Enzymatic substrate

Ebook "Synthesis and characterization of glycosides (Second edition)" is a short and comprehensive study on the best known approaches for preparing the main types of glycosides. It covers synthetic pathways of challenging glycosides known as antiviral or antineoplastic drugs, and synthetic substrates used for enzymatic detection, including those used for detection of gene markers in plant biotechnology. The author pays special attention to the structural characterization of glycosides and provides the basic tools for the structural assignment through NMR, X-Ray and mass spectra techniques.

432p tracanhphuonghoa1007 22-04-2024 3 2   Download

Ebook "Using mass spectrometry for biochemical studies on enzymatic domains from polyketide synthases" reports studies on the substrate specificity of crucial KS domains from trans-AT PKSs. Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, the substrate specificity of a range of KS domains from the bacillaene (BaeJ and BaeL) and psymberin (PsyA and PsyD) PKSs,...

189p tracanhphuonghoa1007 22-04-2024 6 3   Download

The aim of this study is to obtain the copper-binding protein hydrolysate from the white leg shrimp head (WLSH) by-product using enzymatic hydrolysis. The outcome indicated that the copper-binding capacity (CBC) of the WLSH protein hydrolysate achieved the maximum value of 19.4±0.5 mg Cu2+/g protein with hydrolysis conditions including Flavourzyme preparation, pH 7.5, 50°C, the enzyme : substrate (E:S) ratio of 80 U/g protein and 5h of hydrolysis.

6p visherylsandber 04-07-2022 6 2   Download

Glycosides of 4-nitrocatechol (1,2-dihydroxy 4-nitrobenzene) with α-D-glucopyranose and α-D-mannopyranose were synthesized by the glycosylation of phenol with peracetylated sugars in the presence of BF3·OBu2 . The glycoside of 4-nitrocatechol with β -D-galactopyranose was prepared by the glycosylation of this phenol as sodium phenoxide with tetra-O-benzoyl-α-D-galactopyranosyl bromide.

9p langthannam 29-12-2021 7 0   Download

The present study is aimed on isolation of OxO enzyme from Pseudomonas strain under high salt stress. The OxO enzyme was produced in bulk under selected salt stress. It was partially purified by ammonium sulphate precipitation method, dialysis and ion-exchange chromatography. The OxO enzyme on enzymatic analysis showed potent activity concerning oxalic acid substrate. The microtiter plate analysis confirmed the ability of OxO enzyme purified from Pseudomonas strain in the diagnosis of oxalate-related disorders and in the future could be used to prepare the diagnostic biosensors

6p mudbound 10-12-2021 6 1   Download

The recombinant polymerases of human influenza virus A/PR/8/34 (H1N1) (PR8) and avian influenza virus A/Turkey/England/1969 (H3N2) (TE) using anti-FLAG M2 affinity resin. After confirming trimeric complexes, enzymatic properties of recombinant polymerases were characterized, including model viral RNA binding, in vitro transcription assays, and cap-snatching activity upon addition of cap1-70 mer vRNA as substrate. Taken together we conclude that 293T cells are a suitable expression system for sufficient amount isolation of functional recombinant influenza viral polymerases with 95% of purity.

5p mudbound 10-12-2021 6 1   Download

Glucose oxidase was covalently immobilized to a porous electrode, which was developed by using ferrocene functionalized polyethyleneimine, multiwall carbon nanotubes, and carbon cloth for biofuel cell applications in our recent studies. The kinetics parameters (i.e. kM, Vmax) and other parameters required for the modeling study were determined experimentally. Fuel cell flow channel geometry was analyzed by a computational fluid dynamics modeling approach. Substrate flow rate and mass transfer for each proposed channel type were considered in the simulations.

17p tudichquannguyet 29-11-2021 5 1   Download

Cellulosimicrobium sp. MP1 was isolated from termites gut, showing ability to biosynthesize cellulase including endoglucanase, exoglucanase, -glucosidase and xylanase and therefore is potential candidate for enzymatic saccharification of lignocellulosic material. The aim of this study is to investigate the ability of crude enzyme produced from Cellulosimicrobium sp. MP1 to hydrolyse the abundant lignocellulosic material in Viet Nam as straw.

8p nguaconbaynhay11 01-04-2021 17 2   Download

Endoglucanase is one of the most important industrial enzymes. In the present study, three different fungi Aspergillus sp., Trichoderma sp. and Penicillium sp. were isolation for endoglucanase production from soil samples on PDA plate. All three fungus processed by submerged fermentation and solid state fermentation for its enzymatic activity. Aspergillus sp., showed highest enzymatic activity in both type of fermentation.

8p angicungduoc6 20-07-2020 16 1   Download

Twenty Five lignolytic fungi were isolated from decaying wood samples and were screened for the laccase production using guaiacol and ABTS as indicator compounds. The positive isolates were confirmed for the presence of laccase using Azure B and quantitative enzyme assay in presence of catalase using ABTS as substrate. Further laccase producing capacity of fungus was estimated in seven different media where Czapex Dox medium showed highest enzymatic activity of 17.2 U/ml.

15p angicungduoc6 20-07-2020 15 1   Download

The caprine milk proteins are considered as power house of bioactive peptides, these bioactive peptides may be released in enzymatic hydrolysis(Trypsin 10 mg of enzyme/5 g of protein) at pH 8.0 and temperature 400 C was of casein fractions was carried out at an enzyme- substrate (E: S) ratio of 1:25. Enzymatic hydrolysis of casein fractions was carried out at an enzyme- substrate (E: S) ratio of 1:25.

7p gaocaolon5 14-06-2020 5 1   Download

The plant biomass and agro-industrial wastes show great potential for their use as attractive low cost substrates in biotechnological processes. Wheat straw and corn cob as hemicellulosic substrates were acid hydrolyzed and enzymatically saccharified for high xylose production.

6p vijiraiya2711 27-05-2020 15 1   Download

A combined scouring and bleaching process was developed for absorbent cotton preparation using crude enzyme. The substrate for crude enzyme preparation includes banana pseudo stem dry powder, cotton seed hulls and cotton seed meal in the ratio of 60:30:10. The crude enzyme was extracted from the fermented substrate and the pectinase and laccase activity recorded in the enzyme extract was 25 and 7 Units per ml respectively.

7p chauchaungayxua5 05-05-2020 7 0   Download

Acetylation of the xylan backbone was a major obstacle to enzymatic decomposition. Removal of acetyl groups by acetyl xylan esterases (AXEs) is essential for completely enzymatic hydrolysis of xylan.

13p vihamax2711 21-04-2020 10 1   Download

Dicer is a 219-kDa protein that plays key roles in gene regulation, particularly as the ribonuclease III enzyme responsible for cleaving precursor miRNA substrates. Its enzymatic activity is highly regulated by protein factors, and this regulation can impact on the levels of miRNAs and modulate the behavior of a cell.

13p vihamax2711 21-04-2020 18 0   Download

Biologically treated paddy straw was hydrolysed using commercial cellulase and fermented to ethanol by yeast. Lignin degrading fungus Pleurotus sajor-caju removed 35.1 % lignin from paddy straw at 40 days incubation. Hydrolysis of biologically treated paddy straw with cellulase enzyme loaded at 5 FPU/g substrate at 500C resulted in about 119 mg/g sugar release. Fermentation of enzymatic hydrolysate by Saccharomyces cerevisiae resulted in production of 2.0 % ethanol after 72 h incubation at 30°C.

8p caygaocaolon3 09-03-2020 13 0   Download

This research investigated the antioxidant activity of proteolysate from Acetes japonicus. Firstly, chemical composition of the Acetes was analyzed. Then, the effect of Acetes : water ratio on protein recovery yield and the effect of the enzyme type, pH, temperature, enzyme to substrate (E:S) ratio and hydrolysis time on the antioxidant potential of the proteolysate were examined.

5p caplock2711 22-02-2019 20 0   Download

This study investigated the long-term adaptation of a rumen anaerobic fungus, Neocallimastix hurleyensis, grown on each of 3 different substrates as the sole energy source. The rumen anaerobic fungus was cultivated on Avicel, Xylan and glucose for up to 31 subcultures, and its survival and enzymatic activity were monitored.

6p vitamix 15-02-2019 17 1   Download

In this study, transformation of BrCHS var 2 into B. rotunda cell suspension culture, followed by chalcone synthase enzymatic assay and HPLC analysis was conducted to investigate whether the substrate specificity for BrCHS var 2 is either cinnamoyl-CoA or p-coumaroyl-CoA.

14p nutifooddau 27-01-2019 27 1   Download

We study the enzymatic hydrolysis of lactose by a commercial enzyme from a selected strain of Kluyveromyces fragilis. The variables analyzed were: temperature (25–40 ◦C), enzyme concentration (0.1–3.0 g l−1), lactose concentration (0.0278–0.208 M), and initial galactose concentration (0.0347 M). On the basis of the data analyzed, both published and in the present work, we propose a Michaelis–Menten kinetic model with inhibition by the product (galactose), which reveals that the substrate (lactose) and the product (galactose) present similar affinity for the active site of the enzyme.

10p abk1996 04-09-2018 31 0   Download