Epidermal growth

Luận án xác định sự thay đổi của HIP, EGFR ở mức độ mRNA và Protein tại mô ung thư vú so với u xơ tuyến vú lành; khảo sát sự thay đổi của HIP, EGFR ở mức độ mRNA và Protein trong các thể ung thư vú phân loại theo mô bệnh học.

39p hieuminhdo 03-09-2019 52 2   Download

The interaction of blood coagulation factor X and its Gla-containing fragments with negatively charged phospholipid membranes composed of 25 mol% phosphatidylserine (PtdSer) and 75 mol% phosphatidylcholine (PtdCho) was studied by surface plasmon resonance. The binding to 100 mol% PtdCho membranes was negligible. The calcium dependence in the membrane binding was evaluated for intact bovine factor X (factor X) and the fragment containing the Gla-domain and the N-terminal EGF (epidermal growth factor)-like domain, Gla–EGFN, from factor X....

6p system191 01-06-2013 32 4   Download

The solution structure of the growth factor chimera mEGF/ TGFa44250 has been determined using an extended version of the DYANA procedure for calculating structures from NMR data. The backbone fold and preferred orientation of the domains of the chimera are similar to those found in previous studies of EGF structures, and several H-bonds used as input constraints in those studies were found independently in the chimera. This shows that the modified activity of the chimera does not result from a major structural change.

9p system191 01-06-2013 45 4   Download

Calmodulin (CaM) is phosphorylated in vitro and in vivo by multiple protein-serine/threonine and protein-tyrosine kinases. Casein kinase II and myosin light-chain kinase are two of the well established protein-serine/threonine kinases implicated in this process. On the other hand, within the protein-tyrosine kinases involved in the phosphorylation of CaM are receptors with tyrosine kinase activity, such as the insulin receptor and the epidermal growth factor receptor, and nonreceptor protein-tyrosine kinases, such as several members of the Src family kinases, Janus kinase 2, and p38Syk....

13p research12 01-06-2013 46 6   Download

A series of chimeric receptors was generated between the epidermal growth factor (EGF) receptor, ErbB-1, and its homologue, ErbB-4, to investigate the roles of the extracellular domains (I–IV) in the ligand specificities. As compared with ErbB-1 and the chimeras with both domains I and III of ErbB-1, the chimeras with only one of these domains exhibited reduced binding of 125I-labeled EGF. Particularly, the contribution of domain III was appreciably larger than that of domain I of ErbB-1 in 125I-labeled EGF binding.

7p research12 01-06-2013 48 5   Download

The hepatocyte growth factor-regulated tyrosine kinase substrate Hrs is an early endosomal protein that is thought to play a regulatory role in the trafficking of growth factor/ receptor complexes through early endosomes. Stimulation of cells with epidermal growth factor (EGF) rapidly leads to phosphorylation of Hrs, raising the question whether the receptor tyrosine kinase phosphorylates Hrs directly. Here, we present evidence that a downstream kinase, rather than the active receptor kinase is responsible. ...

7p research12 23-04-2013 37 2   Download

Nitric oxide (NO • ) strongly inhibits the proliferation of human A431 tumour cells. It also inhibits tyrosine phos-phorylation of a 170-kDa band corresponding to the epidermal growth factor receptor (EGFR) and induces the phosphorylation at tyrosine residue(s) of a 58-kDa protein which we have denoted NOIPP-58 (nitric oxide-induced 58-kDa phosphoprotein). The NO • -induced phosphoryla-tion of NOIPP-58 is strictly dependent on the presence of EGF. Phosphorylation of NOIPP-58 and inhibition of the phosphorylation of the band corresponding to EGFR are both cGMP-independent processes. ...

10p fptmusic 16-04-2013 30 3   Download

In order to investigate the factors that determine the correct folding of epidermal growth factor-like (EGF) repeats within a multidomain protein, we prepared a series of six peptides that, taken together, span the sequence of twoEGF repeats of human tenascin, a large protein from the extra-cellular matrix. The peptides were selected by sliding a window of the average length of tenascin EGF repeats over the sequence of EGF repeats 13 and 14. We thus obtained six peptides, EGF-f1 to EGF-f6, that are 33 residues long, contain six cysteines each, and bear a partial overlap in the sequence. ...

0p awards 05-04-2013 32 2   Download

Signal transduction pathways are often embedded in com-plex networks, which result from interactions between pathways and feedback circuitry. In order to understand such networks, qualitative information on which inter-actions take place and quantitative data on their strength become essential.

9p awards 05-04-2013 47 5   Download

Gangliosideshavebeen found toreside inglycosphingolipid-enriched microdomains (GEM) of the plasma membrane and to be involved in the regulation of epidermal growth factor receptor (EGFr or ErbB1) activity.To gain further insight into the mechanisms involved in EGFr modulation by gangliosides, we investigated the distribution of EGFr family members in the plasma membrane of CHO-K1 cells, which were genetically modified to express different gan-glioside molecules or depleted of glycolipids.

10p dell39 03-04-2013 44 5   Download

Sponges (phylum Porifera) live in a symbiotic relationship with microorganisms, primarily bacteria. Until now, mole-cular proof for the capacity of sponges to recognize fungi in the surroundingaqueousmilieuhas not beenavailable.Here we demonstrate, for the demospongeSuberites domuncula (Porifera, Demospongiae, Hadromerida), a cell surface receptor that recognizes (1fi3)-b-D-glucans, e.g. curdlan or laminarin. This receptor, the (1fi3)-b-D-glucan-binding protein, was identified and its cDNA analysed.

14p dell39 03-04-2013 37 5   Download

Vaults are highly conserved, ubiquitous ribonucleoprotein (RNP) particles with an unidentified function. For the three protein species (TEP1, VPARP, and MVP) and a small RNA that comprises vault, expression of the unique 100-kDa major vault protein (MVP) is sufficient to form the basic vault structure.

12p dell39 27-03-2013 31 2   Download

Human airway trypsin-like protease (HAT), a serine protease found in the sputum of patients with chronic airway diseases, is an agonist of protease-activated receptor-2 (PAR-2). Previous results have shown that HAT enhances the release of amphiregulin (AR); further, it causes MUC5AC gene expression through the AR-epidermal growth factor receptor pathway in the airway epithelial cell line NCI-H292.

13p dell39 27-03-2013 34 1   Download

We investigated the survival signals of epidermal growth factor (EGF) in human gastric adenocarcinoma cell line TMK-1. Treatment of TMK-1 cells with adriamycin (ADR) caused apoptosis and apoptosis-related reactions such as the release of cytochromecfrom mitochondria and the activation of caspase 9.

13p inspiron33 25-03-2013 43 2   Download

Two H7721 human hepatocarcinoma cell lines showing moderate and high expression of a1,3-fucosyltransferase (FucT)-VII cDNA were established and designated FucTVII-M and FucTVII-H, respectively. In a1,3-FucT-VII-transfected cells, expression of insulin receptor (InR)a- and bsubunits and epidermal growth factor receptor (EGFR) on the cell surface and in cells, as well as the sialyl Lewis X (SLe x , the product of a1,3-FucT-VII) content of the EGFR were unchanged.

13p inspiron33 23-03-2013 41 4   Download

The mechanism behind the growth-promoting effect of insulin is a subject of debate. Employing RT4 bladder cancer cells, we examined the cross-talk between insulin and the epidermal growth factor system. We found that insulin induced a time- and dose-dependent (25–1000 nmolÆL )1 insulin) increase in mRNA expression of three ligands from the epidermal growth factor system.

11p inspiron33 23-03-2013 41 3   Download

O-Fucosylation is a post-translational glycosylation in which anO-fucose is covalently attached to the hydroxyl group of a specific serine or threon-ine residue. This modification occurs within the consensus sequence C2X4)5(S⁄T)C3 present on epidermal growth factor-like repeats of several proteins, including the Notch receptors and their ligands.

10p galaxyss3 21-03-2013 45 4   Download

Temptin, a component of the complex of water-borne protein pheromones that stimulate attraction and mating behavior in the marine mollusk Aply-sia, has sequence homology to the epidermal growth factor (EGF)-like domains of higher organisms that mediate protein–cell surface contact dur-ing fertilization and blood coagulation.

13p media19 04-03-2013 27 2   Download

Epidermal growth factor receptor (EGFR)-mediated signal transduction is often hyperactivated in tumour cells and therefore considered a promising target for cancer therapy. A number of computational models have been developed which describe the pathway in great detail.

13p media19 04-03-2013 40 3   Download

We report the development of a high-level bacterial expression system for the Alzheimer’s disease-associated amyloid b-peptide (Ab), together with a scaleable and inexpensive purification procedure. Ab(1–40) and Ab(1–42) coding sequences together with added ATG codons were cloned directly into a Pet vector to facilitate production of Met-Ab(1–40) and Met-Ab(1– 42), referred to as Ab(L1–40) and Ab(L1–42), respectively.

11p vinaphone15 27-02-2013 49 2   Download