Enzyme preparations

In this study, in order to valorize black-purple rice, we used commercial enzyme preparations to hydrolyze the raw starch from this material to produce an anthocyanin–containing glucose solution.

11p viamancio 04-06-2024 1 0   Download

Ebook "Principles of food chemistry (Fourth edition)" was designed to serve as an introductory text for courses in food chemistry as part of food science programs meeting the Institute of Food Technologists standards. The original concept for the preparation of this book was to present basic information on the composition of foods and the chemical and physical characteristics they undergo during processing, storage, and handling.

614p tracanhphuonghoa1007 22-04-2024 9 2   Download

The Peuhl cheese (Waragashi, Warangachi, Wagashi, or Wara in local language) is a soft cheese with a high nutritional value, prepared by coagulation of whole milk under the action of calotropaïne, a plant enzyme from Calotropis procera. In this study, Plumeria alba latex has been used as an alternative source of enzymes for the production of Waragashi cheese.

6p viisac 23-09-2023 2 1   Download

Currently, great strides have been made in the field of creating highly effective synthetic medical preparations. Nevertheless, preparations derived from plant raw materials occupy a significant place in medical practice and account for about one third of the total number of medicines.

6p viisac 23-09-2023 2 1   Download

In this study, we evaluated whether bacteria can utilize the prebiotic present in soybean meal and determine the prebiotic index and activity score of the soybean meal by the newly isolated probiotic, Bacillus velezensis BS. The effect of soybean meal-containing medium on the growth, digestive enzyme secretion, and pathogenic resistance of the B. velezensis BS were also examined.

11p vimulcahy 18-09-2023 4 3   Download

Part 1 of ebook "Food chemistry (4th revised and extended edition)" provides readers with contents including: water; phase transition of foods containing water; amino acids, peptides, proteins; carbamoyl and thiocarbamoyl derivatives; mutagenic heterocyclic compounds ; enzymes; lipids; crystalline structure, melting points; carbohydrates; chemical reactions and derivatives; aroma compounds; aroma extract dilution analysis; vitamins; minerals; food additives;...

510p lytamnguyet 04-08-2023 4 3   Download

In this paper, we have prepared peripherally tetra-({6-[3-(diethylamino)phenoxy]hexyl}oxy substituted cobalt(II), copper(II), manganese(III) phthalocyanines (3, 4, 5) and their water-soluble derivatives (3a, 4a, 5a). Then, in vitro α-glucosidase and cholinesterases inhibitory actions of the water-soluble 3a, 4a, 5a were examined using spectrophotometric methods. 4a had the highest inhibitory effects among the tested compounds against α-glucosidase due to IC50 values. 4a and 5a had 40 fold higher inhibitory effects than the positive control.

11p lyhuyenthu 31-01-2023 7 2   Download

In this study, anticholinergic, antidiabetic, antioxidant and cytotoxic activities of Reseda lutea L. (R. lutea) were determined. Ethanol extracts of R. lutea (EERL) and water extract of R. lutea (WERL) were prepared for biochemical analysis. The antioxidant capacities of EERL and WERL were evaluated with 6 different methods. In addition, acetylcholinesterase (AChE), α-amylase and α-glycosidase enzyme inhibition by EERL were measured.

16p lyhuyenthu 31-01-2023 3 2   Download

The enzymatic synthesis of fructooligosaccharides (FOS) was carried out using a partially purified -fructofuranosidase from the commercial enzyme preparation Viscozyme L. Partial purification of -fructofuranosidase from Viscozyme L was done by batch adsorption using ion-exchange resin DEAESepharose, showing a 6-fold increase in specific activity.

5p vihermione 06-01-2023 11 2   Download

In order to develop safer processes for the food industry, we prepared a chitosan support with the naturally occurring crosslinking reagent, genipin, for enzyme. As application model, it was tested for the immobilization of -d-galactosidase from Aspergillus oryzae.

7p vihermione 06-01-2023 7 2   Download

A new hybrid bionanomaterial composed of zinc oxide nanoparticles (ZnO NPs) and chitosan was constructed after enzymatic immobilization of papain for biomedical applications. In this work, we report the preparation and characterization steps of this bionanomaterial and its biocompatibility in vitro.

10p viginny 30-12-2022 10 3   Download

Bài viết Nghiên cứu thu nhận dịch đạm thủy phân từ vỏ đầu tôm bằng enzyme alcalase trình bày việc tìm giải pháp sinh học để thu các amino acid và peptid (sản phẩm thủy phân protein) từ vỏ đầu tôm, từ đó tạo ra chế phẩm dịch đạm nhằm định hướng ứng dụng trong chăn nuôi. Chế phẩm dịch đạm (preparation of protein hydrolysate- PPH) từ vỏ đầu tôm được dùng làm thức ăn bổ sung trong chăn nuôi gia súc, gia cầm và trong nuôi trồng thủy sản.

6p vispyker 16-11-2022 17 2   Download

The study "Efficacy of hydrolytic enzyme preparation from Aspergillus Spp. on the fattening broiler chicken fed with corn soybean based diets" investigated the effect of enzyme preparations made of A. niger DB 106 (Cong and Hang, 2005), A. oryzae NM1 (Ly and Hang, 2006) and A. niger XP phytase (Xuan and Hang, 2006) supplemented to corn soy-bean based diets on growth performance and feed utilization efficiency of fattening chickens.

6p runordie5 04-07-2022 8 2   Download

The aim of this study is to obtain the copper-binding protein hydrolysate from the white leg shrimp head (WLSH) by-product using enzymatic hydrolysis. The outcome indicated that the copper-binding capacity (CBC) of the WLSH protein hydrolysate achieved the maximum value of 19.4±0.5 mg Cu2+/g protein with hydrolysis conditions including Flavourzyme preparation, pH 7.5, 50°C, the enzyme : substrate (E:S) ratio of 80 U/g protein and 5h of hydrolysis.

6p visherylsandber 04-07-2022 6 2   Download

The aim of the present study was to synthesize and characterize benzothiazine derivatives prepared by using 2-aminothiophenol and saccharine and in vitro screen their enzyme inhibition and antioxidant potential. Nine different derivatives were prepared and their structures were confirmed by spectral studies (EIMS, 1H and 13 C NMR). Enzyme inhibition potential of the synthesized compounds was evaluated by Ellman’s method, while antioxidant activity was determined by DPPH and FRAP assays.

9p langthannam 29-12-2021 12 0   Download

Crystallization processes of lipid matrices were characterized by X-ray diffractometry and differential scanning calorimetry. pDNA binding ability of SLNs and the stability of pDNA-SLN complexes with DNase I enzyme were also determined by gel electrophoresis. It was determined that all formulations became positively charged with DOTAP®R. They were able to bind DNA and were partially protective against enzyme degradation. Although additional studies are necessary, this study reveals the promising potential of this gene delivery system for gene therapy.

13p langthannam 29-12-2021 8 0   Download

Thioredoxin reductase (E.C 1.6.4.5.; TrxR) is an enzyme belonging to the flavoprotein family of pyridine nucleotide-disulfide oxidoreductases. In this study, mitochondrial TrxR enzyme was purified from rainbow trout mitochondria. Thanks to the 2 consecutive procedures (preparation of homogenate and 2’,5’-ADP Sepharose 4B affinity chromatography), the enzyme, having the specific activity of 11.9 EU mg protein-1, was purified with a yield of 2.38% and 672-fold. The purity of the enzyme was monitored and the molecular weight of its subunits was calculated as 70 kDa by SDS-PAGE.

10p langthannam 29-12-2021 9 0   Download

The present study is aimed on isolation of OxO enzyme from Pseudomonas strain under high salt stress. The OxO enzyme was produced in bulk under selected salt stress. It was partially purified by ammonium sulphate precipitation method, dialysis and ion-exchange chromatography. The OxO enzyme on enzymatic analysis showed potent activity concerning oxalic acid substrate. The microtiter plate analysis confirmed the ability of OxO enzyme purified from Pseudomonas strain in the diagnosis of oxalate-related disorders and in the future could be used to prepare the diagnostic biosensors

6p mudbound 10-12-2021 6 1   Download

New bis(isatins-thio/carbohydrazones) based on Schiff bases were prepared from terephthalaldehyde biscarbohydrazone and 5-substituted isatins in the presence of a drop of sulfuric acid under reflux in ethanol. Terephthalaldehyde bis(thio/carbohydrazone) was synthesized by the reaction of (thio)/carbohydrazide and terephthalaldehyde in the presence of a few drops of acetic acid under reflux in ethanol. The structures of these synthesized compounds were determined using IR, 1H NMR, and 13C NMR spectroscopy and elemental analysis.

20p tudichquannguyet 29-11-2021 14 1   Download

Polyethylene glycol (PEG) is a polymer that is widely used as a carrier for drug delivery systems (DDS). A library of N-PEGylated quinoline derivatives of PEG molecular weight 200 was prepared rapidly after the activation of PEGs using maleic anhydride. Quinoline with a polymer backbone is essential as new material. PEG is a water-soluble nonionic polymer approved by food and drug organizations for medicine applications. Because of its nontoxic grapheme, it is widely utilized in numerous biochemical, cosmetic, pharmaceutical, and industrialized applications.

20p tudichquannguyet 29-11-2021 12 1   Download