Enzyme terminal transferase

Bài giảng "Công nghệ di truyền - Chương 1: Các enzyme chủ yếu" cung cấp cho người học các kiến thức: Enzyme cắt giới hạn, Methyl hóa, Enzyme terminal transferase, phản ứng cắt bằng RE, DNA polymerase, DNA polymerase ở Prokaryote,... Mời các bạn cùng tham khảo nội dung chi tiết.

37p doinhugiobay_10 12-01-2016 152 13   Download

Bài giảng "Chương 1: Các Enzyme chủ yếu" cung cấp cho người học các kiến thức: Enzyme cắt giới hạn, Enzyme terminal transferase, phản ứng cắt bằng RE, DNA polymerase ở Prokaryote, DNA polymerase bền nhiệt, DNA polymerase bền nhiệt có hoạt tính sửa sai,... Mời các bạn cùng tham khảo.

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The a-amylase family (glycoside hydrolase family 13; GH 13) contains enzymes with approximately 30 specifi-cities. Six types of enzyme from the family can possess a C-terminal starch-binding domain (SBD): a-amylase, maltotetraohydrolase, maltopentaohydrolase, maltogenic a-amylase, acarviose transferase, and cyclodextrin glu-canotransferase (CGTase). Such enzymes are multidomain proteins and those that contain an SBD consist of four or five domains, the former enzymes being mainly hydrolases and the latter mainly transglycosidases. ...

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Classical swine fever virus nonstructural protein 5B (NS5B) encodes an RNA-dependent RNA polymerase, a key enzyme of the viral replication complex. To better under-stand the initiation of viral RNA synthesis and to establish anin vitroreplication system, a recombinant NS5B protein, lacking the C-terminal 24-amino acid hydrophobic domain, was expressed inEscherichia coli.

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Saccharomyces cerevisiaeGpi3p is the UDP-GlcNAc-bind-ing andpresumed catalytic subunit of the enzyme that forms GlcNAc-phosphatidylinositol in glycosylphosphatidylinosi-tol biosynthesis. It is anessential proteinwithanEX7 Emotif that is conserved in four families of retaining glycosyl-transferases. All Gpi3ps contain a cysteine residue four residues C-terminal to EX7E. To test their importance for Gpi3p functionin vivo,Glu289 and297 in theEX7 Emotif of S.

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