Enzymatic digestions
-
Part 1 book "Ruminant physiology - Digestion, metabolism, growth and reproduction" includes content: Integration of learning and metabolic signals into a theory of dietary choice and food intake, mathematical models of food intake and metabolism in ruminants, control of salivation and motility of the reticulorumen by the brain in sheep, molecular ecology and diversity in gut microbial ecosystems, microbial adherence to the plant cell wall and enzymatic hydrolysis,... and other contents.
210p muasambanhan07 20-02-2024 4 0 Download
-
Part 1 book "Emerging issues in fish larvae research" includes content: Investigating fish larvae microbe interactions in the 21st century - old questions studied with new tools; environmental cycles and biological rhythms during early development; the digestive function in developing fish larvae and fry - from molecular gene expression to enzymatic activity; variability in digestive enzyme capacity in early stages of marine fish larvae.... and other contents.
165p muasambanhan03 05-01-2024 6 2 Download
-
Residual biomass production for fuel conversion represents a unique opportunity to avoid concerns about compromising food supply by using dedicated feedstock crops. Developing tomato varieties suitable for both food consumption and fuel conversion requires the establishment of new selection methods.
13p vinarcissa 21-03-2023 6 1 Download
-
To understand the architecture of the plant cell wall, it is of importance to understand both structural characteristics of cell wall polysaccharides and interactions between these polysaccharides. Interactions between polysaccharides were studied in the residue after water and chelating agent extraction by sequential extractions with H2O and alkali.
10p vihermione 06-01-2023 7 2 Download
-
Biorefinery with deep eutectic solvent (DES) is an emerging processing technology to overcome the shortcomings of conventional biomass pretreatments. This work evaluates the biorefinery of sugarcane bagasse (SCB) with DES formulated with choline chloride as hydrogen bond acceptor and three hydrogen bond donors: lactic acid, citric acid, and acetic acid.
8p vihermione 30-12-2022 9 2 Download
-
Viscosity is an important rheological property, which may have impact on the glycemic response of starchy foods. However, the relationship between starch gels viscosity on its hydrolysis has not been elucidated. The aim of this work was to assess the effect of gels viscosity on the microstructure, and the kinetics of enzymatic hydrolysis of starch.
9p viginny 30-12-2022 8 2 Download
-
Slowly digestible starches have received interest due to their lower increase of postprandial blood glucose and insulin levels and, hence, modification of starches towards slower digestibility has commercial interest. However, chemical characteristics driving enzymatic (digestive) degradation are not fully unraveled.
8p viginny 30-12-2022 9 2 Download
-
Resistant starch type 3 (RS-3) holds great potential as a prebiotic by supporting gut microbiota following intestinal digestion. However the factors influencing the digestibility of RS-3 are largely unknown. This research aims to reveal how crystal type and molecular weight (distribution) of RS-3 influence its resistance.
10p viginny 30-12-2022 9 3 Download
-
Poultry feed consists mainly of conventional grains and protein supplements, however, using treated unconventional agro-industrial by-products as replacements of corn soybean-based diet can minimize production costs and improve productivity.
15p vigandhi 23-02-2022 14 1 Download
-
Acoustic or hydrodynamic shearing, sonication and enzymatic digestion are used to fragment DNA. However, these methods have several disadvantages, such as DNA damage, difficulties in fragmentation control, irreproducibility and under-representation of some DNA segments. The DNA fragmentation tool would be a gentle enzymatic method, offering cleavage frequency high enough to eliminate DNA fragments distribution bias and allow for easy control of partial digests.
11p vibeauty 23-10-2021 9 1 Download
-
Understanding the diversity of repair outcomes after introducing a genomic cut is essential for realizing the therapeutic potential of genomic editing technologies. Targeted PCR amplification combined with Next Generation Sequencing (NGS) or enzymatic digestion, while broadly used in the genome editing field, has critical limitations for detecting and quantifying structural variants such as large deletions (greater than approximately 100 base pairs), inversions, and translocations.
10p vibeauty 23-10-2021 13 0 Download
-
Infestation by tea green leafhoppers (Empoasca (Matsumurasca) onukii) can cause a series of biochemical changes in tea leaves. As a typical cell-rupture feeder, E. onukii secretes proteases while using its stylet to probe the tender shoots of tea plants (Camellia sinensis).
17p vilichoo2711 23-06-2021 14 1 Download
-
The liquid chromatography tandem mass spectrometry with electrospray ionization (ESI) source in multiple reactions monitoring (MRM) mode has been used to detect five allergen including milk, egg, peanut, soyabean, and walnut in milk, dairy products, and confectionery. The allergenic proteins from food matrices were extracted with an extraction buffer (50 mM of TRIS- saline, 2 M of urea, and 25 mM of DTT) and then enzymatically digested with trypsin to form peptides. The peptides were eventually detected on a LC-MS/MS Triple Quad 5500 system from AB SCIEX.
8p kethamoi9 01-12-2020 8 1 Download
-
Tandem mass spectrometry-based database searching is currently the main method for protein identification in shotgun proteomics. The explosive growth of protein and peptide databases, which is a result of genome translations, enzymatic digestions, and post-translational modifications (PTMs), is making computational efficiency in database searching a serious challenge.
11p vikentucky2711 26-11-2020 6 0 Download
-
Active protein translation can be assessed and measured using ribosome profiling sequencing strategies. Prevailing analytical approaches applied to this technology make use of sequence fragment length profiling or reading frame occupancy enrichment to differentiate between active translation and background noise, however they do not consider additional characteristics inherent to the technology which limits their overall accuracy.
6p vioklahoma2711 19-11-2020 8 0 Download
-
Spermatogonial Stem Cells (SSCs) are unipotent adult stem cells, defined as the undifferentiated stem cells located along the basement membrane of the seminiferous tubules. This present study is aimed to carry out the culture and characterization of SSCs in pre-pubertal age group of mice. Isolation of SSCs was done by two-step enzymatic digestion method and was seeded in a six-well plate. In primary culture (P0), colony forming unit was observed on day four post incubation. Mice Spermatogonial Stem Cells (mSSCs) adherence was observed on day eight post incubation.
7p nguaconbaynhay6 24-06-2020 19 0 Download
-
Recently, it has been indicated that anaerobic co-digestion of waste activated sludge with other waste streams at wastewater treatment plants is a promising strategy for enhancing methane production and materials recovery.
10p vihamax2711 21-04-2020 15 2 Download
-
The omental adipose tissue was collected from, the animals undergone ovariohysterectomy surgical procedures. The samples were digested with 0.1 per cent (W/V) collagenase type I and transferred to a beaker with magnetic stirrer at 600 rpm at 37oC for 30 minutes. The lesser time was given for enzymatic digestion as compared to other species. The viability of the cell was evaluated by trypan blue exclusion method using haematocytometer. Trypan blue had a high affinity to nuclear DNA, which traverse the membrane in a dead cell and dye it blue.
5p quenchua4 06-04-2020 14 1 Download
-
Morphology and phylogenetic analyses using the ITS and LSU regions of nrDNA were carried out on bolete collections belonging to Boletus s. str., originating from the Himalayan region of Pakistan. The phylogenetic analysis outlined the existence of previously unknown species of this genus. It is described here as Boletus himalayensis.
11p caplock2711 21-02-2019 37 2 Download
-
Human endometrial stem cells (hESCs) are mesenchymal stem cells, which are responsible for the monthly renewal of the basal layer of the human endometrium by facilitating stromal and vascular regeneration. In this study, hESCs were isolated by using three different isolation methods including nonenzymatic and enzymatic digestion with trypsin and collagenase type 1.
9p sony2711 28-01-2019 30 2 Download