Flow sorting

Ebook "T-cell differentiation: Methods and protocols" provides protocols to successfully apply cutting-edge technologies to characterize the biology of T cells at an unprecedented level of complexity. Chapters guide readers through flow cytometry and fluorescence-activated cell sorting, the behaviour of single T cells after adoptive cell transfer (ACT), single cell gene expression by multiplex PCR, lentiviral transduction approaches, protocols to derive large numbers of early-differentiated memory T cells by using dedicated cytokines cocktails,...

272p lucchinguyen 28-12-2023 6 2   Download

Ebook "Synthetic metabolic pathways: Methods and protocols" outlines key steps associated with the design, building, and testing of synthetic metabolic pathways for optimal cell factory performance and robustness, and illustrates how data-driven learning from these steps can be used for rational cost-effective engineering of cell factories with improved performance. Chapters are divided into four sections focusing on the four steps of the iterative design-build-test-learn cycle related to modern cell factory engineering.

352p lucchinguyen 28-12-2023 7 2   Download

Ebook "Algorithms in a Nutshell" includes contents: Chapter I Algorithms matter, Chapter II The mathematics of algorithms, Chapter III Patterns and Domains, Chapter VI Sorting algorithms, Chapter V Searching, Chapter VI Graph algorithms, Chapter VII Path finding in AI, Chapter VIII Network flow algorithms, Chapter IX Computational geometry, Chapter X When all else fails, Chapter XI Epilogue, Chapter XII Benchmarking.

344p haojiubujain08 01-11-2023 3 2   Download

Ebook Algorithms in C: Part 2 includes contents: Chapter 24: elementary geometric methods, chapter 25: finding the convex hull, chapter 26: range searching, chapter 27: geometric intersection, chapter 28: closest-point problems, chapter 29: elementary graph algorithms, chapter 30: connectivity, chapter 31: weighted graphs, chapter 32: directed graphs, chapter 33: network flow, chapter 34: matching, chapter 35: random numbers, chapter 36: arithmetic, chapter 37: gaussian elimination, chapter 38: curve fitting, chapter 39: integration, chapter 40: parallel algorithms chapter 41: fast fourier ...

313p haojiubujain08 01-11-2023 3 1   Download

Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes.

7p viaristotle 29-01-2022 10 0   Download

Single-cell RNA sequencing has emerged as a powerful tool for characterizing cells, but not all phenotypes of interest can be observed through changes in gene expression. Linking sequencing with optical analysis has provided insight into the molecular basis of cellular function, but current approaches have limited throughput. Here, we present a high-throughput platform for linked optical and gene expression profiling of single cells.

11p viarchimedes 26-01-2022 9 0   Download

Haynaldia villosa (H. villosa) has been recognized as a species potentially useful for wheat improvement. The availability of its genomic sequences will boost its research and application. In this work, the short arm of H. villosa chromosome 4V (4VS) was sorted by flow cytometry and sequenced using Illumina platform.

11p vilarryellison 29-10-2021 9 0   Download

Bacterial filamentation occurs when rod-shaped bacteria grow without dividing. To identify genetically encoded inhibitors of division that promote filamentation, we used cell sorting flow cytometry to enrich filamentous clones from an inducible expression library, and then identified the cloned DNA with high-throughput DNA sequencing. We applied the method to an expression library made from fragmented genomic DNA of uropathogenic E. coli UTI89, which undergoes extensive reversible filamentation in urinary tract infections and might encode additional regulators of division.

16p vitzuyu2711 29-09-2021 13 1   Download

Branching events in phylogenetic trees reflect bifurcating and/or multifurcating speciation and splitting events. In the presence of gene flow, a phylogeny cannot be described by a tree but is instead a directed acyclic graph known as a phylogenetic network.

12p vioklahoma2711 19-11-2020 7 0   Download

The objective of this study was to investigate whether or not AaGroEL communicates with T cells directly. To do this, sorted cells from peripheral blood mononuclear cells were stimulated with AaGroEL for 48 h. Flow cytometry was used to measure soluble and intracellular cytokine expression in the cell cultures and detect TLR2 expression on the surface of T cells.

8p nutifooddau 27-01-2019 22 2   Download

The aims of this study is to isolate potential candidate breast cancer stem cell from the MCF-7 cell line to find out whether it is possible to isolate cancer stem cells using two cell surface markers: CD44 and CD24. Stem-like properties were studied in vivo by observing the tumorigenicity of different cell populations in immunosuppressed mouse models. MCF-7 cells were subculture for several passages and were sorted out into 3 different cell populations by 2 specific antibodies which bind to CD44 and CD24, using flow cytometry.

7p jangni 16-04-2018 46 1   Download

In the general framework of a constraint-based grammar formalism often some sort of feature logic serves as the constraint language to describe linguistic objects. We investigate the extension of basic feature logic with subsumption (or matching) constraints, based on a weak notion of subsumption. This mechanism of oneway information flow is generally deemed to be necessary to give linguistically satisfactory descriptions of coordination phenomena in such formalisms.

8p bunmoc_1 20-04-2013 40 1   Download

Shimon Even’s Graph Algorithms, published in 1979, was a seminal introductory book on algorithms read by everyone engaged in the field. This thoroughly revised second edition,with a foreword byRichard M.Karp and notes byAndrewV.Goldberg, continues the exceptional presentation from the first edition and explains algorithms in formal but simple language with a direct and intuitive presentation. The material covered by the book begins with basic material, including graphs and shortest paths, trees, depth-first search, and breadth-first search.

204p hotmoingay3 09-01-2013 88 11   Download

In earlier book chapters, it was noted that the accounting profession uses an “all inclusive” approach to measuring income. Virtually all transactions, other than shareholder related transactions like issuing stock and paying dividends, are eventually channeled through the income statement. However, there are certain situations where the accounting rules have evolved in sophistication to provide special disclosures.

63p tuanloc_do 04-12-2012 51 3   Download

Sắp xếp và phân loại file trong Lion Finder Giống như những sản phẩm tiền nhiệm, Finder của Lion cung cấp cho bạn bốn cách để quan sát file: icon view, list view, column view và Cover Flow view. Mặc dù vậy nó cũng có một số cách sắp xếp mới các file bên trong khung nhìn này: Arrange By, Sort By và Clean Up By. Ba công cụ phân loại có ba chức năng khác nhau: Arrange By cho phép bạn tổ chức các file theo nhóm. Tùy chọn Sort By cho phép bạn thực hiện nhiều hơn những...

10p tieuyeuquai456 08-10-2012 81 3   Download

Some flow cytometers have the ability to physically separate different sub-populations of interest (cell sorting) depending on their cytometric characteristics (stream-in-air), thus permitting the recovery and purification of cell subsets from a mixed population for further applications (Bergquist et al., 2009; Davey, 2010).

374p yeutinh98 22-09-2012 81 12   Download

Contents Techniques r Introduction to Algorithms r Data Structures and Sorting r Breaking Problems Down r Graph Algorithms r Combinatorial Search and Heuristic Methods r Intractable Problems and Approximations r How to Design Algorithms Resources r A Catalog of Algorithmic Problems r Algorithmic Resources References Index About this document

0p khangoc2392 12-08-2012 46 2   Download

Quản trị mạng – Giống như những sản phẩm tiền nhiệm, Finder của Lion cung cấp cho bạn bốn cách để quan sát file: icon view, list view, column view và Cover Flow view. Mặc dù vậy nó cũng có một số cách sắp xếp mới các file bên trong khung nhìn này: Arrange By, Sort By và Clean Up By.

7p huongdanhoctot_6 27-10-2011 68 6   Download