Examination of complex

This project will investigate of the effects of applying scientific modes of analysis to the process of artistic production. Methodologies derived from the disciplines of classification and taxonomy will be used to examine the formal relationships between sculptural objects in respect to their conceptual and actual development. Evolutionary theory will be used as a model in this analysis. The project will investigate the complexity of the system and explore the effect of the intervention of scientific methods on artistic production.

296p runthenight07 01-03-2023 6 3   Download

The study examined the effect of education decentralisation on the practice of community participation in the Philippines. Decentralisation, as an institutional reform initiative in education, is a complex and contested concept resulting in a wide range of responses that addresses system-wide issues to improve access and quality of education. It had been a consistent theme in education reform in recent decades and its introduction brought about several permutations in application and meaning that have achieved mixed results.

271p runthenight04 02-02-2023 3 1   Download

The rationale for this study is that the social enterprise sector provides an important locale in which to situate research that aims to identify the impact of ICTs on development. Recognising the complex dynamics and range of actors involved in this diverse and emerging area of development practice, this study chooses to focus on the social enterprise sector. It is specifically concerned with mapping the external influences, use and impact of ICT on social enterprises in Cambodia.

228p runthenight04 02-02-2023 22 3   Download

This is a research topic with a wide, complex content. Within the scope of this thesis, the author focuses on the quality of examination and processing of legal documents by Provincial People’s council and People’s committeel in Vietnam, accordingly, concentrates on examining and processing the legal documents issued by Provincial People’s council and People’s committeel without extension of examination of the inspection activities of the Provincial People’s council and People’s committeel to the documents of the lower level within the province.

26p bibianh 25-09-2019 46 4   Download

Eukaryotic ribonuclease (RNase) H2 consists of one catalytic and two accessory subunits. Several single mutations in any one of these subunits of human RNase H2 cause Aicardi–Goutie`res syndrome. To examine whether these mutations affect the complex stability and activity of RNase H2, three mutant proteins of His-tagged Saccharomyces cerevisiaeRNase H2 (Sc-RNase H2*) were constructed. Sc-G42S*, Sc-L52R*, and Sc-K46W* contain single mutations in Sc-Rnh2Ap*, Sc-Rnh2Bp*, and Sc-Rnh2Cp*, respectively....

14p research12 29-04-2013 38 1   Download

Baculoviruses are well known for their large, circular, dou-ble-stranded DNA genomes. The type member, AcMNPV, is the best characterized andundergoes a successionof early, late and very late gene expression during its infection cycle. The viral genes involved inDNAreplicationhave previously been identified and their products are required for the acti-vation of late gene expression.

8p tumor12 22-04-2013 50 3   Download

The thermal unfoldingofmyosinsubfragment 1 (S1) cleaved by trypsin was studied by differential scanning calorimetry. In the absence of nucleotides, trypsin splits the S1 heavy chain into three fragments (25, 50, and 20 kDa). This cleavage has no appreciable influence on the thermal unfolding of S1 examined in the presence of ADP, in the ternary complexes of S1 with ADP and phosphate analogs, such as orthovanadate (Vi) or berylliumfluoride (BeFx), and in the presence of F-actin.

11p tumor12 22-04-2013 34 3   Download

ATP-sensitive K + channels are an octameric assembly of two proteins, a sulfonylurea receptor (SUR1)and an ion conducting subunit (Kir 6.0). We have examined the role of the C-terminus of SUR1 by expressing a series of truncation mutants together with Kir6.2 stably in HEK293 cells. Bio-chemical analyses using coimmunoprecipitation indicate that SUR1 deletionmutants andKir6.2 assemble and that a SUR1 deletion mutant binds glibenclamide with high affin-ity.

11p tumor12 22-04-2013 42 4   Download

The structure–activity relations of a series of synthetic phenoxazone drugs with aminoalkyl side chains of variable length and different terminal groups were investigated by examining their biological activity and DNA complexation affinity. Biological activitywas determined fromtheir ability to induce apoptosis and cell cycle perturbations (activation of cell cycle checkpoints) using the human malignant MOLT-3 cell line. The thermodynamic parameters of drug– DNA complexation were determined by differential scan-ning calorimetry....

8p tumor12 20-04-2013 37 4   Download

The cytochromeb559 content was examined in five types of isolated photosystem II D1-D2-cytochromeb559reaction center preparations containing either five or six chlorophylls per reaction center. The reaction center complexes were obtained following isolation procedures that differed in chromatographic column material, washing buffer compo-sition and detergent concentration. Two different types of cytochromeb559assays were performed. The absolute heme content ineachpreparationwas obtainedusing theoxidized-minus-reduced difference extinction coefficient of cyto-chrome b559 at 559 nm. ...

6p fptmusic 16-04-2013 34 2   Download

The peptide hormone endothelin transmits various signals throughGprotein-coupled receptors, the endothelin type A (ETAR) and B (ETBR) receptors. Caveolae are specialized lipid rafts containing polymerized caveolins. We examined the interaction of ETBR with caveolin-1, expressed in Sf9, COS-1, and HEK293 cells, and its effects on the subcellular distribution and the signal transduction of ETBR.

12p fptmusic 16-04-2013 27 2   Download

Dynamic protein–protein interactions are involved in most physiological processes and, in particular, for the formation of multiprotein signaling complexes at transmembrane receptors, adapter proteins and effector mole-cules. Because the unregulated induction of signaling complexes has sub-stantial clinical relevance, the investigation of these complexes is an active area of research.

10p fptmusic 12-04-2013 40 3   Download

The roles of His181, His184 and Tyr186 in PETN reductase have been examined by mutagenesis, spectroscopic and stopped-flow kinetics, and by determination of crystallographic structures for the Y186F PETN reductase and reduced wild-type enzyme—progesterone complex. Residues His181 and His184 are important in the binding of coenzyme, steroids, nitro-aromatic ligands and the substrate 2-cyclohexen-1-one.

12p fptmusic 12-04-2013 40 3   Download

There is still a controversy over the mechanism of promoter DNA strand separation upon open transcription complex (RPo) formation by Escheri-chia coli RNA polymerase: is it a single or a stepwise process controlled by Mg 2+ ions and temperature? To resolve this question, the kinetics of pseudo-first-order oxidation of thymine residues by KMnO4 in the )11…+2 DNA region of RPo at thekPRpromoter was examined under single-hit conditions as a function of temperature (13–37
C) in the absence or presence of 10 mmMgCl2....

16p awards 06-04-2013 44 2   Download

This minireview series examines the structural principles underlying the biological function of RNA-binding proteins. The structural work of the last decade has elucidated the structures of essentially all the major RNA-binding protein families; it has also demonstrated how RNA recognition takes place. The ribosome structures have further integrated this knowledge into principles for the assembly of complex ribonucleoproteins. Structural and biochemical work has revealed unexpectedly that several RNA-binding proteins bind to other proteins in addition to RNA or instead of RNA....

10p awards 06-04-2013 46 4   Download

Thec-jun belongs to the family of proto-oncogenes and encodes for the protein Jun, a component of transcription factorAP-1 involved in regulation of the expression of genes indispensable for cell proliferation and differentiation. While the role ofc-junin the regulation of such genes has been well examined, the regulation ofc-junin proliferating cells is not fully understood.

11p awards 05-04-2013 31 3   Download

The structure of the tryptophan synthaseb2 subunit (Pfb2 ) from the hyperthermophile,Pyrococcus furiosus,was deter-mined by X-ray crystallographic analysis at 2.2 A˚ resolu-tion, and its stability was examined byDSC. This is the first report of the X-ray structure of the tryptophan synthaseb2 subunit alone, although the structure of the tryptophan synthase a2b2 complex fromSalmonella typhimuriumhas already been reported. The structure ofPfb2was essentially similartothatoftheb2 subunit (Stb2)inthea2b2complex from S. typhimurium....

12p dell39 03-04-2013 53 4   Download

In order to estimate the size of the cavity remaining around the heme of the 3A3–microperoxidase 8 (MP8) hemo-abzyme,the formation of 3A3–MP8–Fe(II)-nitrosoalkane complexes upon oxidation of N-monosubstituted hydroxyl-amines was examined. This constituted a new reaction for hemoabzymes and is the first example of fully characterized Fe(II)–metabolite complexes of antibody–porphyrin. Also, via a comparison of the reactions with N-substituted hyd-roxylamines of various size and hydrophobicity,antibody 3A3 was confirmed to bring about a partial steric hindrance on the distal face of MP8....

7p dell39 03-04-2013 35 5   Download

The cytochromebc1 complex of the yeastSaccharomyces cerevisiae is composed of 10 different subunits that are assembledas a symmetrical dimer in the innermitochondrial membrane. Three of the subunits contain redox centers and participate in catalysis, whereas little is known about the function of the seven supernumerary subunits.

10p dell39 03-04-2013 56 4   Download

We have examined the status of the cytochromebc1 complex in mitochon-drial membranes from yeast mutants in which genes for one or more of the cytochromebc1 complex subunits were deleted. When membranes from wild-type yeast were resolved by native gel electrophoresis and analyzed by immunodecoration, the cytochrome bc1 complex was detected as a mixed population of enzymes, consisting of cytochromebc1 dimers, and ternary complexes of cytochromebc1 dimers associated with one and two copies of the cytochrome c oxidase complex....

14p media19 04-03-2013 39 2   Download