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Engineered nucleases
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Ebook "Site-directed insertion of transgenes" aims to present the state of this field from basic mechanisms of site-directed modifications to their applications in a wide range of organisms such as bacteria, yeast, plants, insects, mammals. It will discuss the problems encountered when using the random integration strategy and present the recent advances made in targeted genome modification.
393p
nhanphanguyet
28-01-2024
3
2
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"Development of smart fruit crops by genome editing" discuss (i) the new findings in gene editing technologies, (ii) a comparison between them, and (iii) their applications for genetic analysis and manipulation of fruit crops. Different editing technologies, especially the CRISPR/Cas9 system, were successfully used in fruit crops such as apple, banana, cacao, citrus, grape, kiwifruit, and pear.
12p
lyhuyenthu
31-01-2023
3
2
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The propensity for off-target activity of Streptococcus pyogenes Cas9 (SpCas9) has been considerably decreased by rationally engineered variants with increased fidelity (eSpCas9; SpCas9-HF1). However, a subset of targets still generate considerable off-target effects.
17p
vialfrednobel
29-01-2022
7
0
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High-fidelity SpCas9 variants (eSpCas9 and SpCas9-HF1) have been engineered to reduce off-target effects. We found that changes in guide RNA length induced significant reductions in the editing activities of SpCas9 variants in plant cells.
7p
vialfrednobel
29-01-2022
14
0
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We report that engineered Cas9 variants with improved specificity—eCas9-1.1 and Cas9-HF1—are often poorly active in human cells, when complexed with single guide RNAs (sgRNAs) with a mismatch at the 5’ terminus, relative to target DNA sequences. Because the nucleotide at the 5’ end of sgRNAs, expressed under the control of the commonly-used U6 promoter, is fixed to a guanine, these attenuated Cas9 variants are not useful at many target sites.
6p
vialfrednobel
29-01-2022
6
0
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The CRISPR/Cas9 system provides bacteria and archaea with molecular immunity against invading phages and conjugative plasmids. Recently, CRISPR/Cas9 has been used for targeted genome editing in diverse eukaryotic species.
11p
viaristotle
29-01-2022
9
0
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CRISPR-Cas systems have been broadly embraced as effective tools for genome engineering applications, with most studies to date utilizing the Streptococcus pyogenes Cas9. Here we characterize and manipulate the smaller, 1053 amino acid nuclease Staphylococcus aureus Cas9.
10p
viaristotle
29-01-2022
9
0
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Engineered endonucleases are a powerful tool for editing DNA. However, sequence preferences may limit their application. We engineer a structure-guided endonuclease (SGN) composed of flap endonuclease-1 (FEN-1), which recognizes the 3′ flap structure, and the cleavage domain of Fok I (Fn1), which cleaves DNA strands.
12p
viaristotle
29-01-2022
8
0
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AsCas12a and LbCas12a nucleases are reported to be promising tools for genome engineering with protospacer adjacent motif (PAM) TTTV as the optimal. However, the C-containing PAM (CTTV, TCTV, TTCV, etc.) recognition by Cas12a might induce extra off-target edits at these non-canonical PAM sites.
13p
viarchimedes
26-01-2022
8
1
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The recent advances in agricultural biotechnology and genetic engineering have brought numerous benefits to the food and agricultural sector by improving the essential characteristics of plant agronomic traits. Targeted genome editing using sequence specific nucleases (SSNs) provides a general method for inducing targeted deletions, insertions and precise sequence changes in a broad range of organisms and cell types.
15p
chauchaungayxua11
23-03-2021
12
2
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Practical approaches for multigene transformation and gene stacking are extremely important for engineering complex traits and adding new traits in transgenic crops. Trait deployment by gene stacking would greatly simplify downstream plant breeding and trait introgression into cultivars.
12p
viuchiha2711
21-04-2020
7
0
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Non-human primate (NHP) models can closely mimic human physiological functions and are therefore highly valuable in biomedical research. Genome editing is now developing rapidly due to the precision and efficiency offered by engineered site-specific endonuclease-based systems, such as transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPRassociated protein-9 nuclease (Cas9) system.
10p
vihamax2711
21-04-2020
12
0
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The CRISPR/Cas (clustered regularly interspaced short palindromic repeat and CRISPR-associated nucleases) based technologies have revolutionized genome engineering. While their use for prokaryotic genome editing is expanding, some limitations remain such as possible off-target effects and design constraints.
8p
vihamax2711
21-04-2020
12
0
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As with many plant species, current genome editing strategies in soybean are initiated by stably transforming a gene that encodes an engineered nuclease into the genome. Expression of the transgene results in a double-stranded break and repair at the targeted locus, oftentimes resulting in mutation(s) at the intended site.
13p
vihamax2711
21-04-2020
9
2
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emerging field Genome manipulation technology is one of emerging field which brings real revolution in genetic engineering and biotechnology. Genome editing technique is consistent for improving average yield to achieve the growing demands of world‟s existing food famine. Because of their advantages such as simplicity, efficiency, high specificity and amenability to multiplexing, genome editing technologies are revolutionizing the way crop breeding is done and paving the way for next generation breeding.
19p
nguathienthan2
19-12-2019
12
1
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