FACS analysis
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Ebook "Immunocytochemical methods and protocols (Second edition)" are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization.
447p coduathanh1122 27-03-2024 3 1 Download
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Right ventricle (RV) dysfunction and hypotension can be induced by high levels of positive end-expiratory pressure (PEEP). We sought to determine in an animal model if a novel ultrasound analysis technique: speckle tracking echocardiography (STE), could determine deterioration in RV function induced by PEEP and to compare this to a conventional method of RV analysis: fractional area change (FAC).
9p viisaacnewton 26-04-2022 8 1 Download
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While we are taught to never judge a book by its cover, covers may actually be revealing. In the case of a cell, the surface proteins on its “cover” are unique to particular cell types: for example, CD3 for T cells and CD19 for B cells. With such markers in hand, populations of cells can be classified into the cell types they contain, in particular using fluorescence-activated cell sorting (FACS) analysis with a panel of antibodies.
3p vialfrednobel 29-01-2022 14 0 Download
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Transgenic zebrafish lines with the expression of a fluorescent reporter under the control of a celltype specific promoter, enable transcriptome analysis of FACS sorted cell populations. RNA quality and yield are key determinant factors for accurate expression profiling.
16p visilicon2711 20-08-2021 11 1 Download
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Vitamin C (VitC) is a potent antioxidant and contributes as an apoptosis inhibitor by preventing death receptortriggered caspase 8 activity. Fas ligand (FasL) induces the apoptotic cell death via activation of Fas signaling, which is dependent on the expression level of anti-apoptotic molecule c-FLIP (FADD-like IL-1beta-converting enzymeinhibitory proteins). The present study addressed the effects of VitC on survival of dendritic cells (DCs), a regulator of innate and adaptive immunity.
7p nguyenxuankha_bevandan 14-08-2020 17 3 Download
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Proanthocyanidin (PAC) is a promising compound that has displayed its potent antineoplastic properties with a specific intrinsic pathway. This precise us to explore the phyto-preventive effect of PAC against colon cancer (HT-29).
14p vijiraiya2711 27-05-2020 18 1 Download
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social media marketing is a recent addition to organizations’ integrated marketing communications plans. integrated marketing communications is a principle organizations follow to connect with their targeted markets. integrated marketing communications coordinates the elements of the promotional mix;advertising, personal selling, public relations, publicity, direct marketing, and sales promotion. [1] in the traditional marketing communications model, the content, frequency, timing, and medium of communications by the organization is in collaboration with an external agent, i.e.
20p thamgiacongdong 02-05-2013 49 8 Download
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The innate antiviral response requires the induction of genes and proteins with activities that limit virus replication. Among these, the well-character-ized interferon b (IFNB) gene is regulated through the cooperation of AP-1, NF-jB and interferon regulatory factor 3 (IRF-3) transcription fac-tors. Using a constitutively active form of IRF-3, IRF-3 5D, we showed previously that IRF-3 also regulates an IFN-independent antiviral response through the direct induction of IFN-stimulated genes.
12p fptmusic 11-04-2013 28 1 Download
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Dendritic cell-specific intracellular adhesion molecule-3-grabbing noninte-grin (DC-SIGN) is a member of the C-type lectin family selectively expressed on immune-related cells. In the present study, we performed a systematic interaction analysis of DC-SIGN and its related receptors, DC-SIGN-related protein (DC-SIGNR) and liver and lymph node sinusoi-dal endothelial cell C-type lectin (LSECtin) using frontal affinity chroma-tography (FAC).
17p viettel02 19-02-2013 40 2 Download
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Phage display of antibody libraries is a powerful tool for antibody discov-ery and evolution. Recombinant antibodies have been displayed on phage particles as scFvs or Fabs, and more recently as bivalent F(ab¢)2.We recently developed a technology (E-clonal) for screening of combinatorial IgG libraries using bacterial periplasmic display and selection by fluores-cence-activated cell sorting (FACS) [Mazor Yet al.
13p mobifone23 21-01-2013 46 3 Download
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The results above have several important implications for the international finance and diversification literature. First, while our analysis of interna- tional stock return comovements reveals significant weaknesses of theHeston– Rouwenhorst model, when viewed as a factor model, we also show that the Heston–Rouwenhorst empirical results regarding the primacy of country fac- tors stand the test of time.
38p bocapchetnguoi 05-12-2012 44 3 Download
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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: SuperSAGE analysis of the Nicotiana attenuata transcriptome after fatty acid-amino acid elicitation (FAC): identification of early mediators of insect responses
16p panasonic06 25-12-2011 43 3 Download