and the N-terminal domain (N-domain) of the receptor is one of the two critical ligand-binding sites. In this study, the thermo-dynamic basis for binding of the chemokine interleukin-8 (IL-8) to the N-domain of its receptor CXCR1 was characterized using isothermal titra-tion calorimetry.

11p inspiron33 23-03-2013 45 4   Download

Many studies have investigated the intracellular trafficking of Shiga toxin, but very little is known about the underlying dynamics of its cellular recep-tor, the glycosphingolipid globotriaosyl ceramide. In this study, we show that globotriaosyl ceramide is required not only for Shiga toxin binding to cells, but also for its intracellular trafficking.

14p inspiron33 23-03-2013 33 3   Download