Dynamic light scattering

The main aims of this research are: To determine the phase inversion temperatures for the formation of oil-in-brine nanoemulsions at various salt concentrations using conductivity measurements; To prepare the nano-emulsions at PIT points, and study their stability by measuring their droplet size, polydispersity and size distribution using dynamic light scattering technique; To confirm the DLS results using Cryo-SEM technique;...

324p runthenight07 01-03-2023 8 4   Download

This study focuses on the stability of nano-emulsions produced by phase inversion temperature method using n-Decane and n-Dodecane as the dispersed phase and pure water/brine as the aqueous phase (continuous phase). A non-ionic surfactant (Brij 30) was used in the preparation of the nano-emulsions. Three different types salt namely \(NaCl\), \(KCl\) and \(MgC{l_2}\) were used at different concentrations.

173p runthenight07 01-03-2023 7 3   Download

Porcine heart cytoplasmic malate dehydrogenase (s-MDH) is a dimeric protein (2 · 35 kDa). We have studied equilibrium unfolding and refolding of s-MDH using activity assay, fluorescence, far-UV and near-UV circular dichroism (CD) spectroscopy, hydrophobic probe-1-anilino8-napthalene sulfonic acid binding, dynamic light scattering, and chromatographic (HPLC) techniques. The unfolding and refolding transitions are reversible and show the presence of two equilibrium intermediate states.

11p system191 01-06-2013 39 4   Download

Many organelle enzymes coded for by nuclear genes have N-terminal sequences, which directs them into the organelle (precursor) and are removed upon import (mature). The experiments described below charac-terize the differences between the precursor and mature forms of water-melon glyoxysomal malate dehydrogenase. Using recombinant protein methods, the precursor (p-gMDH) and mature (gMDH) forms were puri-fied to homogeneity using Ni 2+ –NTA affinity chromatography.

0p awards 05-04-2013 48 1   Download

The physiological phenomenon that the antisweet taste effect of gymnemic acid (GA) is diminished by application of c-cyclodextrin (c-CD) to the mouth was evaluated at the molecular level using isothermal titration calorimetry, NMR and dynamic light scattering. These analyses showed that GA specifically binds to c-CD.

7p dell39 27-03-2013 48 4   Download

Changes in the oligomeric status of MutS protein was probed in solution by dynamic light scattering (DLS), and corroborated by sedimentation ana-lyses. In the absence of any nucleotide cofactor, free MutS protein [hydro-dynamic radius (Rh) of 10–12 nm] shows a small increment in size (Rh 14 nm) following the addition of homoduplex DNA (121 bp), whereas the same increases to about 18–20 nm with heteroduplex DNA containing a mismatch.

16p dell39 27-03-2013 52 4   Download

The poorly known mechanism of inhibition of cholinesterases by inorganic mercury (HgCl2) has been studied with a view to using these enzymes as biomarkers or as biological components of biosensors to survey polluted areas. The inhibition of a variety of cholinesterases by HgCl2 was investi-gated by kinetic studies, X-ray crystallography, and dynamic light scatter-ing.

13p galaxyss3 21-03-2013 50 4   Download

The formation and structure of proinsulin C-peptide oligomers has been investigated by PAGE, NMR spectroscopy and dynamic light scattering. The results obtained show that C-peptide forms oligomers of different sizes, and that their formation and size distribution is altered by salt and divalent metal ions, which indicates that the aggregation process is medi-ated by electrostatic interactions.

10p viettel02 19-02-2013 40 3   Download