Escherichia coli system
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This study aimed to determine the prevalence and the antibiotic susceptibility of E. coli in burn and chronic wound infection in the Le Huu Trac National Burns Hospital from January 2021 to May 2023. E. coli were identified and the antibiotics susceptibility testing was performed using the VITEK 2 automated system.
7p vioraclene 31-03-2024 1 1 Download
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Ebook "Systems biology and biotechnology of Escherichia coli" covered include genomics and functional genomics, resources for systems biology, network analysis, genome-scale metabolic reconstruction, modelling and simulation, dynamic modelling and simulation, systems-level analysis of evolution, plasmids and expression systems, protein synthesis, production and export, engineering the central metabolism, synthetic biology, and systems metabolic engineering of E. coli. This book provides readers with guidance on how a complex biological system can be studied using E. coli as a model organism.
470p cotieubac1004 15-03-2024 2 0 Download
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Part 2 book "Vaccine design, methods and protocols (Vol 2 - Vaccines for veterinary diseases)" includes content: Strategies for vaccine design using phage display derived peptides, laboratory scale production of recombinant HAA86 tick protein in pichia pastoris and in escherichia coli system; transient expression of viral proteins in plants using agrobacterium tumefaciens, recombinant botulinum toxoids - a practical guide for production,.... and other contents.
433p oursky05 20-09-2023 1 0 Download
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AcrAB-TolC tripartite efflux pump, which belongs to the RND superfamily, are the main multi-drug efflux system of Escherichia coli because of the broad resistance on antibiotics. With the discovering of efflux pump inhibitors, the combination between these and antibiotics is one of the most promising therapies.
11p vidoctorstrange 06-05-2023 5 2 Download
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Screening and expression of new β-glucosidase genes (bgc) have attracted much attention because of their valuable application in a wide range of industrial areas such as bioethanol production, food and animal feed processing, paper making, and biotechnological processes.
10p vicaptainmarvel 21-04-2023 2 2 Download
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Proper phosphate signaling is essential for robust growth of Escherichia coli and many other bacteria. The phosphate signal is mediated by a classic two component signal system composed of PhoR and PhoB.
11p vinarcissa 21-03-2023 2 1 Download
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Recent studies have reported pathogen-species-specific modulating effects on the innate immune system. Escherichia coli, Staphylococcus aureus, and Streptococcus agalactiae are important pathogenic bacteria responsible for different infectious diseases in several animal species.
11p vidarwin 23-02-2022 8 1 Download
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Constitutive and inducible defenses protect the respiratory tract from bacterial infection. The objective of this study was to characterize the response to an aerosolized lysate of killed bacteria, as a basis for studying the regulation and in vivo effects of these inducible innate immune responses.
16p vigandhi 23-02-2022 14 1 Download
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Like many bacteria, Vibrio cholerae deploys a harpoon-like type VI secretion system (T6SS) to compete against other microbes in environmental and host settings. The T6SS punctures adjacent cells and delivers toxic effector proteins that are harmless to bacteria carrying cognate immunity factors.
14p vielonmusk 30-01-2022 13 0 Download
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A high density lipoprotein (HDL)-linked enzyme with antioxidant and antiatherogenic properties, paraoxonase1(PON1), prevents the formation of atherosclerotic lesions in humans. In the present study, a recombinant hPON1 gene was produced using a small ubiquitin-related modifier (SUMO) fusion protein expression system. To that end, the hPON1 gene was amplified from human liver-ready cDNA, cloned into the expression vector pET SUMO, and expressed in Escherichia coli BL21 (DE3).
13p langthannam 29-12-2021 7 0 Download
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. In this study, the VEGF121 gene was cloned and expressed in a prokaryotic expression system (Escherichia coli). The recombinant VEGF121 was encapsulated with PLA microparticles and studied in vitro and ex ovo for the sustained release mechanism. The PLA-VEGF microparticles and the recombinant VEGF121 were explored for their bioactivity in human umbilical vein endothelial cells (HUVEC).
14p thiencuuchu 27-11-2021 8 1 Download
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The glyoxalase system usually comprises two enzymes, glyoxalase I (GLYI) and glyoxalase II (GLYII). This system converts cytotoxic methylglyoxal (MG) into non-toxic D-lactate in the presence of reduced glutathione (GSH) in two enzymatic steps. Recently, a novel type of glyoxalase III (GLYIII) activity has observed in Escherichia coli that can detoxify MG into D-lactate directly, in one step, without a cofactor.
13p visilicon2711 20-08-2021 11 1 Download
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The repertoire of RNA-binding proteins (RBPs) in bacteria play a crucial role in their survival, and interactions with the host machinery, but there is little information, record or characterisation in bacterial genomes. As a first step towards this, we have chosen the bacterial model system Escherichia coli, and organised all RBPs in this organism into a comprehensive database named EcRBPome.
6p visilicon2711 20-08-2021 10 1 Download
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The presence at high loads of certain microorganisms such as mesophilic aerobic germs, molds, coliforms, Escherichia coli, positive coagulase Staphylococci, and sulfito-reducing anaerobic bacteria was also observed in all products. The microbiological quality of these drinks is therefore not satisfactory. Consequently, the wines analyzed are not in conformity with the normative criteria.
8p chauchaungayxua10 18-03-2021 9 1 Download
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The aim of this study is to determine the antimicrobial resistance profile patterns to 15 antibiotics and heavy metals (Zn+2, Cu+2 and Cd+2) by Pantoeaagglomerans bacteria. The (MIC) of the heavy metals was varied from 200 µg /mL to 2200 µg/mL. The results showed that the bacteria were resistant to Zn+2, Cu+2 and Cd+2, considering the MIC values compared with the strain Escherichia coli K-12 used as control. P.
7p trinhthamhodang1218 26-02-2021 7 1 Download
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The temperature downshift results in (1) decrease in membrane fluidity (2) changes in nucleic acid structures (3) reduced ribosome function (4) inefficient protein folding. Bacteria respond to this low temperature change by producing cold-shock proteins (CSPs) that bind to single-stranded RNA and DNA thereby, maintaining cell physiology. CSPs have been widely studied in Escherichia coli and Bacillus subtilis. Here we discuss the cold adaptation by desaturase system, RNA chaperone, and transcription antitermination function of CspA homologues.
8p cothumenhmong9 18-01-2021 15 2 Download
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A means to predict the effects of gene over-expression, knockouts, and environmental stimuli in silico is useful for system biologists to develop and test hypotheses. Several studies had predicted the expression of all Escherichia coli genes from sequences and reported a correlation of 0.301 between predicted and actual expression.
12p vikentucky2711 26-11-2020 10 0 Download
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ntegratively analyzing multiple gene expression, microarray datasets in order to reconstruct gene-gene interaction networks. Integrating multiple datasets is generally believed to provide increased statistical power and to lead to a better characterization of the system under study.
19p vioklahoma2711 19-11-2020 11 1 Download
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The reconstruction of metabolic networks and the three-dimensional coverage of protein structures have reached the genome-scale in the widely studied Escherichia coli K-12 MG1655 strain. The combination of the two leads to the formation of a structural systems biology framework, which we have used to analyze differences between the reactive oxygen species (ROS) sensitivity of the proteomes of sequenced strains of E. coli.
23p vicolorado2711 22-10-2020 5 0 Download
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Escherichia coli is normal inhabitant of intestine of animals E. coli strains cause a wide variety of intestinal and extra intestinal diseases such as diarrhoea, urinary tract Infection, septicemia, and meningitis in neonates. Bacterial meningitis is often seen in neonatal calves as a sequele of septicemia caused by E. coli with multiple body system and organ involvement. Series of 32 calves of meningitis in neonatal calves, the post frequent clinical findings were lethargy, recumbancy, loss of suck reflex, stupper and comma.
3p quenchua8 29-09-2020 8 1 Download