Electrochemical immunosensor

Parathyroid hormone (PTH) is a polypeptide containing 84 amino acids secreted by cells of the parathyroid glands. Imbalances of parathyroid levels cause medical problems such as osteoporosis, mental disorders, pancreatitis, kidney stones, cancer, and other symptoms. In this study, we aimed to design an ultrasensitive electrochemical immunosensor for PTH detection. Indium tin oxide (ITO) was used as an electrode for electrochemical impedance spectroscopy (EIS) measurements.

14p tudichquannguyet 29-11-2021 12 1   Download

The aim of this research was to design an electrochemical immunosensor for determination of tumour necrosis factor receptor-associated protein-1(TRAP1) antigen, a heat shock protein linked to tumour necrosis factor. The indium-tin oxide covered polyethylene terephthalate (ITO-PET) electrode surface was cleaned and was prepared for the introduction of hydroxyl groups on its surface by using NH4 OH/H2 O2 /H2 O. As a silanization agent for covalent attachment of anti-TRAP1 on the surface of the ITO working electrode, 3-glycidoxypropyltrimethoxysilane (3-GOPS) was used.

11p tudichquannguyet 29-11-2021 9 1   Download

Alpha-1 antitrypsin (A1AT) is one of the acute phase proteins which are synthesized in the liver. A1AT inhibits the activity of many proteases, but its main task is to protect the lungs from the attack of neutrophil elastase. In an autosomal hereditary disease known as alpha-1 antitrypsin deficiency, the A1AT level in blood serum decreases, increasing the risk of developing emphysema, liver apoptosis, and liver cancer. Thus, the detection of A1AT concentration in blood serum is very important.

15p tudichquannguyet 29-11-2021 6 1   Download

In this study, a nonlabeled immunosensor based on covalent immobilization of anti-TSH antibody by using the formation of self-assembled monolayers (SAM) of 4-mercaptophenylacetic acid (4-MPA) and functionalization of carboxyl ends with 1-ethyl-3-(3-dimetilaminopropil) carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) was fabricated for detection of TSH. Immobilization steps including the concentration of 4-MPA, the concentration of anti-TSH antibody, and duration of anti-TSH antibody incubation were optimized by utilizing electrochemical impedance spectroscopy.

16p tudichquannguyet 29-11-2021 12 0   Download

Their use in fields of environmental pollution control and early warning is still in the early stages. Biosensors are classified according to their transduction principle such as optical, electrochemical and piezoelectric or based on their recognition element as immunosensors, apt sensors, genosensors, and enzymatic biosensors, when antibodies, aptamers, nucleic acids, and enzymes are, respectively, used.

20p chauchaungayxua10 19-03-2021 13 1   Download

This study aims to develop an electrochemical immunosensor for detecting ENaC using a screen-printed carbon electrode (SPCE), which was modified with reduced graphene oxide (RGO).

10p tocectocec 25-05-2020 10 0   Download

The ultimate goal of this paper is to develop derivatives of ferroceneservingas an electrochemical probe that would enhance the performance of immunosensor.An immunosensor based on polypyrrole-streptavidin layer as platform for immobilization of biotinylatedferrocene and antibodies was developed. The measurement of redox signal of ferrocene was analyzed by differential pulse voltammetry (DPV) method and underlined a variation of redox properties upon the antigen interaction.

10p cumeo3000 01-08-2018 30 0   Download